LOCUS       sequence-448686-        9917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EIF3D variant overexpression vector with sgRNA targeting eIF3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   She R, Luo J, Weissman JS
  TITLE     Translational fidelity screens in mammalian cells reveal eIF3 and 
            eIF4G2 as regulators of start codon selectivity.
  JOURNAL   Nucleic Acids Res. 2023 Jul 7;51(12):6355-6369. doi: 
            10.1093/nar/gkad329.
  PUBMED    37144468
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-7- 7"; volume: "51"; issue: 
            "12"; pages: "6355-6369"
COMMENT     Sequence Label: pEIF3D-a11helixDel-sgEIF3D
FEATURES             Location/Qualifiers
     source          1..9917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..232
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          233..1171
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1128..1148
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1194..1892
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             1902..1958
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(3622..3655)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3711..4299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3764..3784)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4182..4193)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4302..4318)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4303..4319)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4828..5008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(5070..5658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5150..5169)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5829..6620,6621..6689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6452..6471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6690..6794)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6869..6888)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7060..7439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7441..7639
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7594..7614
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7657..7837
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7884..8009
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8506..8739
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8924..8968
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9266..9383
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(9636..9669)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9673..9693
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ccatgcatgt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
      361 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
      421 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
      481 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
      541 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
      601 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
      661 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
      721 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
      781 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
      841 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
      901 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
      961 ttagttctcg cgcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     1021 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     1081 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     1141 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gctagccgcc accatgagcg
     1201 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     1261 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     1321 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     1381 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     1441 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     1501 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     1561 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     1621 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     1681 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     1741 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     1801 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     1861 acctccctag caaactgggg cacaagctta atggtagtgg ggctactaac ttcagcctgc
     1921 tgaagcaggc tggagacgtg gaggagaacc ctggacctgc aaagttcatg acacccgtga
     1981 tccaggacaa cccctcaggc tggggtccct gtgcggttcc cgagcagttt cgggatatgc
     2041 cctaccagcc gttcagcaaa ggagatcggc taggaaaggt tgcagactgg acaggagcca
     2101 cataccaaga taagaggtac acaaataagt actcctctca gtttggtggt ggaagtcaat
     2161 atgcttattt ccatgaggag gatgaaagta gcttccagct ggtggataca gcgcgcacac
     2221 agaagacggc ctaccagcgg aatcgaatga gatttgccca gaggaacctc cgcagagaca
     2281 aagatcgtcg gaacatgttg cagttcaacc tgcagatcct gcctaagagt gccaaacaga
     2341 aagagagaga acgcattcga ctgcagaaaa agttccagaa acaatttggg gttaggcaga
     2401 aatgggatca gaaatcacag aaaccccgag actcttcagt tgaagttcgt agtgattggg
     2461 aagtgaaaga ggaaatggat tttcctcagt tgatgaagat gcgctacttg gaagtatcag
     2521 agccacagga cattgagtgt tgtggggccc tagaatacta cgacaaagcc tttgaccgca
     2581 tcaccacgag gagtgagaag ccactgcgga gcatcaagcg catcttccac actgtcacca
     2641 ccacagacga ccctgtcatc cgcaagctgg caaaaactca ggggaatgtg tttgccactg
     2701 atgccatcct ggccacgctg atgagctgta cccgctcagt gtattcctgg gatattgtcg
     2761 tccagagagt tgggtccaaa ctcttctttg acaagagaga caactctgac tttgacctcc
     2821 tgacagtgag tgagactgcc aatgagcccc ctcaagatga aggtaattcc ttcaattcac
     2881 cccgcaacct ggccatggag gcagagtaca tcgaggccaa tttctcccag cagtgcttga
     2941 gaatggggaa ggaaagatac aacttcccca acccaaaccc gtttgtggag gacgacatgg
     3001 ataagaatga aatcgcctct gttgcgtacc gttaccgcag gtggaagctt ggagatgata
     3061 ttgaccttat tgtccgttgt gagcacgatg gcgtcatgac tggagccaac ggggaagtgt
     3121 ccttcatcaa catcaagaca ctcaatgagt gggattccag gcactgtaat ggcgttgact
     3181 ggcgtcagaa gctggactct cagcgagggg ctgtcattgc cacggagctg aagaacaaca
     3241 gctacaagtt ggcccggtgg acctgctgtg ctttgctggc tggatctgag tacctcaagc
     3301 ttggttatgt gtctcggtac cacgtgaaag actcctcacg ccacgtcatc ctaggcaccc
     3361 agcagttcaa gcctaatgag tttgccagcc agatcaacct gagcgtggag aatgcctggg
     3421 gcattttacg ctgcgtcatt gacatctgca tgaagctgga ggagggcaaa tacctcatcc
     3481 tcaaggaccc caacaagcag gtcatccgtg tctacagcct ccctgatggc accttcagct
     3541 ctgatgaaga tgaggaggaa gaggaggagg aagaagagga agaagaagag gaagaaactt
     3601 aagaattcgt cgagggacct aataacttcg tatagcatac attatacgaa gttatacatg
     3661 tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc
     3721 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3781 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3841 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     3901 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     3961 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4021 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4081 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4141 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4201 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4261 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat
     4321 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     4381 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     4441 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4501 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4561 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4621 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     4681 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     4741 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     4801 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     4861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4981 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     5041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5761 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5821 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5881 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5941 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6001 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6061 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6121 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6181 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6241 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6301 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6361 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6421 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6481 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6541 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6601 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6661 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6721 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6781 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     6841 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6901 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     6961 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     7021 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     7081 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     7141 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7201 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     7261 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     7321 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7381 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7441 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7501 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     7561 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     7621 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     7681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7801 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7861 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7921 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7981 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8041 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8101 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     8161 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     8221 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     8281 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8341 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8401 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8461 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8521 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8581 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8641 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8701 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8761 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8821 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8881 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8941 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9001 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9061 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9121 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9181 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     9241 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9301 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9361 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
     9421 tagtaccggg cccgctctag agatccgacg cgccatctct aggcccgcgc cggccccctc
     9481 gcacggactt gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat
     9541 ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat
     9601 actagctaca ttttacatga taggcttgga tttctataac ttcgtatagc atacattata
     9661 cgaagttata aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg
     9721 agactataag tatcccttgg agaaccacct tgttggctgc tgctaacgcg gtcccgttta
     9781 agagctaagc tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa
     9841 aaagtggcac cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt
     9901 ggataaccgt attaccg
//
