LOCUS       sequence-44841-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN40-R4R3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(92..98,99..116,117..122))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(147..270)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(278..295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(278..294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(287..309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
       61 aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta
      121 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
      181 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      241 tttctcgttc aactttatta tacatagttg ataattcact ggccgtcgtt ttacaacgtc
      301 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      361 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      421 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      481 accgcatatg ccgggcccca ttttatatct gagtagtatc ctttgcttta aatgtccata
      541 aaactaattt tataatcaat aaaacaacgt ttgtaaatca actgagttta caagtagaga
      601 cattgaggga tactttcact atgctaaagt gataatcgac caaataataa cttcactttg
      661 gtatttattc ctgtcttata aatgttatgt atgaattaaa ttcatatgca tatggctcac
      721 tctgacaatt tttaataatc ttccagatca atattgacta ccgatgcggg tggtcttttg
      781 ctttgaattc tgctgaactt tacaccccga acagcaatgt gtgcttcagc ctaaaaaaaa
      841 gtaagtgtgt taatcagtgc ccccgattct tcat
//
