LOCUS       sequence-448439-       14876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express OS9 gene in mesenchymal lineage of Zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:OS9_CE_pISceI
FEATURES             Location/Qualifiers
     source          1..14876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    6981..7115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7136..7270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(7572..8285,8286..8288,8289..8291))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7575..8285,8286..8288,8289..8291))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7617..7638)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7966..7985
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8225..8246
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9472..9488)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9472..9488)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(9543..9561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9571..9588)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9571..9587)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9580..9602)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9728..10183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9865..9886)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10077..10096
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10210..10314
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10315..10383,10384..11175)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10533..10552)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11346..11934
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11835..11854
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12088..12105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12222..12243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12258..12263,12264..12281,12282..12288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12296..12312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12301..12323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12320..12336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12320..12336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12355..12375
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12357..12375
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atattataca atcacaggca attaaaagat gtttaaagtt caatttagtt ttaattaata
       61 tgttatataa aactaatctg ttttaaatgt atatttatga ttttagcttt agctagcctc
      121 ttgaccagca gctgaagtgt ccggcaagta cctaaaaggg agaccacatg ggaaagctag
      181 gttgctcccg gaagaggtgt tagtaaacca gcaggtctac ctgtggtctg tgtgggtcct
      241 aatgccccag tataaaccag caggtcaacc tgtggtctgt gtgggtccta atgccccagt
      301 ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta taaaccagca
      361 ggtcaacctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt
      421 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
      481 gtcctaatgc cccggtayaa accagcatgt caacctgtgg tctgtgtggg tcctaatgcc
      541 ccggtataaa ccagcagatc aacctgtggt ctgtgtgggt cctaatgccc cagtataaac
      601 cagcagatca acctgtggtc tgtgtgggtc ctaawgtccc naggnataaa ccagcatgtc
      661 aacctgtggt ctgtgtgggt cctaatgccc cggtataaac cagcagatca acctgtggtc
      721 tgtgtgggtc ctaatgcccc aggataaacc agcaggtcaa cctgtggtct gtgtgggtcc
      781 taatgcccca gtataaacca gcaggtcaac ctgtggtctg tgtgggtcct aatgccccag
      841 tatattgatg gagactctat actgctcggt gagctccatc tttcggataa gatgttaaaa
      901 caaggtcccg actctctgtg gtggtaaaaa attcccagga tgtccttcct accctggcat
      961 cctggacaaa tttgctcact gtcctctgtc catcatggcc tcctaaccat ccccatatca
     1021 taattggctt tatcactcta gctggggccg aaatcgcata cttccatttt atgaggtacg
     1081 ctaaaatcag tatgcgagcc aagtaatatg tccgaatact tccggcgaga ttctagagtg
     1141 tgcattcgat gcacgctacg ctattccatg atgccccgtg agagaattct tgaatcggag
     1201 agaagcaacg caactcatgc gggtaggtta cgtgacgatg acatattggc ggatgtagta
     1261 cgtccaagtt ccattcatac tgctcacatt catattttat agaatgttct tttcccacag
     1321 ccaagtagta agtttaaatt caaatacagt acctactaag tagtagtaga cggtttcgga
     1381 cgcagcccct gtctcctctc caccaatata ctggtgtgta gtgtgcggtc tggtgcaata
     1441 tggctgccat cgcgtcatcc aggtggatgc tgcacactgg tggttgatga ggagattttg
     1501 cccaaaaaag tgttttgagt ttccagaaaa gcgcgatata aatgtaagga attatcatta
     1561 gcttattata atggaacttt ctggataact tgtgccagag ctaggggcca ttaaaacttt
     1621 gccctggggt acctcaggga gctgcaagga agtgctatag ggctaatgaa atgtttgggg
     1681 gaaaaaaaaa aattttataa gtaaaaataa aactaaaata attaataatt tcattgttga
     1741 aaattatagt aaaattgata aacaagataa gcagcattag tataaaaaac taaaaacaaa
     1801 cgtaatttaa aataattaaa ttgttttaaa taaattatac aaaataatgt ttacaacgac
     1861 acggtggtgc agtaggtagt gctgtcgcca gcaaagcaag aaggtcgctg gttcgagcct
     1921 cagctggatc acttgatgtt tctgtgtgga gcattcgcgt gcgtttaacc ccagattaat
     1981 aaagagacta agccgaaaag aaaataaatg tatgaaaatg tttacgtaaa atgtaaataa
     2041 atgcatgatt aataaaatat atgaatttta aagattgagg tagaattgtt ttattattca
     2101 cacatttggt tatttttaat cagtgacaac ttgtgatgct ctttatattg tttattacgc
     2161 tactttgaat attcagtctc aaattgcatg taaatacgac ttcaaagcag aattagttta
     2221 atttaattgc ctgtaaacag tgttgtactt tgatggtgat tgtctgataa taatatgatt
     2281 tcactattta aaatttgaaa aaaaaaaatc tgaaaaatac tcaccggcca ctttattagg
     2341 tacacctgtc taactgcttg ttaacgtaaa tttctaatca gccaatcacc aggcagcaac
     2401 tcagtgcatt taggcatgta gacatggtca agacgatctg ctgcagttca aagcgagcat
     2461 cagaatggag aagcaaggtg atttaagtga ctttaaacgt gacgtgtttg ttggtgccag
     2521 acgggctggt ctgagtattt cagaaactgc tgatttactg gggttttcac acacaaacat
     2581 ctctggggtt tacagagaat ggtccaaaaa aagataatat ccagtgagcg gcagttctgt
     2641 gggggcaaat gtcttgttga tgccagaggt cagaggagaa tggcctgact ggttccagct
     2701 gatagaaagg caacagtaat tcaaataacc actcgttaga actgaggcat gcagaagagc
     2761 acctttgaac acaacatgtc gaaccttgag gcgggtgggc tacagcagct caagaccaca
     2821 tcgggtgcca ctcctgtcag ctaagaacag gaaactgagg ctacaattcg cacaggctca
     2881 acataagtag acaatagaag attgaacatt atgttgcctg gtcagatgga tacattctac
     2941 cttgtatcaa cagttcaggc tggtgatggt ggtgtaatgg tgtgggggat atattcttgg
     3001 cacactttgg gcccattagt accaattaac catcgtgtca acgccacagc ctaactgagt
     3061 attgttgctg accatgttca tttctttttg accacagtgt acccatcttc tgatggctac
     3121 ttcctgcagg ataatgcacc atgtcataaa gcgtgaatga tctcagactg gtttcttgaa
     3181 catgacaatg agttcactgt gctcgaatga cctccacagt caccagatct caatccaata
     3241 gagcgccttt ggggtgtggt agaatggaag attcgcatcc tggatgtgca gccgacaaat
     3301 ctgcatgatg ctagcatgtc aatatggacc aaaatctctg aggaattttc cagtacctgg
     3361 ttgaaatgca aaaggaaggg aatctatgct aaggcaaaaa gaggtccaac ccagtactag
     3421 tggggtgtac ataataaagt ggccggtgag tgtatattgt taaggatatc tagataatcc
     3481 aaataattta cagcactatt taaaaaaaaa actttatgtc accaagtctg catttatttg
     3541 atcacaatta cagtcgaaaa agcaatatcg ggatacattg tttccattta aaataactat
     3601 attatatttt aaatattttt gacaatgtta tttctttaat gccaaagcta aattgtattc
     3661 aatctgaatt ttagtgtcac atgatccttc agaaaatatt aacacattgc taaaaatgta
     3721 tattattatc aatttggatt tttttttttt ttttgtatat taacctatgc tattggttac
     3781 tatacaaaag tattactgtg aatatatgca tatacagaac cgtatcaagc gcggggggtg
     3841 gttgggttat gtccccattt ggaaagacct ggaaacattc ataatgacta aaattgcacg
     3901 gaaaaatata atatctatga gtattgctga tcagcagcac aaaaccagaa ctccatatta
     3961 ataaacagcc aatgatgcag tgagtttgtg catgttttca ttcaaactcg tgtgattgtg
     4021 ctcaaacacc tgttgtgagc agcagttgca aacagtaaac ctcaagagga gccagtcaac
     4081 cttcacaaac actcaacctc tggctcactc actaaacaat tcgcgttaat atctaaaata
     4141 aaaatttcca gcaccaaaca gctctcagat ttcggagtac actgaccaag aggtaagaag
     4201 taacgttaca ttactaggac gactattgta agactagata tgtaatattt ggaagcgtac
     4261 gtgttttaag tgacatatag agtagaggaa ctgatggaaa tacgatttgc ataatgtaaa
     4321 atgatatata ttaaaatata ggctacagta ggtaacgtgg tttacatata acgttactgt
     4381 gttaatgtag tgctcagtta ctcgtgttga cagttattat tagtgcttgc taatttgttt
     4441 gttaatgctt ctcttgcgta tttgattacc ttcaggcgaa tctaaaacat atcagatgta
     4501 gaaacggcag aaaaatgttg aagaaaatac gagaatagta gcttttggag cacatagaaa
     4561 agtacatcag ataaagccta ccgtttaaac gcagacgccg ctctcgcact gaatgctggg
     4621 tactgtagtc cttatattgt atgcttaatg tgcgtctcaa cgggaaacta gagttaggaa
     4681 aactacaatt tcctgtggaa aatgtttctg ttttcgggga aacagcgaaa gaaagatatg
     4741 gttccgcgtt tttttcttac tttgaataat aaaataagac ctaactacac acttcgctta
     4801 ctcttatgct aatttgaagc actagcattc tttgatacaa tgataatcat ttaaacatca
     4861 cagttatact acaaatacta tggttaaact atgtctagtg tagtgtaaca ctatggttgc
     4921 cattgttatc tttaagttta agtattatct gtggtaaagc catggttggt ttttataaga
     4981 ggtaataaaa ttaacattgt acaattaaat gactataaat taatgttttt ttttattttt
     5041 tttacacagc taccttcaaa aagggatcag aaggggaacg aaagatggcg gcggaaacgc
     5101 tgctgtccag tttgttagga ctgctgcttc tgggactcct gttacccgca agtctgaccg
     5161 gcggtgtcgg gagcctgaac ctggaggagc tgagtgagat gcgttatggg atcgagatcc
     5221 tgccgttgcc tgtcatggga gggcagagcc aatcttcgga cgtggtgatt gtctcctcta
     5281 agtacaaaca gcgctatgag tgtcgcctgc cagctggagc tattcacttc cagcgtgaaa
     5341 gggaggagga aacacctgct taccaagggc ctgggatccc tgagttgttg agcccaatga
     5401 gagatgctcc ctgcttgctg aagacaaagg actggtggac atatgaattc tgttatggac
     5461 gccacatcca gcaataccac atggaagatt cagagatcaa aggtgaagtc ctctatctcg
     5521 gctactacca atcagccttc gactgggatg atgaaacagc caaggcctcc aagcagcatc
     5581 gtcttaaacg ctaccacagc cagacctatg gcaatgggtc caagtgcgac cttaatggga
     5641 ggccccggga ggccgaggtt cggttcctct gtgacgaggg tgcaggtatc tctggggact
     5701 acatcgatcg cgtggacgag cccttgtcct gctcttatgt gctgaccatt cgcactcctc
     5761 ggctctgccc ccaccctctc ctccggcccc cacccagtgc tgcaccgcag gccatcctct
     5821 gtcacccttc cctacagcct gaggagtaca tggcctacgt tcagaggcaa gccgactcaa
     5881 agcagtatgg agataaaatc atagaggagc tgcaagatct aggcccccaa gtgtggagtg
     5941 agaccaagtc tggggtggca ccccaaaaga tggcaggtgc gagcccgacc aaggatgaca
     6001 gtaaggactc agatttctgg aagatgctta atgagccaga ggaccaggcc ccaggagggg
     6061 aggaggtgcc ggctgaggag caggacccaa gccctgaggc agcagattca gcttctggtg
     6121 ctcccaatga ttttcagaac aacgtgcagg tcaaagtcat tcgaagccct gcggatttga
     6181 ttcgattcat agaggagctg aaaggtggaa caaaaaaggg gaagccaaat ataggccaag
     6241 agcagcctgt ggatgatgct gcagaagtcc ctcagaggga accagagaag gaaaggggtg
     6301 atccagaacg gcagagagag atggaagaag aggaggatga ggatgaggat gaggatgaag
     6361 atgaggatga acggcagtta ctgggagaat ttgagaagga actggaaggg atcctgcttc
     6421 cgtcagaccg agaccggctc cgttcggagg tgaaggctgg catggagcgg gaactggaaa
     6481 acatcatcca ggagacagag aaagagctgg acccagatgg gctgaagaag gagtcagagc
     6541 gggatcgggc aatgctggct ctcacatcca ctctcaacaa actcatcaaa agactggagg
     6601 aaaaacagag tccagagctg gtgaagaagc acaagaaaaa gagggttgtc cccaaaaagc
     6661 ctcccccatc accccaacct acagggaaaa ttgagatcaa aattgtccgc ccatgggctg
     6721 aagggactga agagggtgca cgttggctga ctgatgagga cacgagaaac ctcaaggaga
     6781 tcttcttcaa tatcttggtg ccgggagctg aagaggccca gaaggaacgc cagcggcaga
     6841 aagagctgga gagcaattac cgccgggtgt ggggctctcc aggtggggag ggcacagggg
     6901 acctggacga atttgacttc tgagaccaac actacacttg acccttcacg gaatccagac
     6961 tcttcctgga cggcgatgat gatccagaca tgataagata cattgatgag tttggacaaa
     7021 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     7081 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattcg atgatgatcc
     7141 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7201 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7261 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7321 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     7381 agatcagatc tcttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7501 aatgtatctt atcatgtctg gatctacgta atacgactca ctatagttct agaggctcga
     7561 gaggggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     7621 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     7681 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     7741 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
     7801 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
     7861 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     7921 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     7981 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     8041 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     8101 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     8161 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     8221 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcttcgccc
     8281 ttgctcacca tggtcactgt ctgctttgct gttggtctgg gctcctgggt cactggctta
     8341 ctaatggagt ctttatgtat gaggactctt atcaattgtt cttctataaa ggtctgcagt
     8401 gtttctgttc gtcccctaca tggacaccca gagcctccta aatacaggag ccctgataac
     8461 tgcacaagtg ctcagattcc agcagggtgg aaatgagata aagtgtgcag atggggaggg
     8521 ggacgtgaat gagagatttg agggatgaaa aggatggatg aacgcattga aaatagcccc
     8581 tttcacaaaa taataccagt aaattgtcat ataattaatg accagacctt tacctgtaaa
     8641 tgatgtgatg ttcatataca gaagaatgcc aacaatttac agatgatttt acaaattctt
     8701 aattttcctt ttctaaatgg atttgttcac acattatctc ttcaaagcaa ttgaattttc
     8761 tggaaaagat tggctgtgtg tgtaaaaggt gctactgagg aatttaatgt gacatggaat
     8821 gaagcaaaca gcagcctaag acaggatggc aggaaaaatg tcacatgttt aaaataagag
     8881 tgaaaccaac gacctggact tatagagact gtgtgtcctt aggcaaagct cttgtacaaa
     8941 cagtgcttgt gactgtgaaa ttcggggttt gcctggattg tgttaaatgt gtgcttggat
     9001 gtatcataaa aaagaatcgg ttctctgtat ttgacatttt ctgtaattta tctaaagtta
     9061 caactggcta tgcctgattt ttatcactat tgaatcaatc attttaaaga atgttaagtc
     9121 tgctgatttg gagagctcat ttacatttat tttgaatgtc tcttatttaa cacaactgat
     9181 ttaagcttta gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg
     9241 gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt
     9301 gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg
     9361 acatgattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     9421 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc agatccacta
     9481 gttctagagc ggccattacc ctgttatccc taggccgcca ccgcggtgga gctccaattc
     9541 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     9601 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9661 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9721 cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     9781 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     9841 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     9901 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     9961 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    10021 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    10081 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    10141 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
    10201 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10261 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10321 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10381 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10441 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10501 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10561 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10621 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10681 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10741 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10801 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10861 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10921 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10981 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11041 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11101 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11161 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    11221 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    11281 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    11341 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    11401 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    11461 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    11521 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11581 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    11641 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    11701 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    11761 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11821 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11881 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11941 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    12001 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    12061 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    12121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    12181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    12241 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    12301 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
    12361 aaccctcact aaagggaaca aaagctgggt accgggcccc ccctcgaatt accctgttat
    12421 ccctatcgag caaaatcaat cctaatgtaa aatagtatac gttcatcatt ataatgcaaa
    12481 cactgtagaa ctgttgtgtt gtgccttgca tttcattctc agtttcttat cgtctgtctc
    12541 tttttacgac agagctagaa aaaactagcc ttcatttgcc atcgccgtat gcaaatccac
    12601 tccagaactc aacaggtttg ttctgcggcg ggtgtaagtg tgaacaccgg catgtaagtg
    12661 cgcttgagca ggagaataaa gcgctcagag acaccggagg aatcctttat tttgcgttgc
    12721 gcctcagggg attcgctgag ccttctcttc atggccctgc tcttcagaca caggtgctcc
    12781 gctgtttgtg tttttcactg ttcttcaggc actcgtggtt acgggttttt gttttgcact
    12841 cttgcacaca catctctcgg cctccaaaac aaacacacaa ctgcacgagg gctcgaagcc
    12901 cccaccgaaa aacaaaaaca ttatgctaat gaggaaggca cagctgttcc cttcttcact
    12961 ctctctctcg ttctttcaac aacagtcgcc taatttctct cgttccttta aagagccgcc
    13021 aagcagaaac agttaatgca aatataagcc tgcgggaaat aaggagaatt tttacaaaat
    13081 ccgagcttcc cctcctgcca aaatgtgatg tgtgactcac gcattcgata tttggtgtaa
    13141 tgagagtgaa tcattctgct ccacaccgaa cccgatgcca cagaccactc agatgcacac
    13201 cgaggagact attttaacag cacattacat ccagactgtc acagatgcat taccaagagt
    13261 gtgatttcag tgtcgaatgc aggtcttaaa ggaaaatctg ttgcgattgc atgtgcgcta
    13321 actagacacg accaacgaat gggaacaatc tgtgaaatat tatacaatca caggcaatta
    13381 aaagatgttt aaagttcaat ttagttttaa ttaatatgtt atataaaact aatctgtttt
    13441 aaatgtatat ttatgatttt agctttagct agcctcttga ccagcagctg aagtgtccgg
    13501 caagtaccta aaagggagac cacatgggaa agctaggttg ctcccggaag aggtgttagt
    13561 aaaccagcag gtctacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg
    13621 tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcaggt ctacctgtgg
    13681 tctgtgtggg tcctaatgcc ccagtataaa ccagcaggtc aacctgtagt ctgtgtgggt
    13741 cctaatgccc cagtataaac cagcaggtct acctgtggtc tgtgtgggtc ctaatgcccc
    13801 agtataaacc aacaggtcaa cctgtgatct gtgtgggtcc taatgcccca gtataaacca
    13861 gcaggtcaac ctgtggtctg tgtgggtcct aatgccccag tataaaccag caggtcaacc
    13921 tgtggtctgt gtgggtccta atgccccagt ataaaccagc aggtcaacgt gtggtctgtg
    13981 tgggtcctaa tgccctggta taaaccagca ggtcaacttg tggtctgtgt gggtcctaat
    14041 gccccggtat aaaccagcat gtcaacctgt ggtctgtgtg ggtcctaatg ccccggtata
    14101 aaccagcagr tcaacctgtg gtctgtgtgg gtcctaatgc cccagtataa accagcasgt
    14161 caaccygygt ycykykyggg tcctwatkmc scssyakmwt caaycamgyc aamcygwcsk
    14221 cykgtgtggk tcytartscc mtkcwnwaww ysryctstkt ggcctgttga tgtcgtaaga
    14281 tatgtatacg agacagcctg tggtctgtgt gggtcctaat gccccagtat aaaccagcag
    14341 gtcaacgtgt ggtctgtgtg ggtcctaatg ccctggtata aaccagcagg tcaacttgtg
    14401 gtctgtgtgg gtcctaatgc cccggtataa accagcatgt caacctgtgg tctgtttggt
    14461 tactaatgcc ccggtataaa ccagcacatc accctgtggt ctgggtgggt cctaatgcca
    14521 cagtataaac cagcaggcca acctgtggtc tgtgtggttc ctaatgcctc ggtacaaacc
    14581 agaatgccac catgaggtct ttccgggtcc taatgccaca gtataaatga cacggaggga
    14641 gaaagagcag aggtctaagc ctgcaggttc tctgttggtc attgcatggc ccaagacctc
    14701 ggtataaacc agtatgtcaa tctgtggttt gtgttggtcc taacaccgag gtatacacat
    14761 gtatgtccgt cggcagtgtg tgatgtttat aagacccagg tataaaccag caggtcaacc
    14821 tgtggtctgt gtgggtccta atgccccggt ataaaccagc atgtcaacct gtggtc
//
