LOCUS       sequence-448633-       11585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Start codon reporter with mutated start codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 11585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pGFP-ACG-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..11585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          20..85
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             215..235
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    660..794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(697..716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     751..770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             962..1595
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1642..1767
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2264..2497
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2682..2726
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3011..3128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3870..4277
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4318..5031
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             4318..5028
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4345..4373)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4968..4988
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(5044..5618,5619..5621,5622..5630)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5211..5228)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5438..5457
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5643..6353
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5788..5806)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6056..6075
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6410..6998
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6463..6483)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6881..6892)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7001..7017)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7002..7018)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7108..7341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7366..7384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7367..7384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7674..7693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7907..7926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8026..8048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8086..8104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8172..8276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8277..8345,8346..9137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8495..8514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9308..9896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9797..9816
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10050..10067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10137..10157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10142..10471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10322..10457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
       61 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
      121 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
      181 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
      241 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
      301 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
      361 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
      421 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
      481 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
      541 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
      601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
      661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      781 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
      841 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
      901 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
      961 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     1021 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     1081 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     1141 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     1201 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     1261 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     1321 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     1381 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     1441 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     1501 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     1561 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     1621 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     1681 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     1741 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     1801 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     1861 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1921 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1981 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     2041 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     2101 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     2161 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     2221 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     2281 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     2341 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     2401 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2461 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2521 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2581 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2641 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2701 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2761 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2821 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2881 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     2941 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat
     3001 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3061 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3121 aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata
     3181 tcgaattcgg gaggtggtcc ctgcagttac gccaatgata acccccgcca gaaaaatctt
     3241 agtagccttc cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg
     3301 gaaacacccg aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg
     3361 cccgcgtctg tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca
     3421 aaggggtact agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca
     3481 ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg
     3541 ctgccaagca gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc
     3601 ccttccctcc ccttggggac caccgggcgc cacgccgcga acggtaagtg ccgcggtcgt
     3661 cggcgcctcc gccctccccc tagggcccca attcccagcg ggcgcggcgc gcggccccgc
     3721 gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggagtcctg
     3781 cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     3841 ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca tggaaaaata
     3901 ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag ctaacgttgg
     3961 gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa gaacagatgg
     4021 tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga tatggcccaa
     4081 ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg acctgaaatg
     4141 accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt cgcgcgcttc
     4201 tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca gtcctccgac
     4261 agactgagtc gcccgggggg gatctggagc tctcgagaat tctcagccgc caccacgagc
     4321 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     4381 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     4441 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     4501 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     4561 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     4621 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     4681 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     4741 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     4801 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     4861 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     4921 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     4981 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaata ataagcggcc
     5041 gcgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     5101 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     5161 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     5221 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     5281 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     5341 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     5401 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     5461 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     5521 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     5581 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atggcgtccg
     5641 gaatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     5701 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     5761 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     5821 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     5881 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     5941 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     6001 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     6061 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     6121 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     6181 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     6241 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6301 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taataagcga
     6361 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     6421 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6481 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6541 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6601 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6661 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6721 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6781 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6841 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6901 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6961 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
     7021 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     7081 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7141 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7201 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7261 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7321 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     7381 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     7441 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     7501 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     7561 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     7621 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     7681 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     7741 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     7801 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     7861 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
     7921 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     7981 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8041 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     8101 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     8161 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8221 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8281 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8341 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8401 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8461 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8521 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8581 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8641 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8701 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8761 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8821 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8881 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8941 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9001 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9061 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
    10141 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10201 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    10261 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    10321 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10381 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    10441 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
    10501 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
    10561 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
    10621 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
    10681 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
    10741 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
    10801 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
    10861 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
    10921 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
    10981 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
    11041 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
    11101 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
    11161 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
    11221 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
    11281 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
    11341 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
    11401 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
    11461 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
    11521 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
    11581 cctac
//
