LOCUS       sequence-44849-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN42-R4R3
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     779..801
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     793..810
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     794..810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccggggatcc acgagagcgt cggatatttt acctggaaat aaattatttt atgatgaaag
       61 ttaaatacaa taagatacaa cgggaaacgt aagaaaaatt caataaaatc aattgtacac
      121 gtaggttttc aagcaaattt aagaatacaa ttttacaaag tcctaaatgt catttttcct
      181 gctcaaactt ttgactgaaa tgcataaaaa ttcgctgata aaaacaagca gaatacgcca
      241 aaatagttgc aaattataaa atttacagaa ttaatacaaa aaattcgata ttttactcaa
      301 aaaaatggaa aaaaagaaga aaaataaatt aaactttcgt tgagctggcc taaaatctaa
      361 tagcaagcgc gctccattgc caaacggtgt aaacgcggag cctcgtttgc gtgtagtttt
      421 tatcgtaaat cgacacaact tctttcaaaa taaattattt cttatgagta attttctgca
      481 ttctattgtt gtgtttcaat taatcttatt tttaatttga ttttattcaa aattatataa
      541 ttttattaag atgctgggtt cttctctccg cattttctcc cctcgagggg ggcccggcat
      601 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
      661 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      721 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      781 cagtcacgac gttgtaaaac gacggccagt gaattatcaa ctatg
//
