LOCUS       sequence-448447-        8509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses histone deacetylase 10 (HDAC10) humanized catalytic 
            domain, also known as hzHDAC10, in Escherichia coli cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Herbst-Gervasoni CJ, Steimbach RR, Morgen M, Miller AK, Christianson
            DW
  TITLE     Structural Basis for the Selective Inhibition of HDAC10, the 
            Cytosolic Polyamine Deacetylase.
  JOURNAL   ACS Chem Biol. 2020 Aug 21;15(8):2154-2163. doi: 
            10.1021/acschembio.0c00362. Epub 2020 Jul 23.
  PUBMED    32659072
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.0c00362"; journalName: "ACS Chem Biol"; date: 
            "2020-08-21- 21"; volume: "15"; issue: "8"; pages: "2154-2163"
COMMENT     Sequence Label: hzHDAC10
FEATURES             Location/Qualifiers
     source          1..8509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     380..403
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             419..439
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2552..2569
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2622..2640)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2636..2683
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2720..3175
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2807..2826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3017..3038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3268..4083)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3268..4083)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3991..4010
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4075..4094
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4205..4793
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4694..4713
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4947..4964
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4979..5118
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5044..5063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5204..5226
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5220..5411)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6220..7302)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7264..7283
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7303..7380)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7586..7605
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7689..7708
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7689..7707
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7708..7732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7747..7769
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7788..7805
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(7815..8509,1..406)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
ORIGIN
        1 atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa
       61 gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc
      121 gaacaaagag ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga
      181 agcggttaat aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt
      241 ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc cagaaaggtg aaatcatgcc
      301 gaacatcccg cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc
      361 cagcggtcgt cagactgtcg atgaagccct gaaagacgcg cagactaatg ggatcgagga
      421 aaacctgtac ttccaatcca atgcaggtgg cagtgcagcg agcggtagcg cgctgatttt
      481 tgatgaggag atgagccgtt acaagctgct gtggaccgat ccggagtgcg agattgaggt
      541 tccggaacgt ctgaccgtga gctacgaagc gctgcgtacc catggtctgg cgcagcgttg
      601 caaagcggtg ccggttcgtc aggcgaccga gcaagaaatc ctgctggcgc acagcgagga
      661 atacctggaa gcggtgaaac aaaccccggg catgaacgtt gaggaactga tggcgttcag
      721 caagaaatac aacgccgttt attttcacca gaacatttat cactgcgcga aactggcggc
      781 gggtgcgacc ctgcaactgg tggatagcgt tatgaaacgt gaggtgcgca acggtatggc
      841 gctggttcgt ccgccgggtc accacagcca gcgtagcgcg gcgaacggtt tctgcgtgtt
      901 taacaacgtt gcgttcgcgg cgctgtacgc gaagaaaaac tataacctga accgtatcct
      961 gattgtggac tgggatgttc accacggtca gggcatccaa tactgcttcg aggaagaccc
     1021 gagcgtgctg tactttagct ggcaccgtta tgaacaccag agcttctggc cgaacctgcc
     1081 ggagagcgat tatagcagcg tgggcaaggg caaaggtagc ggctttaaca ttaacctgcc
     1141 gtggaacaag gttggcatga ccaacagcga ctacctggcg gcgttctttc acgtgctgct
     1201 gccggttgcg tatgaattcg atccggagct ggtgatcgtt agcgcgggtt ttgacagcgc
     1261 gattggtgat ccggagggcg aaatgtgcgc gctgccggaa atcttcgcgc acctgaccca
     1321 cctgctgatg ccgctggcgg cgggtaaaat gtgcgtggtt ctggagggtg gctacaacct
     1381 gaccagcctg ggtcagagcg tgtgccaaac cgtgcatagc ctgctgggcg acccgacccc
     1441 gcgtatcagc ggtctgggca ccgcgtgcga tagcgcgctg gaaagcattc agaacgtgcg
     1501 taacgttcaa agcagctatt ggagcagctt taagcacctg gcgcagagcg agaccaaccc
     1561 gaaacgtccg cgtctggatg cgaccaacgg tggcccgaag gagagcagcg aaccggcgag
     1621 cgaaagcaac ccgaagaaaa ccgcgcaaga tatcgtttgg ccggaaccgc tgaagcgtat
     1681 gccggcgagc gtgcgtaccg tggtggtgcc gccgccgggt gtggaactga ccctgccgaa
     1741 aaactgccag cacagcggcg acatcagcga aagcaccgcg aaagaggtgc agcgtattcg
     1801 tgataaacac ttccacgacc tgaccgatca aaacattctg cgtagcctgg gcaacatcat
     1861 tagcgtgctg gaccgtatga tgcgtagcga tgaagtttgc aacggttgcg ttgtggttag
     1921 cgacctgagc gtgagcgttc agtgcgcgct gcagcatgcg ctgaccgagc cggcggaacg
     1981 tgttctggtg gtgtacgttg gtgacggcga gctgccggtg aagaccaacg atggcaaagt
     2041 gtttctggtt cagatctgca ccaaggagac cgaagataaa tgcgttaacc gtctgaccct
     2101 gtgcctgcgt gagggtgaaa gcctgaccgc gggcttcatg caagcgctgc tgggtctgat
     2161 cctgccggtg gcgtacgaat ttaacccggc gctggtgctg ggcattgttg aggaaaccgc
     2221 ggcgaagacc cgtctgatgc gtgtttgggg tcacatgacc tgcctgatcc agggtctggc
     2281 gcgtggtcgt atgctgaccc tgctgcaagg ttatgacaaa gatctgctgg aactgaccgt
     2341 gagcgcgctg agcggtgcga gcattagccc gctgggtccg ctgcgtgcgc cgaagccgga
     2401 ggacgttgaa atgatggaga aacaacgcca acgcctgcaa gaacgctggg gtctgctgcg
     2461 ctgcaccgtt agcgaaagct ggtaataaca ttggaagtgg ataacggatc cgaattcgag
     2521 cgccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
     2581 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     2641 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     2701 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2761 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2821 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2881 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2941 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3001 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3061 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3121 ctgatttaac aaaaatttaa cgcgaatttt aacaaactag taacgtttac aatttcaggt
     3181 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3241 aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg
     3301 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     3361 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     3421 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     3481 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     3541 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     3601 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     3661 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     3721 aacaatgttt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     3781 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     3841 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     3901 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     3961 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     4021 agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct
     4081 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa
     4141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4321 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4441 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4501 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4561 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4621 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4681 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4741 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4801 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4861 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4921 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4981 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
     5041 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     5101 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5161 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5221 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
     5281 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
     5341 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     5401 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
     5461 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     5521 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
     5581 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
     5641 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
     5701 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
     5761 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
     5821 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg
     5881 gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg
     5941 acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc
     6001 gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt
     6061 cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc
     6121 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     6181 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6241 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6301 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     6361 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     6421 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     6481 gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg
     6541 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgataccctc
     6601 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     6661 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     6721 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     6781 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     6841 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     6901 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     6961 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     7021 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     7081 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     7141 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     7201 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     7261 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     7321 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     7381 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     7441 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     7501 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     7561 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     7621 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
     7681 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     7741 aataattttg tttaacttta agaaggagat ataccatggg ttcttctcac catcaccatc
     7801 accatggttc ttctatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
     7861 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
     7921 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
     7981 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
     8041 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
     8101 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
     8161 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
     8221 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
     8281 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
     8341 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
     8401 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
     8461 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggc
//
