LOCUS       sequence-448446-       11010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homology directed repair template for knocking in tdTomato reporter 
            to DDX4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pHDR_DDX4-T2A-tdTomato-puroTK
FEATURES             Location/Qualifiers
     source          1..11010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1..17)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(54..74)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(54..72)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(93..109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(93..109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(106..128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    117..133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(141..147,148..165,166..171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(324..341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(495..1083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(575..594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1254..2045,2046..2114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1877..1896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2115..2219)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2245..2700)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2382..2403)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2594..2613
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2827..2849
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2841..2858
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2842..2858
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2868..2887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2868..2886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4400..4453
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4454..5884
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    5885..5916
                     /label=rox
                     /bound_moiety="Dre recombinase"
     promoter        5966..6465
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6001..6024)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6398..6417
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6479..6488
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6485..7084
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6984..7004
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7091..8086
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(8108..8125)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8114..8338
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8386..8417
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             10020..10592
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          10669..10734
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
       61 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
      121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      301 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      901 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      961 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1021 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1081 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1141 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1201 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1261 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1321 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1381 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1441 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1501 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1561 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1621 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1681 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1741 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1801 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1861 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1921 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1981 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2041 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2101 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2161 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2221 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     2281 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2341 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2401 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2461 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2521 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2581 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     2641 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     2701 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     2761 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     2821 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
     2881 tatagggcga attgggtacc gggccccccc tcgagcgaat tgggcgtact ggtcgttgtg
     2941 ggaatactgg cagagcaatt tccttttttg atcttgaatc ggataaccat ttagcacagc
     3001 ctctagtaaa agtattgaca gatgtaagtt aaacttttat gatggaatgg atagttttct
     3061 tactttgttg ttgaaagtag acattttatg catgtgtata taacaagtaa acttttccaa
     3121 tatttaatac tttatgttgc atatgaagtc aatttttttt aaaggctcaa caggatgttc
     3181 ctgcatggtt ggaagaaatt gcctttagta catacattcc tggcttcagt ggtagtacaa
     3241 gaggaaacgt gtttgcatca gttgatacca gaaaggttag tagaaaggaa aacttgagaa
     3301 cttgtcttct agttactctt tgtaaatgtt gtgcttaata ttgtgaagta actataatat
     3361 ttaataatca agccattgtt ttcgtgcctg gaaggtagct actttattat ttcattaaca
     3421 ttttatgtat atacatattt ttggttttaa aggtcaggaa ttaactttga gatttatgcc
     3481 taaacagtct cctttcccag attttaaatt ttaatttgga atcttttaaa cttctgatgg
     3541 aattatttaa tttttgaatt ctgacaacat taattaatat aaaatacagg acctgttggg
     3601 gttacaagtc ttttcttttt cttttctttt tttttttttt tgagatagtg tctggctctg
     3661 ttgtccaggc tggagtgcag tggcatgatc ttggctcact gcaacctccg cctcccaggt
     3721 tcaaacaatt ctcatgcctc agcctccagt gtatctggga ttacaggcat gtgccaccat
     3781 cttagctggt tttttttttt tttttttnng tatttttaat agagacaggg tttcaccatg
     3841 ttggccaggc tggtctcaaa ctcctggcct caagtgatcc acctgcttca acctcccaaa
     3901 gtgctgagat tacaggcgtg agccactgcg cccagccacg aggctacaag tcttttctgg
     3961 tatgtcaggt ttttataggc tgaacaccgc ctttattcag aaacaatgag catgttaatt
     4021 aaatatttat gctgattcat tgagtgtagt gcattaagtt ttccccactt gtcaaaacta
     4081 ttgacttaaa aaatattttt cttgaaacat tttgtgcaat ttagatttcc tcatttgaca
     4141 ggagcctttt ttcaggtgaa tcagatttag tgagaataaa gttctttgat tatttgggga
     4201 agacatggta gatactttga gtccttgctc tcagtctgag tgatgtaact ttaaagctgt
     4261 cataaaatta aatgtttgtt tgtttctttt tttttttttt ttaaataatt accagggcaa
     4321 gagcactttg aacacagctg ggttttcttc ttcacaagct cccaatccag tagatgatga
     4381 gtcatgggat ggctctggcg agggcagagg aagtctgcta acatgcggtg acgtcgagga
     4441 gaatcctggc ccaatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
     4501 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4561 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     4621 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
     4681 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
     4741 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
     4801 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     4861 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     4921 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
     4981 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
     5041 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5101 gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg
     5161 cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag agttcatgcg
     5221 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     5281 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     5341 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     5401 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     5461 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     5521 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     5581 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     5641 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     5701 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     5761 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     5821 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
     5881 gtaataactt taaataatgc caattattta aagttacata tgagaaagta taggaacttc
     5941 agcttgatat cgaattaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     6001 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     6061 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     6121 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     6181 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     6241 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     6301 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     6361 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     6421 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaacgc
     6481 caccatgggg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6541 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6601 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6661 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6721 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6781 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6841 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6901 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6961 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7021 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7081 tgccggatcc atgcccacgc tactgcgggt ttatatagac ggtcctcacg ggatggggaa
     7141 aaccaccacc acgcaactgc tggtggccct gggttcgcgc gacgatatcg tctacgtacc
     7201 cgagccgatg acttactggc aggtgctggg ggcttccgag acaatcgcga acatctacac
     7261 cacacaacac cgcctcgacc agggtgagat atcggccggg gacgcggcgg tggtaatgac
     7321 aagcgcccag ataacaatgg gcatgcctta tgccgtgacc gacgccgttc tggctcctca
     7381 tatcgggggg gaggctggga gctcacatgc cccgcccccg gccctcaccc tcatcttcga
     7441 ccgccatccc atcgccgccc tcctgtgcta cccggccgcg cgatacctta tgggcagcat
     7501 gaccccccag gccgtgctgg cgttcgtggc cctcatcccg ccgaccttgc ccggcacaaa
     7561 catcgtgttg ggggcccttc cggaggacag acacatcgac cgcctggcca aacgccagcg
     7621 ccccggcgag cggcttgacc tggctatgct ggccgcgatt cgccgcgttt acgggctgct
     7681 tgccaatacg gtgcggtatc tgcagggcgg cgggtcgtgg cgggaggatt ggggacagct
     7741 ttcggggacg gccgtgccgc cccagggtgc cgagccccag agcaacgcgg gcccacgacc
     7801 ccatatcggg gacacgttat ttaccctgtt tcgggccccc gagttgctgg cccccaacgg
     7861 cgacctgtac aacgtgtttg cctgggcctt ggacgtcttg gccaaacgcc tccgtcccat
     7921 gcacgtcttt atcctggatt acgaccaatc gcccgccggc tgccgggacg ccctgctgca
     7981 acttacctcc gggatggtcc agacccacgt caccaccccc ggctccatac cgacgatctg
     8041 cgacctggcg cgcacgtttg cccgggagat gggggaggct aactgagctc tagagctcgc
     8101 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     8161 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     8221 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     8281 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     8341 tctgaggcgg aaagaaccag ctggggctcg acatactaga gtacttaact ttaaataatg
     8401 ccaattattt aaagttaggc gcgccgatat ctagacccag ctagtctgtg gttttgatgc
     8461 agagaagaaa atagttttga tttttgagtt tttaacagaa gtataaaact taacattctc
     8521 atagctcctg tccttgtatt ctcactccta cacttaaaaa aaaaatcctt actgactagt
     8581 tatgtgagat gctaaaactt acaacattgc agttactgat acaaatggtg ttaactggga
     8641 atattaaagc attctaaatg tctttcttat ttctggtata ttctttaggg ggcttagaca
     8701 tgtttaatgt ttaaatgcca agtcttacta tagtgtttat tgatcttata aaacaagcaa
     8761 ataggatatg atacactttt ggttaaaaat tactgggtct catttttact tgagtcttta
     8821 aaacagtagt gtgtcactat aatgtgataa tctttaagag aaagtagaat acttaagcct
     8881 ttcaaagtga ttttgatttt tagatcatca gatgtatgat gaaaatggtt aaatgtttgt
     8941 gatgggagct ctgtactcaa tggcataaca atgtttattt ttataatata caatctttcc
     9001 ttgaaataaa ggatgaaaca cttttccctt aagttttcca tcagcacttt aaatttttct
     9061 ttgttgcata tcctagccca aactcctata tttttgaaac tgtttttttg tgtcttgcgt
     9121 aaccagtgca gtgaacaaag tcttacataa attaaatatt aatgtataaa cattactgca
     9181 cagatgtggc aatgaaaatc aatcttagaa ctcaaacccg tttatcaaca aaaaacaatg
     9241 ctgcttactc tttccataag tttttgattc atattttaag attgatgaaa gaaccctgtc
     9301 tttcttcatt ttccattata tatcttgaac ttactatgcc tagaataatg aagttctttt
     9361 tttttttgag acggagtctt gctcttgttg ctaaggctgg agtgtaatgg cgtgatcttg
     9421 gctcactgca acctctgcct cccgggttca agcagttctc ctgcctcagc ctctcccgag
     9481 tagctgggat tacagacacg cgccacaatg cccagctaat ttttagtaga gacgggtttc
     9541 actatgttgg ccagcctggt ctcgaactcc tgacctcgtg atccgcccgc ctccacctct
     9601 caaagtgctg ggattacacg cgtgagccac cgcacccagc ccgaagttat taattaattg
     9661 ttaaatgaac gaacccaaat atttaagacc cagtgaggtg taaatactgg ggagacaacc
     9721 tccagatctt gtcagtgtgg ttttcaagag gaagcaaaaa gcctctccac ccaggcctgg
     9781 aatgtttcca cccaatgtcg agcagtgtgg ttttgcaaga ggaagcaaaa agcctctcca
     9841 cccaggcctg gaatgtttcc acccaatgtc gagcaaaccc cgcccagcgt cttgtcattg
     9901 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
     9961 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca ggtcctcgcc atggatcctg
    10021 atgatgttgt tgattcttct aaatcttttg tgatggaaaa cttttcttcg taccacggga
    10081 ctaaacctgg ttatgtagat tccattcaaa aaggtataca aaagccaaaa tctggtacac
    10141 aaggaaatta tgacgatgat tggaaagggt tttatagtac cgacaataaa tacgacgctg
    10201 cgggatactc tgtagataat gaaaacccgc tctctggaaa agctggaggc gtggtcaaag
    10261 tgacgtatcc aggactgacg aaggttctcg cactaaaagt ggataatgcc gaaactatta
    10321 agaaagagtt aggtttaagt ctcactgaac cgttgatgga gcaagtcgga acggaagagt
    10381 ttatcaaaag gttcggtgat ggtgcttcgc gtgtagtgct cagccttccc ttcgctgagg
    10441 ggagttctag cgttgaatat attaataact gggaacaggc gaaagcgtta agcgtagaac
    10501 ttgagattaa ttttgaaacc cgtggaaaac gtggccaaga tgcgatgtat gagtatatgg
    10561 ctcaagcctg tgcaggaaat cgtgtcaggc gatctctttg tgaaggaacc ttacttctgt
    10621 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    10681 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    10741 acctatggaa ctgatgaatg ggagcagtgg tggaatgcag atccactaga gtcgagggat
    10801 gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt
    10861 catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc
    10921 tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga aggctcttta
    10981 ctattgcttt atgataatgt ttcatagttg
//
