LOCUS       sequence-448705-        9729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector for delivery and expression of acGFP1-tagged GTSE1
            protein with S91, 262, 454, 724A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Garcia-Vazquez N, Gonzalez-Robles TJ, Lane E, Spasskaya D, Zhang Q, 
            Kerzhnerman M, Jeong YT, Collu M, Simoneschi D, Ruggles KV, Rona G, 
            Pagano M, Kaisari S
  TITLE     Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell 
            proliferation: relevance in cancer prognosis
  JOURNAL   bioRxiv 2024.06.26.600797
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.06.26.600797"
COMMENT     Sequence Label: pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A
FEATURES             Location/Qualifiers
     source          1..9729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2181..2190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4377..5096
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        5124..5623
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5159..5182)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5556..5575
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5644..6243
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5644..5663)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6140..6160
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6428..6860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6963..6985)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7121..7141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7126..7455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7306..7441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7477..7496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7577..7594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7748..8336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7828..7847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8507..9298,9299..9367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9130..9149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9368..9472)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9540..9558
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9658..9729,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgcc gccaccatgg atgaccctaa gaaggaagac attcttcttt
     2221 tggccgatga aaaatttgac ttcgatcttt cattgtcttc ttcgagtgca aatgaagatg
     2281 atgaagtctt cttcggaccc tttggacata aagaaagatg tattgctgcc agcttggaat
     2341 taaataatcc ggttcccgaa cagcctccgt tgcccacatc tgagagtccc tttgcctggg
     2401 cccctctggc cggggagaag ttcgtggagg tgtacaaaga agctcactta ctggctttac
     2461 acattgagag cagcagccgg aaccaggcag cccaagctgc caagcctgaa gaccctcgga
     2521 gccagggcgt ggaaagattc atacaggagt caaaattaaa aataaacctc tttgagaaag
     2581 aaaaggaaat gaagaaaagc cccacgtctc ttaaaaggga gacatactac ctgtcagaca
     2641 gccccttgct ggggccccct gtgggtgagc ctcggctctt ggcctcctcc ccggccctgc
     2701 ccagctctgg tgcccaggcc cgcctcaccc gggcgccggg gcctccgcac tctgctcatg
     2761 ctttgcccag ggaatcatgc actgctcatg ctgcaagtca ggcagcgact cagaggaagc
     2821 ccgggaccaa attgctgctg cctcgagcgg cctctgttag aggaagaagc atccctgggg
     2881 ctgcggagaa gcccaagaaa gagattccag ctgctccttc caggacaaaa atcccagctg
     2941 agaaggaatc ccaccgggat gttctccctg acaaacctgc cccgggtgct gtcaatgtgc
     3001 cggccgccgg aagccacttg ggccagggca agcgggcgat ccctgttcca aacaagttgg
     3061 ggctgaagaa gaccctgtta aaagcacccg gctctaccag caatctcgca aggaagtcct
     3121 cctcggggcc tgtttggagc ggggcatcca gtgcgtgcac atccccagca gtgggcaaag
     3181 ctaaatcaag tgaatttgca agtattcctg caaatagctc ccggcctctg tcaaacatca
     3241 gcaagtcagg cagaatggga cccgccatgc tgcggccagc tctgcctgca ggccctgtgg
     3301 gggcatcctc ctggcaggcc aagcgggtcg atgtttctga gctggcagcg gagcagctca
     3361 cggcaccccc ctcagcatcc cccacccaac cccagactcc ggaaggtggc ggccagtggc
     3421 tgaactccag ttgcgcttgg tcagaatctt ctcaattgaa taagactaga agtatcagac
     3481 ggcgagatgc ctgtctaaat tccaagacaa aggttatgcc tactcctaca aatcaattta
     3541 aaattcctaa gttttctatt ggtgactccc cggacagctc aacaccaaag ctttcgcggg
     3601 cacagcggcc gcagtcgtgc acgtcagttg gcagggtcac tgtccacagc accccggtta
     3661 gacgctcatc tgggccagca ccacaaagcc tgctgagcgc atggcgtgtg tcagccttgc
     3721 ccacacccgc cagccggcgc tgctctggcc ttccaccgat gacccccaaa acgatgccca
     3781 gggccgtggg ctctcccctg tgtgtgccag ctcggagacg ttcctctgag ccccgcaaga
     3841 actctgcaat gagaactgaa ccaacaaggg agagcaacag aaagacagat tccaggctgg
     3901 tggatgtgtc ccctgacagg ggttctcctc cttcccgtgt gcctcaggca cttaactttt
     3961 ctccagagga aagcgattct actttctcca aaagtactgc cacagaagta gctcgggagg
     4021 aagccaagcc gggtggagat gcagccccta gtgaggctct tcttgtagat atcaaactgg
     4081 aaccactcgc ggtcactcca gatgctgcaa gccagcccct cattgacctt cctctcatcg
     4141 acttctgcga taccccagaa gcacacgtgg ctgtaggatc tgaaagcagg cctctgatcg
     4201 acctcatgac aaacactcca gacatgaata aaaatgtggc caaaccttca ccggtggtgg
     4261 gacagctcat agacctgagc tcccctctga tccagctggc ccctgaggct gacaaggaga
     4321 acgtggattc cccactcctc aagttcggag gcggaggaag cggggatcca ccggtcatgg
     4381 tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg
     4441 atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca
     4501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg
     4561 tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc
     4621 acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg
     4681 aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat accctggtga
     4741 atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg ggcaataaga
     4801 tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc aagaatggca
     4861 tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc
     4921 actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc
     4981 tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctact
     5041 tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtgagcgg
     5101 ccgcgactct agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     5161 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     5221 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     5281 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     5341 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     5401 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     5461 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     5521 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     5581 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt
     5641 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5701 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5761 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5821 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5881 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5941 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6001 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6061 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6121 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6181 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6241 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     6301 gcgtctcgat cgagatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc
     6361 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
     6421 aggggggaat gaaagacccc acctgtaggt ttggcaagct actagcttaa gtaacgccat
     6481 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa
     6541 cagatggaac agggtcgatc gaccctagag aaccatcaga tgtttccagg gtgccccaag
     6601 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     6661 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     6721 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     6781 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     6841 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     6901 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     6961 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7021 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7081 acccagtcac gtagcgatag cggagtgtag atccggctgt ggaatgtgtg tcagttaggg
     7141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7441 ctaggctttt gcaaaaagct tactggctta actatgcggc atcagagcag attgtactga
     7501 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8821 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9121 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9481 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9541 ataggcgtat cacgaggccc tttcgtcttc aagaattagc ttggccattg catacgttgt
     9601 atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac
     9661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9721 atatggagt
//
