LOCUS       sequence-448438-       11197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minigene encoding the ABCA4 c.5461-10T mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Tabaglio T, Agarwal T, Cher WY, Ow JR, Chew AK, Sun PYQ, Reddy 
            Gurrampati RS, Lu H, Naidu P, Ng HK, Le Guezennec X, Ng SY, 
            Lakshmanan M, Guccione E, Wee KB
  TITLE     Unveiling sequence-agnostic mixed-chemical modification patterns for
            splice-switching oligonucleotides using the NATURA platform.
  JOURNAL   Mol Ther Nucleic Acids. 2025 Jan 7;36(1):102422. doi: 
            10.1016/j.omtn.2024.102422. eCollection 2025 Mar 11.
  PUBMED    39926316
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2025-01-7"; volume: "36(1):102422. doi"; 
            pages: " 10.1016/j.omtn.2024.102422. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pRP.ABCA4 c.5461-10T
FEATURES             Location/Qualifiers
     source          1..11197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        25..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..684
                     /label=chicken beta-actin promoter
     intron          685..1701
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1625..1647
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1709..1728
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1755..1779
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8901..8925)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8954..9075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8965..8984)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9019..9038
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(9191..9208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9362..9950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9442..9461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10121..10912,10913..10981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10744..10763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10982..11086)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11154..11172
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caactttgta tagaaaagtt gctcgacatt gattattgac tagttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      421 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      481 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggggc gcgccggggg
      541 gggggggggg ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
      601 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
      661 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
      721 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
      781 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
      841 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
      901 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
      961 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     1021 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     1081 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     1141 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     1201 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     1261 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     1321 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     1381 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     1441 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     1501 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     1561 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     1621 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     1681 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     1741 tttggcaaag aattgcaagt ttgtacaaaa aagcaggctg ccaccatgaa ttattccgtg
     1801 agtgctgggc tggtggtggg catcttcatc gggtttcaga agaaagccta cacttctcca
     1861 gaaaaccttc ctgcccttgt ggcactgctc ctgctgtatg ggtaagccgt ttgggccatt
     1921 agctaatgcc tctgaagaga agcctggtgg tgggggtggg ggatcatctc ctgacagaaa
     1981 acctgggctg tcctgtggtg gtagcaccca caagtttagc ttccggcccc aggtagggtc
     2041 tgaagctgat aaccagggat ctgtctggct tctgattctg actccactga cagaggtatc
     2101 tctgaggcct ggtcctgtca gtgacaatga gagaagtccc acatgatctg aatctcctac
     2161 tcaaactgag gccttgacca aagcctgggg gcagccattc cccaacccct cacccagctc
     2221 tgactctcac tcatctgtgg ccaatctgtc cacctcagtg tccccatgtg aactggccaa
     2281 gagttaccgc ccacagtaga agactccggc caaaaagctc ctcctgagtc agggacagag
     2341 gatgacacag gggttacatc agcagagtta cagggcccag catgcaactt tctttcccac
     2401 gtgtgtaaat ttgaatgagt aattcatcca tctcggcctc agtttcctca tctgtaaaag
     2461 aaaatagtga tcctggtcct tcctctgtgg gccagtagag ccttgccaaa gcattgttct
     2521 ccacatcttt ctcttggaaa tagagaattt gggaaccaac ctgactataa gctgtgaaga
     2581 tgagctcact gggctcatct gagatgacct cagctgggct ttgctgaccc aggctagagt
     2641 gggaggtgtt gcaggctgga gaaccctcct atgaattgta cagggctttg tagtttacag
     2701 agtatataca cagctagcag cccatttgct cctcacaaaa ccccatgaag tggtcaaggc
     2761 aggcatcatt atctccattt aaagttgagg cacagagacc aacaaatgga gtatctctct
     2821 ggtcccctgg gactctggcc agttcacaca catcacctca ggtgtaaggg gagtgcatta
     2881 tatccagacg tattgtaggt ggaatggaat gtggaactcc atcactctga gttgtctcat
     2941 ttcacacaga tgggcggtca ttcccatgat gtacccagca tccttcctgt ttgatgtccc
     3001 cagcacagcc tatgtggctt tatcttgtgc taatctgttc atcggcatca acagcagtgc
     3061 tattaccttc atcttggaat tatttgagaa taaccgggtg agcataactt tcttggcttt
     3121 tttgtttgat tagtaggata gtagagtatg tgttggtcga gcagagccag gggcaagcat
     3181 cgtacatgta gcagctgtat gcggatgagt gccactttct tcctccctac ccccgaccct
     3241 gcctcctttc cttccttcct tcctcccatc cttccttcct ctttccttct tctcctccct
     3301 cctccctcct tcccccgtcc ctccttcctt cctttttcat tgcttccttc cttccttcgt
     3361 ccctccttcc cttcctcttt ccttctgccc tctctccctt tttcctttca tcctccctcc
     3421 atccctccct ccatccttcc ttctttcttc cttctttcct tcctataagc acctttttca
     3481 tttctgtgct ctgaatgaaa tggttttctg tgtttattct gcaagcaaaa cttgattctt
     3541 gcaataaact ttaagctttg cttactcttt cagaaaggtt ttctcaggga ctttgggtgt
     3601 tgggttttac acacacacac atcaatacat ttgggtaatt tcaaaatcta aaaggaacaa
     3661 aaaggcatac aatgaaaaaa tctccttcct acccctgttt cccactcatg cagttctctt
     3721 ctccagaggc aaactcttac ttgagtttcc tgtgtgctct ggagacacat cagcagatcc
     3781 ctatacggtc tttctcccgc tttcttatgg aaattgtaac actctgacat atactattcc
     3841 ttgggcaagt taatcttgat gaagagactg ggtgttctcc atgctgaatg cctcactttt
     3901 atgagctgcc aagcccagtt gtcccttcca cctgacctcc ccctgtccag agacagatgg
     3961 ccaaactgaa tcataaaaag agggggaaaa aaagaaggca gtcgctgcag ggctgtcttt
     4021 actccacact ccacactccc agtccccacc gctgtgtctg agtcctggct gtggctgtcc
     4081 ttggaacatt tgcctcacca cgtgcctgtg tccccaggcg cctcaacctt tcctctcctc
     4141 attagctctt cccagttcag agggtgggac cggccagcac atctgcactg ctgccctgcc
     4201 acacccacct ccacctgcct ctgggcccca ctggggaaca caggacaaat ctgtgcggag
     4261 gccccaccat gaaccgccca gacccgtgga cccctgagac tgactctttc cagatcttgt
     4321 tagggtttcg tggctgctag gcaagtaacg aagcctcatc tgtcccatga atgataagaa
     4381 attcagcatg tcagagtcag actctggaaa ggcgggggga taagaacaca gccccagcag
     4441 atggccagag cacccaggtg actgaaagtg ctgctttgca gagctgtgtt tgccacaggc
     4501 tcacagccca ctaagtctta agacagtttt ccttcagaat aattaaatag ccagcttaaa
     4561 gcaactcaga acattttccc ctctgaggct gcacccattt agccaacatt tgctaagcac
     4621 ccgccttcaa aaacctggta ttttcatgta aattatccga tacacagctg ctatggaaac
     4681 ccccagtatc ccacaggaag ctccccagct cccagcagct gccggcccgt gtgagatcag
     4741 gaggtcttta ccagctgaac accacgtgcc gggtgtgtgc tgatataaac aagcgtggcc
     4801 cactcgtcct gccctccaga ggctcccgtt ccagtcggaa aaggacctgc ccacgaagtt
     4861 tgcaacgata taagccacag tgtatgatcc tccataatac agcgtgtgac agagcagcag
     4921 aggagcgagg cagataacat gctgcaggcc agaggcagcg ggaagagcca ggctgcaggg
     4981 gctgggggag ccgtggtgga ggaagttcaa tttcagcctg tagatttcta ttagcccatt
     5041 taataaataa tgaagtgcct actctgagct aatcattgtg caggtattta ggaaggacaa
     5101 aaaaataatt aggactcagt gcccaccctc caggggccca ctgactagta gagaaagtag
     5161 gcagattttt aaaaaattaa tcatgggaat gtgataagtg ctgggagaga ggaatggata
     5221 ctttctcatg ggaatcttgg aaggcttgta agggaaggca ctctctgagc cagctgtcta
     5281 aagaagaaca ggaatcttta agaaagcaga agggaaaaga gcattctttc ctgcttggag
     5341 caataggtaa cagcctgcac atgcccaggc ctagaggcca aagagcacag tgattccaga
     5401 aagagtgggg agaaagggta ggcagggaag gatgaggtaa tgtgggcgca ggtgtggagg
     5461 ctggagaggg aggaggttgt gggactggga ggagccagat ggaatggaca gcagtggccc
     5521 agccaggagc tatgctggcc tcgtacgcct cgatgtccct tctattttct caggggaggc
     5581 tctgcccaac atgccaagtc cgaccacttg aaaacaagtc cctggcttaa cacagacccc
     5641 agagagagtc tccaaccctc ctctccctag acaatggtag ttgccctgtg aggggctgaa
     5701 aagcagagct ggagatggct cagggcctgg tgttaacaaa tgccttgagg gctcctgttg
     5761 tttcaaagtg agtctgcagg gagagctccc taagtggaca gcaggagggc tgcagcttct
     5821 ctgcacattc ctgctgtcac ccccagagtc acctagggga ggggtaagga cagtaatgca
     5881 ggttcctcac agttagcctc ggtgcccaca tggtactgag catagtaaat gtttagaaga
     5941 tgctgcctgg ctagacaaag gggaagctcc cgcccactag aaacttgcag ggagccccag
     6001 tccttgattg gtcatttaat tgattagctc cttggcctgg ccttgaggca ctgcttgtaa
     6061 gtacttcatg acctccattg caaacccatg atgctctgct ggacaaatcc ctccagtggc
     6121 cagtctggct gcaaggactc tctgtctgca ggccttgccc tgtgctgtcc tgtgagagca
     6181 tctgggcccc acctgctgaa gagagggggg gtggggtttg ccccgtttcc aacagtccta
     6241 cttccctgtt tcagacgctg ctcaggttca acgccgtgct gaggaagctg ctcattgtct
     6301 tcccccactt ctgcctgggc cggggcctca ttgaccttgc actgagccag gctgtgacag
     6361 atgtctatgc ccggtttggt gggtggtagc cgaggcccat ggagcatggg ccctgggtcc
     6421 aaagctggga gggttaccgg gggggctcct gcatcagact gtggcagggg ctggtgctag
     6481 gaggggacct tgttgggctg gaggtgtcct gccagctgga gaggattagg gtgcctctgt
     6541 ttccatggct ggggagccac aggagggatg gagggcagcc cttatgaggc gggtgtttgg
     6601 ctcttgctca gttcccacat aaggcctggt ctagtgggcc ctgtgctgtg gccaggtctg
     6661 tggggtgagc tggggcggct gaagtggact caattcctgt tgatgcccag gtgaggagca
     6721 ctctgcaaat ccgttccact gggacctgat tgggaagaac ctgtttgcca tggtggtgga
     6781 aggggtggtg tacttcctcc tgaccctgct ggtccagcgc cacttcttcc tctcccaatg
     6841 gtacgtccat gccacaccct gggccagtgg gcagctcagg gcatccagaa ctggacctta
     6901 tacccacatg gtcatttctt tcctcaggag ccccactcca caatgttttt tctacattct
     6961 caaagcctgg cttttctcca ataatacaag tagaggatcg ggttaaaata ggcacattca
     7021 aatatgtgaa gagcatccac tttaaaatat ttaaaatgca gtgctattaa tttcaattgc
     7081 tgatatttaa tccttctcat ttaattacca aatgtgtatt ttgattagat gatagtattg
     7141 caaataacaa tggttacagg gtatccaaag tactaggaaa tagactaatg tatttatgag
     7201 agaaaggaca cagcaggccc ctttgctaat tagagatttg ggagcatggg agtaatatgg
     7261 gagccatgtg gaggggtgcg ggcagtgatc acgacccccc actcctggag gaaggtgggt
     7321 agctgccaac cctgactttt gaccagggct tctcaaatgc caggttagct ggcaattgcc
     7381 attcttccgc aggctcttcc tgaagctggg tgggcccctg cctcactccc ctctgcaatc
     7441 cagtcctacc tttattgtcc tcacccaggg gcctgaattg ccaagcagca gcccttccta
     7501 gcaagctttc cccaatagtg ttttgtttct taacttttcc tcctctcagg ctgagtgtgg
     7561 tcacctgtaa atagattcca aggacttggt tttatgtttt gatccacagg gaattgattt
     7621 attggaaatg aatctgcctt tctactcaca ggactgtgag aggtgaatga gatcacaggt
     7681 gtcaacacac gcctgatgaa acaggataca caagcagttc tagttatggg agacagtgtc
     7741 aggaattgtt gtccttggca ccctcagccc ctgcagaccc tttctgcagc cttggccata
     7801 ccttttagag gcttttgtgt gggagagagc aggtcaggag gttgactacc caaattgact
     7861 cattagcttc aaactctgat gtcaacacat ttgaatgagt cctgcctgct ttagggccta
     7921 aagaggacca gagaagtaca ccatagtccc tggcttccag aaggtcaggg agggtttcaa
     7981 agaagaggct gtgtctttaa gaatggggaa gattccattt ggtggggcag gaggaggaga
     8041 acattgaggg actggaaaca catgcggagg ctgggagacg ggaatgacca ataggactgg
     8101 gaaccagggg gagatgccaa ttgctgacag aggagttagt gcaagaggta agtgagaagg
     8161 gtaggtgggg ctggattgca gggctgtaac tacagctgca gagggagggc ttcaacctac
     8221 agctgatggg gaacaacaga aggttttgag gcatgaggtg gcctgatgac aactctgttt
     8281 tggaaaggtg gagttggcag ggcagactgg aggaagtggg aggctcggag gttagtaact
     8341 accccttact gagtgcttgc tgtagaggaa gcattttagt cctgacggtg atcccaggcc
     8401 ctgagtcttt actctgtgcc aggcactgtg ctgagttcat cttcagcaca atcctatgag
     8461 acaggtattg ttaccctcct cctcatcaca tggttgaagt aggcaaggtt cagagaggtc
     8521 caatgcccaa gatcacacat gaggaggcca ggactggaac ccaaggctga ctctggacat
     8581 gagcacctga cctctctacc taatgcctaa tgcctctcct gctgggagcc ctttttagaa
     8641 tttaagtctt aaaggatgga agcccagaag gaagcagaag caaggaagtg gaagagaggt
     8701 cccatggaaa ggacagtgcc aaggacactg tacagccagc ccaatcctga ccccttttct
     8761 tcatctagga ttgccgagcc cactaaggag cccattgttg atgaagatga tgatgtggct
     8821 gaagaaagac aaagaattat tactggtgga aataaaactg acatcttaag gctacatgaa
     8881 ctaaccaagt agataagtga acccagcttt cttgtacaaa gtggtgatgg ccggccgctt
     8941 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     9001 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     9061 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     9121 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtagcgg ccgcggcgct
     9181 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9241 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9481 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc tcttcgggaa
     9541 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9781 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10021 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    10081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    10141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    10201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10261 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10381 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10441 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10501 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10561 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10621 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10681 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10741 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10801 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10861 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10921 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10981 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    11041 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    11101 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    11161 gtatcacgag gccctttcgt cggcgcgccg cggccgc
//
