LOCUS       sequence-448432-       12408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CIRTS RNA targeting system for targeted knockdown of m6A-containing 
            transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is 
            expressed under human Syn1 and U6 promoter, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Madugalle SU, Liau WS, Zhao Q, Li X, Gong H, Marshall PR, 
            Periyakaruppiah A, Zajaczkowski EL, Leighton LJ, Ren H, Musgrove 
            MRB, Davies JWA, Kim G, Rauch S, He C, Dickinson BC, Fulopova B, 
            Fletcher LN, Williams SR, Spitale RC, Bredy TW
  TITLE     Synapse-Enriched m(6)A-Modified Malat1 Interacts with the Novel 
            m(6)A Reader, DPYSL2, and Is Required for Fear-Extinction Memory.
  JOURNAL   J Neurosci. 2023 Oct 25;43(43):7084-7100. doi: 
            10.1523/JNEUROSCI.0943-23.2023. Epub 2023 Sep 5.
  PUBMED    37669863
REFERENCE   2  (bases 1 to 12408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0943-23.2023"; journalName: "J Neurosci"; date: 
            "2023-10-25- 25"; volume: "43"; issue: "43"; pages: "7084-7100"
COMMENT     Sequence Label: CIRTS-YTHDF2-control
FEATURES             Location/Qualifiers
     source          1..12408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3726..3735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3777..3798)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4038..4057)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4385..4406
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4458..4511
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5028..5054
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5688..5711
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6745..6993
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6745..6765
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6916..6935
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    7100..7688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7153..7173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7571..7582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7691..7707)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7692..7708)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8213..8393
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8419..8436)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8425..8649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8695..9123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8782..8801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8992..9013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9132..9152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9137..9466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9317..9452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9379..9398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9514..9561
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9580..9954
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10084..10205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10121..10140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10175..10194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10254..10270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10254..10270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10267..10289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10278..10294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10302..10308,10309..10326,10327..10332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10347..10368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10485..10502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10656..11244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10736..10755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11415..12206,12207..12275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12038..12057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12276..12380)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggttttgg ctacgtccag agcagaggaa tgagggcatg
     2641 tagactaaat atgttcgtgt ggaagaggct gaatacacat cagagttagt gctgcaggaa
     2701 atgcttctgc attgcatacc cagagtttcc ttgctcatct gagagcatgt gttttttcca
     2761 gatgtgtgta cttgtgtgag attctctggg tgtgtgtcaa tgtgttgcct gaacgtgcat
     2821 tgctcaatat gctcatgtgt gttaccctgg gcttgtacat ctacatatat acctggatgc
     2881 ccgtgtgttc tgtgatgtac atataccctg tgtcattcct tgtttttcta tttgtgttat
     2941 tccatgtgtt ccttcaggct ctcactaccc aagtgtccac ctccgcctgt ctggtgatgt
     3001 ttacgctacc ccgtgctctt ttctttgcct gacagtgttg tcgtggaaga catctcgcca
     3061 ggaacactgc agtaaggaga atttctagtt ttatgttccc ctccgagtat gcttctatcc
     3121 cgaccctcaa ccccaaaatg ccttcagagg tgaaaatcaa cactggaaac acaagtatct
     3181 gggaagggta acaatgcaag ttagcctgag gatttaggag gaggctgaaa aacagagtag
     3241 gagccttact acgggtccag accctacgga caagaacccc cactcccact ccccaaattg
     3301 cgcattccct cccccatcag agggggaggg gaagaggatg cagcgcggcg cggcgcgtgc
     3361 gcactgtcgg atttagtacc gcggacagag ccttcgcccc cgctgccggc gcgcgccacc
     3421 acctccccag caccaaaggc gggctgacgt cactctccag ccctccccaa actcccctac
     3481 ctcaccgcct tggtcgcgtc cgtgcagcgg tgagtccagt cgggccgcac cacaagaggt
     3541 gcaagatagg ggggtgcagg cgcgaccata cgctctgcgg cggcagagcc tcagcgctgc
     3601 ctcagtctgc agcgggcagc agaggagtcg cgtcgtgcca gagagcgccg ccgtgctcct
     3661 gagccccttg cgctccgccc ccgcggccca ccgacccact gccccttgga tccccgggta
     3721 ccggtgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3781 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3841 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3901 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4141 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4201 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4321 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4441 tgtacaaggg ctccggcgag ggcaggggaa gtctactaac atgcggggac gtggaggaaa
     4501 atcccggccc aaccggtatg ggaattatca acacccttca aaaatactac tgtcgcgtcc
     4561 gtgggggtcg ttgcgcagtc ctgtcgtgcc tgcctaaaga ggagcagatc ggtaaatgct
     4621 caacccgtgg acgtaagtgc tgtcgtcgca agaaaggcgg ttctggaggc tcagggggga
     4681 gcggagggtc cggtggatca ggaggctcca tggcagttcc cgagacccgc cctaaccaca
     4741 ctatttatat caacaacctc aatagcaaga tcaagaagga tgagctaaaa aagtccctgt
     4801 acgccatctt ctcccagttt ggccagatcc tggatatcct ggtaccgcgg cagcgtaccc
     4861 cgaggggcca ggcctttgtc atcttcaagg aggtcagcag cgccaccaac gccctgcgct
     4921 ccatgcaggg tttccctttc tatgacaaac ctatgcgtat ccagtatgcc aagaccgaca
     4981 aacgtatccc ggccaagatg aaaggcacct tcgtgggatc ccttcaactg cctccacttg
     5041 aaagactgac actgggcggt agtggaggaa gcggggggag cggagggagt ggtggatccg
     5101 gcggctccat gtcggccagc agcctcttgg agcagagacc aaaaggtcaa ggaaacaaag
     5161 tacaaaatgg atctgtacat caaaaggatg gattaaacga tgatgatttt gaaccttact
     5221 tgagtccaca ggcaaggccc aataatgcat atactgccat gtcagattcc tacttaccca
     5281 gttactacag tccctccatt ggcttctcct attctttggg tgaagctgct tggtctacgg
     5341 ggggtgacac agccatgccc tacttaactt cttatggaca gctgagcaac ggagagcccc
     5401 acttcctacc agatgcaatg tttgggcaac caggagccct aggtagcact ccatttcttg
     5461 gtcagcatgg ttttaatttc tttcccagtg ggattgactt ctcagcatgg ggaaataaca
     5521 gttctcaggg acagtctact cagagctctg gatatagtag caattatgct tatgcaccta
     5581 gctccttagg tggagccatg attgatggac agtcagcttt tgccaatgag accctcaata
     5641 aggctcctgg catgaatact atagaccaag ggatggcagc aggatccgat tacaaggacg
     5701 acgatgacaa gtaggctagc gggagcctct gccctcctcc tgcagcccgg atggctctcc
     5761 tctgtttttg gtttgcttcc ttttgtttgc ccttcttttg ggtgaccggg gtggccacca
     5821 ttcctgtccc tgcccagtgg ggaggggagc aaggccttct gccaagagga ggagcatgtc
     5881 ccctgccact gttgcatgca ccccggccct gtgactctgt gtgcaagccc agcagcctgt
     5941 ggggcagggt gccaagaggg gacgggacgg acgttccagg acagagggag tcgaggatgt
     6001 gatccaggac gggtgggggg tggaagggtg ggaaagggca ggaaggcctt ggaagctaac
     6061 atgctttcac tactggaggg ggacggatag agcggatggc actcgggtct cactgacgct
     6121 gttctgaaac catctggctg gctttctgag gtcaggctgg gtagggggct gtcatcggcc
     6181 atactgctga tcacacttgc cctcatccgt ccagcgatct cacgctgttc tgtaaatacc
     6241 tggtgctaac atcccatgcc gctcccccat ggcaccccca gcctcctgcc cagttcaggc
     6301 tggtccagga atggatgtgg gggatggtcg ctgtgtaacg tgctggtgat ctttttcccc
     6361 tacccctttt atgaccctta aaactttgat tttgtcctaa acatgccggg ccaactaaag
     6421 ggtggggaga ggagagacga gccccaccat gctcacaaga accaacctgc aataaacact
     6481 tctgtgggcc gccgcgcaga gcacgcagcg ggcacctcct gcccctccat ggcgacttgg
     6541 catcgctttc tgcccgcctc tgccctcgcc ctgcctgccc caccccccag cggagagcat
     6601 gatccgcgcc cttgcttctg actctagcct ctgggacaca tcagtcaacg tgggcagttc
     6661 agtctgggtt cgttcctttc tctgttctca tctggccccc cagggcgatc ttgttgcttt
     6721 ttgctgggac ctgcccagtc tagagagggc ctatttccca tgattccttc atatttgcat
     6781 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
     6841 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     6901 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     6961 gctttatata tcttgtggaa aggacgaaac accggccaga tctgagcctg ggagctctct
     7021 ggccttattg atacatcatc tctgtattag gctcccaact tttttgagac gtctagaatt
     7081 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7141 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7201 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7261 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7321 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7381 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7441 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     7501 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7561 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7621 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7681 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     7741 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     7801 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     7861 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7921 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7981 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     8041 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     8101 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     8161 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     8221 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8281 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8341 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     8401 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     8461 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     8521 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     8581 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     8641 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     8701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     9001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9121 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     9421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     9481 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     9541 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     9601 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     9661 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     9721 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     9781 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     9841 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     9901 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     9961 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    10021 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    10081 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10201 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    10261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    10321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    10381 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    10441 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    10501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    11041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    11101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    11221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    11281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    11341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11701 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12001 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    12241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    12301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
