LOCUS       sequence-44844-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-Flag-Kdrl-5p-FokI-EL
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..40
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             41..106
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gcggactgcc accatggctc caaagaagaa gcgtaaggta gactacaaag accatgacgg
       61 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagggta cccgcccata
      121 tgcttgccct gtcgagtcct gtgaccgccg cttcagccag aaggggcacc tcacccgcca
      181 tatccggatt cacacaggcc agaagccctt ccagtgtcgc atctgcatga ggaacttcag
      241 ccgcagcgac gcgctcaccc gccacatccg cacccacaca ggcgagaagc cttttgcatg
      301 cgacatctgc ggcaggaagt tcgcgcgcag cgacgcgctc aggaagcaca ccaaaatcca
      361 tacaggtgga tcccaactag tcaaaagtga actggaggag aagaaatctg aacttcgtca
      421 taaattgaaa tatgtgcctc atgaatatat tgaattaatt gaaattgcca gaaattccac
      481 tcaggataga attcttgaaa tgaaggtaat ggaatttttt atgaaagttt atggatatag
      541 aggtaaacat ttgggtggat caaggaaacc ggacggagca atttatactg tcggatctcc
      601 tattgattac ggtgtgatcg tggatactaa agcttatagc ggaggttata atctgccaat
      661 tggccaagca gatgaaatgg aacgatatgt cgaagaaaat caaacacgaa acaaacatct
      721 caaccctaat gaatggtgga aagtctatcc atcttctgta acggaattta agtttttatt
      781 tgtgagtggt cactttaaag gaaactacaa agctcagctt acacgattaa atcatatcac
      841 taattgtaat ggagctgttc
//
