LOCUS       sequence-448401-        8178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a form of full length SRGAP2 with one point mutation 
            (W765A) in the SH3 domain abrogating its ability to bind to 
            Gephyrin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, 
            Polleux F, Charrier C
  TITLE     SRGAP2 and Its Human-Specific Paralog Co-Regulate the Development of
            Excitatory and Inhibitory Synapses.
  JOURNAL   Neuron. 2016 Jul 20;91(2):356-69. doi: 10.1016/j.neuron.2016.06.013.
            Epub 2016 Jun 30.
  PUBMED    27373832
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.06.013"; journalName: "Neuron"; date: 
            "2016-07-20- 20"; volume: "91"; issue: "2"; pages: "356-69"
COMMENT     Sequence Label: pCAG_SRGAP2A_SH3(W765A)_HA
FEATURES             Location/Qualifiers
     source          1..8178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(413..493)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(3783..3802)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          3810..4826
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(3864..3886)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(4827..5103)
                     /label=chicken beta-actin promoter
     enhancer        5105..5484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5515..5619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5620..5688,5689..6480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5838..5857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6651..7239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7140..7159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7393..7410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7478..7612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7502..7521)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7556..7575
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7618..7813)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7632..7767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7872..7893
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7908..7913,7914..7931,7932..7938)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7946..7962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7951..7973
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7970..7986
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7970..7986
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctcagtggta tttgtgagcc agggcattgg ccacaccagc
      361 caccaccttc tgataggcag cctgcacctg aggagtgaat tgcggccgct taagcgtaat
      421 ctggaacatc gtatgggtaa gcgtaatctg gaacatcgta tgggtaagcg taatctggaa
      481 catcgtatgg gtatccccgg gcccgcggta ccgtcgatcg agcgacagta caagacttgt
      541 cagtagactg tggggaagtt gatgagttga caccagggct agtggcattt gttttgggga
      601 agacagtggg cttgggccgt gtggctggtg gtttgaccgg ggcagccatc ttgacagact
      661 tgacaggccg gaaggcgcag gcgatgtccc cagcagtact ggcgctgcgc tggaaggcgg
      721 gctcggggtc cttgaggagc tgggtgtgca gagggctgga gggctctgac gtgggggcca
      781 ccactgggga ggttttgagg ggctccaagg tgtccagaac cacgtcaggg gtgtgtttga
      841 cactgctctg ccgttctagt tcccgtagct cattcagggc cgagttcatt gttgcctcaa
      901 tatcctgagc aatgacctca gggtccatgg gccgatggtt attgaagctt tttgaccttc
      961 ccgctgtggc agtcttccgg atctgtggac tatccagccg attcttcagg gatgagtggc
     1021 ggctgatgga gttgaggctc ccgtgcccac tgatggaaca cttatctggc ccttctttgg
     1081 ggaggctctg gctcatgatg ggctgggagg atgggcggca gctagccccc acccctgggg
     1141 aggaggagtc agtcagggaa ctgctcagcc catggctgtc actccgaaaa gttttccgga
     1201 tgctcccaga ttctggacgc ttcctttgct tgttgatgtt tgcaagataa atatcggcta
     1261 catgaccccc actgggacag ctggcccccg ctctggctgt caccttttct tcaggtggct
     1321 cagaaatgac ctcaatctca gacttggggc tggacctctc cacgacaccg tcctcggtgt
     1381 cttggaccac gatgtactga tgggggatga gtccgtcgat gccattgtgc cggccttccc
     1441 acgcgtcgtc ggaagcccgc tggtaaagca gcagggatgc tcccttctta aaggacagct
     1501 ctcgggctgt ccggcccacg tagtcaaact tggcaatggc ctcgatgggc tcacattcgt
     1561 catcgctcgt gtggtgctct gcggtcacat cctgggttga gtcttcaacc gaggtagtct
     1621 ctccatgagg gctatcacag taatcctcca tgcttcctcc tctgctgtag acagggccct
     1681 ccagctccct ggggcttggg aagatgttct catgctggat gatgatggtt ttgatcagct
     1741 cattcacgtg ggcttggcag gacacctggt cgtggccctc tggcactgac attagcgagg
     1801 gcccgaagca gatggcgagg ttgtaggggt ccatcatgtt ctcttcactg aactgtgata
     1861 aatgattgag gaaggcaaag aggtatctca tgataatcag agtggttttg ggcaggacta
     1921 ggaggacttt ccggatgtgc agagctctct cctgcaggtt gtccattgtg acgcaggcca
     1981 tcaggtcatg aaagatgtcc ttggggaaga gagggtgttc cagcccccgg aagtaaagct
     2041 tcaggacacc agctatggaa tccatgtcat ggtcgttctg gtccccagcc agggggtcct
     2101 ctcctctctc aaaggcattt ttgatgtcat tcacttccac ctgggatcct gacacccgga
     2161 aaattccttc atgctgtagt ccgtgtctgc tgataaaccg gatacagctt tccaccacca
     2221 gaggaattgc ctggctggag tcctgtttcc tcacagttga gctgcgcctg gctagactgc
     2281 aatctgtccg ctgactttct cccagggttt tctgcagaag gtcatgcttg gcttgtaact
     2341 tggtgatgag gttcctgccc tccaggtact ctttcatttt tgtgaaataa aactgctctg
     2401 tctcttgctg gttggctctc ctcttagcaa tgctgggctt gctcatgaag gtttcagaga
     2461 ccgtggactt gacggactcc atggagttgc tgtactggaa gcagtcagac acatcaaagt
     2521 cctcgacagt cacaatgtcc tggatggttt gcagggtggc ctccattgtc ttctttacct
     2581 cttcgttttc aatctttaga gtggataagc gagactgcag ttgttggcat ctctgtacca
     2641 gctcactctg gacaggctgc tgggcacaga gctgggaagc catatccccc atgtggggct
     2701 gaaactcaaa cttcataggg gggcagaaga cgttgttgta catctccatg aggcgctgct
     2761 tgtcactggt ggcatccagg ttttctactg cattctcgat ggcatccaga ccctcatgct
     2821 tcgactgttc caggtttaac tcagcagaga ggaaggtgcg tagagcccgg ttcagacttg
     2881 catggtagcc taagtcacaa cactgatcaa taaggtcaga taggtcatgg atgtagtact
     2941 tgaagacaga tgcattggtt gcctccaaag ccagcaagta ctcattccgg gctttgatgg
     3001 ccttcagctt attctccgtg tacttggctt gacgcttctc cttcatcttc tcaatcttct
     3061 tcactgagct cctccggaca tgtttctcct caatgcgaac gttggccgtg gagtcagggg
     3121 agcgtggggt ctgccggtcc tcctgcttta ccgatttacc aatttgcttc tcctcctgct
     3181 tctccgcctc ctttagtttg ctctgagcac tgatgctgtc ggcattgtac atgtgatatg
     3241 tcttcatcac cgagtagagc tcgttcagga ccttcatcaa atcatcttgg agctgctggc
     3301 cgacttcttt actcttttta aagagtcttc ctgagtcctc gctgacttgt acaaatcgag
     3361 gaatgatatt attcaggtag atgtcactca gggtggtatg gtccctgctt tcccgcttca
     3421 cctggtttaa gaggagattc cagcagttga ctggagagag aacattctga tccttcttga
     3481 attgctggtc cttggtgctg cgtgtcttgg ccaggaagcg ttctgccagc ttctccaggt
     3541 tgcgggagta gtccatctca atctctgcct tctttcggaa gaagtcctgg aggtcctgca
     3601 acagttgcac ccgaagctca cactgctggt ccaggcattt catctgctct gtgagctgag
     3661 cacggatctc tttgacctga gtatcgtact ctgctatgat ctccttatcc tttttgaatt
     3721 tggctggaga cgtcatgaat tagcttgagc tcgagcaatt ctttgccaaa atgatgagac
     3781 agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg
     3841 ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt
     3901 cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga
     3961 cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac
     4021 cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca
     4081 cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg
     4141 ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg cccctccccc
     4201 gagccctccc cggcccgagg cggccccgcc ccgcccggca cccccacctg ccgccacccc
     4261 ccgcccggca cggcgagccc cgcgccacgc cccgtacgga gccccgcacc cgaagccggg
     4321 ccgtgctcag caactcgggg aggggggtgc agggggggtt gcagcccgac cgacgcgccc
     4381 acaccccctg ctcacccccc cacgcacaca ccccgcacgc agcctttgtt cccctcgcag
     4441 ccccccccgc accgcggggc accgcccccg gccgcgctcc cctcgcgcac actgcggagc
     4501 gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc cgggcagcgc ggagccgcac
     4561 gcggcgctcc ccacgcacac acacacgcac gcaccccccg agccgctccc cccgcacaaa
     4621 gggccctccc ggagcccctc aaggctttca cgcagccaca gaaaagaaac aagccgtcat
     4681 taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct cacctgtggg
     4741 agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcggcgga gcggggcacg
     4801 gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc
     4861 cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg attggctgcc
     4921 gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg
     4981 cgcccccccc cccccccgcc cccatcgctg cacaaaataa ttaaaaaata aataaataca
     5041 aaattggggg tggggagggg ggggagatgg ggagagtgaa gcagaacgtg gggctcacct
     5101 cgaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     5161 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     5221 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     5281 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     5341 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     5401 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     5461 aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga
     5521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7321 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7381 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7441 cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga taagatacat
     7501 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7561 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttagcttttt
     7621 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     7681 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     7741 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     7801 ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7861 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7921 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7981 tatgaccatg attacgccaa gctggtcgac ggtgcaccgt cgaccagctg gtcgacggtg
     8041 caggtcgagg gatctccata agagaagagg gacagctatg actgggagta gtcaggagag
     8101 gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa
     8161 taacacaaaa caaaatat
//
