LOCUS       sequence-44854-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN43-R4R3
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     362..380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(418..440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      301 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      361 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
      421 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
      481 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
      541 ggcatcagag cagattgcac tgagagtgca ccatatggga gctcacctag gaccactgag
      601 atcttggact agttttcctt cctcctctat atatttttat gttttctttt aaaccttcct
      661 ccatatttct gaattgaatg ttgttgcata ttgtgtgtcc ttatttcatt tccatcatcc
      721 ttttgtgtgt ttccc
//
