LOCUS       sequence-44858-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN44-R4R3
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     826..848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     840..857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     841..857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atccatcttg ttcaatcatg gtcggtacct ttttttctga gccaatcccg gggatccacg
       61 agagcgtcgg atattttacc tggaaataaa ttattttatg atgaaagtta aatacaataa
      121 gatacaacgg gaaacgtaag aaaaattcaa taaaatcaat tgtacacgta ggttttcaag
      181 caaatttaag aatacaattt tacaaagtcc taaatgtcat ttttcctgct caaacttttg
      241 actgaaatgc ataaaaattc gctgataaaa acaagcagaa tacgccaaaa tagttgcaaa
      301 ttataaaatt tacagaatta atacaaaaaa ttcgatattt tactcaaaaa aatggaaaaa
      361 aagaagaaaa ataaattaaa ctttcgttga gctggcctaa aatctaatag caagcgcgct
      421 ccattgccaa acggtgtaaa cgcggagcct cgtttgcgtg tagtttttat cgtaaatcga
      481 cacaacttct ttcaaaataa attatttctt atgagtaatt ttctgcattc tattgttgtg
      541 tttcaattaa tcttattttt aatttgattt tattcaaaat tatataattt tattaagatg
      601 ctgggttctt ctctccgcat tttctcccct cgaggggggc ccggcatatg cggtgtgaaa
      661 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
      721 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
      781 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
      841 gtaaaacgac ggccagtgaa
//
