LOCUS       sequence-448448-       11852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NATURA gene encoding plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Tabaglio T, Agarwal T, Cher WY, Ow JR, Chew AK, Sun PYQ, Reddy 
            Gurrampati RS, Lu H, Naidu P, Ng HK, Le Guezennec X, Ng SY, 
            Lakshmanan M, Guccione E, Wee KB
  TITLE     Unveiling sequence-agnostic mixed-chemical modification patterns for
            splice-switching oligonucleotides using the NATURA platform.
  JOURNAL   Mol Ther Nucleic Acids. 2025 Jan 7;36(1):102422. doi: 
            10.1016/j.omtn.2024.102422. eCollection 2025 Mar 11.
  PUBMED    39926316
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2025-01-7"; volume: "36(1):102422. doi"; 
            pages: " 10.1016/j.omtn.2024.102422. eCollection 2025 Mar 11"
COMMENT     Sequence Label: pC2.4
FEATURES             Location/Qualifiers
     source          1..11852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1216..1239
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1252..1272
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2819..3514
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     CDS             join(3514..3516,3517..3519,3520..3522,3523..4236)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     CDS             join(3514..3516,3517..3519,3520..3522,3523..4233)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(3562..3583)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3823..3842)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4170..4191
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4470..4487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4499..6151
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4565..4583)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    6174..6198
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6205..6229)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6307..6326)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6372..6427
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6426..6445)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        6782..7161
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7162..7365
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7316..7336
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7392..7401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7398..7796
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(7834..7851)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7840..8064
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8414..8476
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8542..8561)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8543..8561)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8568..8585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8568..8584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8577..8599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8726..9181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8813..8832)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9023..9044
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9207..9311
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9312..9380,9381..10172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9530..9549)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10343..10931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10832..10851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11085..11102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11219..11240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11255..11260,11261..11278,11279..11285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11293..11309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11298..11320
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11317..11333
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11317..11333
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        11354..11372
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   11521..11555
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    11832..11852
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac aagtttgtac aaaaaagcag
     1201 gctggatcca ccatggatta caaggatgac gatgacaagg gggtacctgc cccaaaaaaa
     1261 aaacgcaaag tggaggaccc agtaccagga tctagaggta agtggcaagc ttttgggagg
     1321 aggcctcagt taagaggccc acaagtttag aatgcttagt tcttcagagt tgtcactagc
     1381 tgctagcaaa gcctcagtta ttgcccagct actgaatgta tgccagatgc ggggagacag
     1441 tttcttacca tgttggagtt tggcgaatct tgagagttat gcctgagact gatctttttt
     1501 tcaataggtt gctataacta cctgtacaga atgaaagctc tggactgcca tccgtgcctc
     1561 tggtaagagc tacctgaaag ggtcctttct aggctctctg ggatttacat gggcacagag
     1621 tggtaaacct ataatgtatg ttaggatttg tcctttcagc acttctctgg atattaagaa
     1681 ttacctagag ttttgtaatc actgaattat ttagccaaag gaaattctgt tttttctcca
     1741 aagaatctag ggagttttgt tcttcttctt attatttttt tttaagattt agttatttat
     1801 ttatttgaaa ggcagagaga gagaaaggtc ttccattcac tggttcactc tccagatggc
     1861 cgcaacagcc agagctgcac cgacctgaag ccaggagcta ggagtttctt ccaggtctcc
     1921 catgtgggtg caggggccca agcacttgga ccatcatctt ctgctttccc aggccatagc
     1981 agagaactgg atcggaagtg gagcagccgg gacttgaact ggtgtccata tggaatgctg
     2041 gcactgcaga ccagggcttt aacccgctgt gccacagcgc tggcccctgt tcttttcctg
     2101 agattgttag tttcatgatt gggtaaaggt aatttgttgt ggggtttggg tttttttgtt
     2161 ttgttttgtt ttgttttttt ttttaatttt atttgaaaga gttacacaga gagcagtctt
     2221 ccatccactg gttcactctc cagttggcca caatggccag agctgggccg atctgaagcc
     2281 aggaaccagt agcttcttcc aggtctccca tgcgagtgca aggggccaag ggcttgggcc
     2341 atcttctgct gctttcccag gccacagcag agagctggat cagaagtgga gcaggcagtg
     2401 actttacccg ctatgccata gcacagaccc tggcaatttg ttcttggaca aaaggctagt
     2461 ggtgtctggt tttccactaa gcctaagata gatggttctt gttggatttc ttcattctgt
     2521 taccacttat catatatgtt ctatgtctac caaaacatag tttatatcta aagccaggaa
     2581 ggttaagtag ggtggtgaaa aggatgttga cgctatccgt tatgcttggc atagcttaca
     2641 gttgtgcttc taccgctaag gccatttagg tacagccgca tacctttggt tttcttaatt
     2701 ccattagaat ttcactccaa ttcactcatt tcattagacc tatttctgag tttgggaaag
     2761 taactagaaa gaatggtaga agaggtatca ttgtgctctt ttcttttcag ccggtgtcat
     2821 gtccgagctg atcaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
     2881 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     2941 gaagatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag
     3001 cttcatgtac ggcagcaaag ccttcatcaa ccacacccag ggcatccccg acttcttcaa
     3061 gcagtccttc cccgagggct tcacatggga gagaatcacc acatacgaag acgggggcgt
     3121 gctgaccgct acccaggaca ccagcttcca gaacggctgc atcatctaca acgtcaagat
     3181 caacggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac gcggctggga
     3241 ggccaacacc gagatgctgt accccgccga cggcggcctg agaggccaca gccagatggc
     3301 cctgaagctc gtgggcgggg gctacctgca ctgctccttc aagaccacat acagatccaa
     3361 gaaacccgcc aagaacctca agatgcccgg cttccacttc gtggaccaca gactggaaag
     3421 aatcaaggag gccgacaaag agacctacgt cgagcagcac gagatggctg tggccaagta
     3481 ctgcgacctc cccagcaaac tggggcacag ataatgtccg tgagcaaggg cgaggagctg
     3541 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3601 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3661 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3721 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3781 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3841 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3901 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3961 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4021 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4081 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4141 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4201 gggatcactc tcggcatgga cgagctgtac aaataagggc ccgtgtccat ttaatattaa
     4261 ttcctgattg ttctgtttca ttaatcattc agatagataa agattcttag gtcaaaaaaa
     4321 aaaaaaaaca ggaaatcata cccctacggt tccatccaca caaacctaaa aataagcatt
     4381 ggcactaagt actcagggaa agaacactgt ttagatatgt cattgtgtat gtgttttttt
     4441 cctcccttct agcactggaa gaagtgtcgc atcatcacca tcaccactaa gcggccgcat
     4501 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac
     4561 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
     4621 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt
     4681 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
     4741 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
     4801 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc
     4861 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
     4921 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt
     4981 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
     5041 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc
     5101 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca
     5161 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
     5221 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
     5281 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa
     5341 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat
     5401 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa
     5461 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg
     5521 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     5581 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     5641 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta
     5701 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     5761 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct
     5821 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca
     5881 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc
     5941 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     6001 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     6061 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     6121 ggccaagaag ggcggaaaga tcgccgtgta atgtctgaga cgggatccag cctgcttttt
     6181 tgtacaagaa agctgggtgc tagcacccag ctttcttgta caaagtggtg atcctcaggt
     6241 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     6301 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     6361 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     6421 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     6481 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     6541 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     6601 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     6661 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     6721 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttcgcgt
     6781 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     6841 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     6901 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     6961 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     7021 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     7081 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     7141 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     7201 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     7261 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     7321 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     7381 gaacccactg cgccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     7441 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     7501 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     7561 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     7621 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     7681 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     7741 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaactcg
     7801 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7861 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7921 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7981 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     8041 ctggggatgc ggtgggctct atggctcgag ttaattaacg agagcataat attgatatgt
     8101 gccaaagttg tttctgactg actaataagt ataatttgtt tctattatgt ataggttaag
     8161 ctaattactt attttataat acaacatgac tgtttttaaa gtacaaaata agtttatttt
     8221 tgtaaaagag agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt
     8281 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     8341 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     8401 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     8461 gattatcttt ctagggttaa ataatagttt ctaatttttt tattattcag cctgctgtcg
     8521 tgaataccga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     8581 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8641 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8701 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8761 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8821 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8881 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8941 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9001 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9061 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9121 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9181 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9241 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9301 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9361 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9421 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9481 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9541 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9601 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9661 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9721 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9781 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     9841 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     9901 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     9961 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10021 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10081 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10141 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10201 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10261 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10741 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    10861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    10921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    10981 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11041 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11101 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11161 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11221 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11281 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11341 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtacct cgcgcgactt
    11401 ggtttgccat tctttagcgc gcgtcgcgtc acacagcttg gccacaatgt ggtttttgtc
    11461 aaacgaagat tctatgacgt gtttaaagtt taggtcgagt aaagcgcaaa tcttttttaa
    11521 ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt
    11581 tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc
    11641 ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt
    11701 gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact catacgataa
    11761 ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt
    11821 atagatatca tcaactttgt atagaaaagt tg
//
