LOCUS       sequence-44843-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN40-R4R3
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     650..672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(710..728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
       61 gacgagttct tctgagctag ccggccatac aagtaatccg gatgatcgac gccaacgtcg
      121 ttgaattttc aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct
      181 ctttgtgttt tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatttttt
      241 tttctctttc cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt
      301 ttatttatct tctcgctttc atttcattag gacgtggggg gaattggtgg aagggggaaa
      361 cacacaaaag gatgatggaa atgaaataag gacacacaat atgcaacaac attcaattca
      421 gaaatatgga ggaaggttta aaagaaaaca taaaaatata tagaggagga aggaaaacta
      481 gtccaagatc tcagtggtcc taggtgagct cccatatggt gcactctcag tgcaatctgc
      541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
      721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      781 tttcggggaa atgtgcgcgg aacccctatt tg
//
