LOCUS       sequence-448478-        2346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express epegRNA for FXR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Chen X, Fansler MM, Janjos U, Ule J, Mayr C
  TITLE     The FXR1 network acts as signaling scaffold for actomyosin 
            remodeling.
  JOURNAL   Cell. 5 August 2024. doi: 10.1016/j.cell.2024.07.015.
  PUBMED    37961296
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 5 
            August 2024. doi: 10.1016/j.cell.2024.07.015."
COMMENT     Sequence Label: pU6-tevopreq1-FXR1-G266E
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     31..50
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(269..373)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        480..728
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     480..500
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     651..670
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        750..825
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     rep_origin      complement(995..1583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1075..1094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1754..2346,1..199,200..268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
       61 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      121 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      181 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      241 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      301 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      361 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgctagc tgtacaaaaa
      421 agcaggcttt aaaggaacca attcagtcga ctggatccgg taccaaggtc gggcaggaag
      481 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      541 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      601 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      661 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      721 gaaacaccga ctggaacatt cagaatctag ttttagagct agaaatagca agttaaaata
      781 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcactta cctcttcgta
      841 gattctgaat gttccgcggt tctatctagt tacgcgttaa accaactaga atttttttaa
      901 gcttgggccg ctcgaggtac ctctctacat atgacatgtg agcaaaaggc cagcaaaagg
      961 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1021 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1081 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1141 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1201 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1261 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1321 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1381 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1441 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1501 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1561 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1621 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1681 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1741 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1801 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1861 taccatctgg ccccagtgct gcaatgatac cgcgagatcc acgctcaccg gctccagatt
     1921 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1981 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2041 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2101 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2161 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2221 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2281 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2341 ggcgac
//
