LOCUS       sequence-44862-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Semple JI, Biondini L, Lehner B
  TITLE     Generating transgenic nematodes by bombardment and antibiotic 
            selection.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):118-9. doi: 10.1038/nmeth.1864.
  PUBMED    22290182
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "118-9"
COMMENT     Sequence Label: pBCN45-R4R3
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     645..667
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(705..723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
       61 gttcttctga gctagccggc catacaagta atccggatga tcgacgccaa cgtcgttgaa
      121 ttttcaaatt ttaaatactg aatatttgtt ttttttccta ttatttattt attctctttg
      181 tgtttttttt cttgctttct aaaaaattaa ttcaatccaa atctaaacat ttttttttct
      241 ctttccgtct cccaattcgt attccgctcc tctcatctga acacaatgtg caagtttatt
      301 tatcttctcg ctttcatttc attaggacgt ggggggaatt ggtggaaggg ggaaacacac
      361 aaaaggatga tggaaatgaa ataaggacac acaatatgca acaacattca attcagaaat
      421 atggaggaag gtttaaaaga aaacataaaa atatatagag gaggaaggaa aactagtcca
      481 agatctcagt ggtcctaggt gagctcccat atggtgcact ctcagtgcaa tctgctctga
      541 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      601 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      661 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      721 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      841 gctcatgaga caataaccc
//
