LOCUS       sequence-44848-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN41-R4R3
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     656..678
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(716..734)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
       61 cttcttgacg agttcttctg agctagccgg ccatacaagt aatccggatg atcgacgcca
      121 acgtcgttga attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt
      181 tattctcttt gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca
      241 tttttttttc tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt
      301 gcaagtttat ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg
      361 gggaaacaca caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc
      421 aattcagaaa tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga
      481 aaactagtcc aagatctcag tggtcctagg tgagctccca tatggtgcac tctcagtgca
      541 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
      601 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
      661 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
      721 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      781 ggcacttttc ggggaaatgt gcgcggaacc cctatttg
//
