LOCUS       sequence-44857-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE0k-RFP
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             174..185
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(194..228)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     primer_bind     complement(434..453)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgacgcagcg gaccaccttt ggtaactttc agtttagcgg tctgggtacc ttcgtacgga
       61 cgaccttcac cttcaccttc gatttcgaac tcgtgaccgt taacggaacc ttccatacga
      121 actttgaaac gcatgaactc tttgataacg tcttcggagg aagccatcta gtatttctcc
      181 tctttcacta gtagctagca cagtacctag gactgagcta gctgtcaaag atctcatgaa
      241 ttcggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
      301 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcatgatctg atcaagagac
      361 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
      421 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
      481 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
      541 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
      601 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
      661 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
      721 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
      781 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
      841 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
      901 caaggcgcgc
//
