LOCUS       sequence-448650-        9863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EIF3D variant overexpression vector with sgRNA targeting eIF3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pEIF3D-RNAgateDel-sgEIF3D
FEATURES             Location/Qualifiers
     source          1..9863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2813..2846)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2850..2870
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3194..3601
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3613..4209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3613..3632)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4109..4129
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4219..4272
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4285..4980
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             4990..5046
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(6662..6695)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6751..7339
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6804..6824)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7222..7233)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7342..7358)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7343..7359)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7868..8048
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8110..8698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8190..8209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8869..9660,9661..9729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9492..9511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9730..9834)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg
     2641 cccgcgccgg ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccccggt
     2701 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
     2761 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataacttc
     2821 gtatagcata cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa
     2881 agtaattgtg tgttttgaga ctataagtat cccttggaga accaccttgt tggctgctgc
     2941 taacgcggtc ccgtttaaga gctaagctgg aaacagcata gcaagtttaa ataaggctag
     3001 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctcga gtactaggat
     3061 ccattaggcg gccgcgtgga taaccgtatt accgccatgc attgcagccc cgataaaata
     3121 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     3181 caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac caagaataga
     3241 gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa caggatatct
     3301 gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg cagtttcggc
     3361 ccgggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca gcagtttctt
     3421 aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg cgccttattt
     3481 gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc ccgagctcta
     3541 taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg agtcgcccgg
     3601 ggctagccca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3661 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact acccagccac gcgccacacc
     3721 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3781 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     3841 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3901 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3961 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     4021 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     4081 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     4141 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     4201 cccggtgccg gatcgggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     4261 aatcctggcc caccggtcgc caccagcgag ctgattaagg agaacatgca catgaagctg
     4321 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     4381 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     4441 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     4501 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     4561 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     4621 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     4681 cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc
     4741 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     4801 atcaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     4861 tatgtggact acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag
     4921 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     4981 ggtagtgggg ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
     5041 ggacctgcaa agttcatgac acccgtgatc caggacaacc cctcgggctg gggtccctgt
     5101 gcggttcccg agcagtttcg ggatatgccc taccagccgt tcagcaaagg agatcggcta
     5161 ggaaaggttg cagactggac aggagccaca taccaagata agaggtacac aaataagtac
     5221 tcctctcagt ttggtggtgg aagtcaatat gcttatttcc atgaggagga tgaaagtagc
     5281 ttccagctgg tggatacagc gcgcacacag aagacggcct accagcggaa tcgaatgaga
     5341 tttgcccaga ggaacctccg cagagacaaa gatcgtcgga acatgttgca gttcaacctg
     5401 cagatcctgc ctaagagtgc caaacagaaa gagagagaac gcattcgact gcagaaaaag
     5461 ttccagaaac aatttggggt taggcagaaa tgggatcaga aatcacagaa accccgagac
     5521 tcttcagttg aagttcgtag tgattgggaa gtgaaagagg aaatggattt tcctcagttg
     5581 atgaagatgc gctacttgga agtatcagag ccacaggaca ttgagtgttg tggggcccta
     5641 gaatactacg acaaagcctt tgaccgcatc accacgagga gtgagaagcc actgcggagc
     5701 atcaagcgca tcttccacac tgtcaccacc acagacgacc ctgtcatccg caagctggca
     5761 aaaactcagg ggaatgtgtt tgccactgat gccatcctgg ccacgctgat gagctgtacc
     5821 cgctcagtgt attcctggga tattgtcgtc cagagagttg ggtccaaact cttctttgac
     5881 aagagagaca actctgactt tgacctcctg acaaattcac cccgcaaact ggccatggag
     5941 gcaacctaca tcaaccacaa tttctcccag cagtgcttga gaatgtggaa ggaaagatac
     6001 aacttcccca acccaaaccc gtttgtggag gacgacatgg ataagaatga aatcgcctct
     6061 gttgcgtacc gttaccgcag gtggaagctt ggagatgata ttgaccttat tgtccgttgt
     6121 gagcacgatg gcgtcatgac tggagccaac ggggaagtgt ccttcatcaa catcaagaca
     6181 ctcaatgagt gggattccag gcactgtaat ggcgttgact ggcgtcagaa gctggactct
     6241 cagcgagggg ctgtcattgc cacggagctg aagaacaaca gctacaagtt ggcccggtgg
     6301 acctgctgtg ctttgctggc tggatctgag tacctcaagc ttggttatgt gtctcggtac
     6361 cacgtgaaag actcctcacg ccacgtcatc ctaggcaccc agcagttcaa gcctaatgag
     6421 tttgccagcc agatcaacct gagcgtggag aatgcctggg gcattttacg ctgcgtcatt
     6481 gacatctgca tgaagctgga ggagggcaaa tacctcatcc tcaaggaccc caacaagcag
     6541 gtcatccgtg tctacagcct ccctgatggc accttcagct ctgatgaaga tgaggaggaa
     6601 gaggaggagg aagaagagga agaagaagag gaagaaactt aagaattcgt cgagggacct
     6661 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
     6721 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     6781 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6841 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6901 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6961 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7021 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7081 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7141 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7201 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7261 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7321 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     7381 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     7441 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     7501 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7561 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7621 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     7681 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     7741 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     7801 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     7861 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7921 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7981 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8041 ctctagcagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8521 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8761 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8821 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8881 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8941 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9001 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9061 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9121 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9181 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9241 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9301 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9361 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9421 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9481 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9541 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9601 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9661 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9721 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9781 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9841 tccccgaaaa gtgccacctg acg
//
