LOCUS       sequence-448648-        9917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EIF3D variant overexpression vector with sgRNA targeting eIF3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pEIF3D-a5helixDel-sgEIF3D
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2814..2847)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2851..2871
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3116..3327
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3328..4266
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4223..4243
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4289..4987
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4997..5053
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(6717..6750)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6806..7394
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6859..6879)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7277..7288)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7397..7413)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7398..7414)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7923..8103
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8165..8753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8245..8264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8924..9715,9716..9784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9547..9566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9785..9889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
     2641 gcccgcgccg gccccctcgc acggacttgt gggagaagct cggctactcc cctgccccgg
     2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataactt
     2821 cgtatagcat acattatacg aagttataaa cagcacaaaa ggaaactcac cctaactgta
     2881 aagtaattgt gtgttttgag actataagta tcccttggag aaccaccttg ttggctgctg
     2941 ctaacgcggt cccgtttaag agctaagctg gaaacagcat agcaagttta aataaggcta
     3001 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg agtactagga
     3061 tccattaggc ggccgcgtgg ataaccgtat taccgccatg catgtgcccg tcagtgggca
     3121 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     3181 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     3241 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     3301 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     3361 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     3421 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     3481 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     3541 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     3601 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     3661 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     3721 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     3781 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     3841 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     3901 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3961 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     4021 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgcgctt ttggagtacg
     4081 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     4141 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     4201 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     4261 tttcaggtgt cgtgagctag ccgccaccat gagcgagctg attaaggaga acatgcacat
     4321 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     4381 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     4441 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     4501 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     4561 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
     4621 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     4681 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     4741 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     4801 cgcaaacatc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     4861 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     4921 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     4981 gcttaatggt agtggggcta ctaacttcag cctgctgaag caggctggag acgtggagga
     5041 gaaccctgga cctgcaaagt tcatgacacc cgtgatccag gacaacccat caggctgggg
     5101 tccctgtgcg gttcccgagc agtttcggga tatgccctac cagccgttca gcaaaggaga
     5161 tcggctagga aaggttgcag actggacagg agccacatac caagataaga ggtacacaaa
     5221 taagtactcc tctcagtttg gtggtggaag tcaatatgct tatttccatg aggaggatga
     5281 aagtagcttc cagctggtgg atacagcgcg cacacagaag acggcctacc agcggaatcg
     5341 aatgagattt gcccagagga acctccgcag agacaaagat cgtcggaaca tgttgcagtt
     5401 caacctgcag atcctgccta agagtgccaa acagaaagag agagaacgca ttcgactgca
     5461 gaaaaagttc cagaaacaat ttggggttag gcagaaatgg gatcagaaat cacagaaacc
     5521 ccgagactct tcagttgaag ttcgtagtga ttgggaagtg aaagaggaaa tggattttcc
     5581 tcagttgatg aagatgcgct acttggaagt atcagagcca caggacattg agtgttgtgg
     5641 ggccctagaa tactacgaca aagcctttga ccgcatcacc acgaggagtg agaagccact
     5701 gcggagcatc aagcgcatct tccacactgt caccaccaca gacgaccctg tcatccgcaa
     5761 gctggcaaaa actcagggga atgtgtttgc cactcaggcc atcctggcca cgctgatgag
     5821 ctgtacccgc tcaatcgctt cctgggatat tgtcgtccag agagttgggt ccaaactctt
     5881 ctttgacaag agagacaact ctgactttga cctcctgaca gtgagtgaga ctgccaatga
     5941 gccccctcaa gatgaaggta attccttcaa ttcaccccgc aacctggcca tggaggcaac
     6001 ctacatcaac cacaatttct cccagcagtg cttgagaatg gggaaggaaa gatacaactt
     6061 ccccaaccca aacccgtttg tggaggacga catggataag aatgaaatcg cctctgttgc
     6121 gtaccgttac cgcaggtgga agcttggaga tgatattgac cttattgtcc gttgtgagca
     6181 cgatggcgtc atgactggag ccaacgggga agtgtccttc atcaacatca agacactcaa
     6241 tgagtgggat tccaggcact gtaatggcgt tgactggcgt cagaagctgg actctcagcg
     6301 aggggctgtc attgccacgg agctgaagaa caacagctac aagttggccc ggtggacctg
     6361 ctgtgctttg ctggctggat ctgagtacct caagcttggt tatgtgtctc ggtaccacgt
     6421 gaaagactcc tcacgccacg tcatcctagg cacccagcag ttcaagccta atgagtttgc
     6481 cagccagatc aacctgagcg tggagaatgc ctggggcatt ttacgctgcg tcattgacat
     6541 ctgcatgaag ctggaggagg gcaaatacct catcctcaag gaccccaaca agcaggtcat
     6601 ccgtgtctac agcctccctg atggcacctt cagctctgat gaagatgagg aggaagagga
     6661 ggaggaagaa gaggaagaag aagaggaaga aacttaagaa ttcgtcgagg gacctaataa
     6721 cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt tccactaggt
     6781 acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     6841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7201 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7381 ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa
     7441 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     7501 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     7561 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7621 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     7681 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     7741 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     7801 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     7861 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     7921 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8101 gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8581 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8641 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8881 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9061 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9541 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9901 gaaaagtgcc acctgac
//
