LOCUS       sequence-448723-       11591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium-mediated transformation. Plastid(thylakoid 
            membrane)-localized mCherry-UnaG. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Ishikawa K, Kodama Y
  TITLE     Bilirubin Distribution in Plants at the Subcellular and Tissue 
            Levels.
  JOURNAL   Plant Cell Physiol. 2024 Feb 27:pcae017. doi: 10.1093/pcp/pcae017.
  PUBMED    38466577
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2024 Feb 27:pcae017. doi: 10.1093/pcp/pcae017."
COMMENT     Sequence Label: pGWB602-tkUnaG
FEATURES             Location/Qualifiers
     source          1..11591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..41
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(52..471)
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
                     KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
                     GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     CDS             complement(502..1209)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(777..796)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2478..2502)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2524..2869)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2561..2581)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(3384..3401)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3384..3400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3393..3415)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3603..3627
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4928..5557
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5986..7059
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7125..7319
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7555..7577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7663..7803
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7718..7737
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7818..7835)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7989..8577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8069..8088)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8823..9614)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    10135..10159
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        10665..10844
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             10896..11447
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
ORIGIN
        1 ctaagcgctg ttatcaacca ctttgtacaa gaaagctggg tctagatatc ctcattccgt
       61 cgccctccgg tagctgcgca cagccacgac gtctcccatc gtaagtgtca cgaccagttt
      121 accgtccttt atctctcgga catacgtcgt ctccttgccg tcccactttt gtacgtacac
      181 cagcttctct cccaccaagt tcacgacaga ttttacgcct tttcttcgat cagaaggaaa
      241 ttcgtcgaac tcctccccta atttgaactt tacttgagtg tcaaggaacg taggaggtcc
      301 attctctatt ttcactgtca ttttgtctcc gtccttctgg gagatgtaca atgtcggcgt
      361 cgtggcatcc ccaccatcgc ttaattcctt tggggctccg atagctttca ggtattcacc
      421 aaaattatgg ctgtctgcga tcttccaggt gccaacaaat ttctcgacca taccagaacc
      481 accggatcca ccagaaccac ccttgtacag ctcgtccatg ccgccggtgg agtggcggcc
      541 ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa
      601 cttgatgttg acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt
      661 cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc
      721 cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt
      781 cttctgcatt acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat
      841 gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa
      901 gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat
      961 gtcggcgggg tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc
     1021 ccaggcgaag ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc
     1081 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc
     1141 catgtgcacc ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt
     1201 gctcaccata ccgccgctac cgccagattt atgccaacca tttgatgagt gcttaatttc
     1261 aaggatcctt ttctttgcga tgattcgatt gcgtatgacc tctttggatt caacagcttc
     1321 atcactgtta ctaagcacca tagcacatag ctcatctaaa gctagcattg aaaatggctc
     1381 ctctataaga acacccactt cttcaatgca gaagagagag gcagcactga tgaaagtagc
     1441 agggactaca ttccagtggc aatcatccca gagaataaca gggagtgtta gatgccataa
     1501 gactaagaac ctcgacgtta gtcgtgtgta ggaaagtgga atcggaattc ccattagttg
     1561 ttcacataca ccaattcctt cctgtaattg catcatcttt gtttccaaca tatctatctt
     1621 tgtactatcc aaattcagca gctgaaggct ctgagaaatg aattgaatga cacaacgtgg
     1681 acgatgcttg gattgaagaa tcaaagacag atcatctact tctatcacat tcttgagatc
     1741 actagcaata tctgaaccat aaatcacatg gcacttaagc gccacaggga aagcaacaat
     1801 gtaacgcaaa agcgcatctc tgatgatcaa ttcatcaccg gagccatgaa cagaagaaat
     1861 aactagccta gctaaatcat tggttccact aatgatctta acccaagctt tccttccttg
     1921 ttcaaacctt gaataagaag cctcagtgcg aaacactaga agcagagcta aagccggagc
     1981 cgtaagctga tacggtagag gagaagctcg aagtacaggg aagaaatcag gcaaccagtc
     2041 caaatccacg gcggagttat agccggcgat caggatcgcg acggtggtga agaagaaaac
     2101 aggcggaatc aaagacagta taacgcgtga agatgggctc gaggatacgt gacggagatg
     2161 gcgaagagag cttctgtgtc tgacccaatt ctcgtgagtg tagagagatc gtttctgctg
     2221 catccgtctc tctttgatac cgtcagacca gtttgggaca gctttaagca gcgaaatgag
     2281 cttctcggat agaggtgaat cgtcggattt ggggccggag gagacgcagg aaggattgaa
     2341 tttgaaatgt agggatttgg gagctctggc ggagattccg gagtataatc gtggtttgag
     2401 aaaggatagg ttggtaaagt tagaagaaaa agataggttc attgactgat acatgtcgac
     2461 tgaattggtt cctttaaagc ctgctttttt gtacaaactt gttgataact ctagagtccc
     2521 ccgtgttctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     2581 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     2641 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     2701 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca
     2761 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca
     2821 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg
     2881 gtcttctgag actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccaccat
     2941 gttgacgaag attttcttct tgtcattgag tcgtaagaga ctctgtatga actgttcgcc
     3001 agtctttacg gcgagttctg ttaggtcctc tatttgaatc tttgactcca tggcctttga
     3061 ttcagtggga actacctttt tagagactcc aatctctatt acttgccttg gtttgtgaag
     3121 caagccttga atcgtccata ctggaatagt acttctgatc ttgagaaata tatctttctc
     3181 tgtgttcttg atgcagttag tcctgaatct tttgactgca tctttaacct tcttgggaag
     3241 gtatttgatt tcctggagat tattgctcgg gtagatcgtc ttgatgagac ctgctgcgta
     3301 agcctctcta accatctgtg ggttagcatt ctttctgaaa ttgaaaaggc taatctgggg
     3361 acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3421 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3481 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc
     3541 tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
     3601 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     3661 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     3721 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
     3781 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     3841 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     3901 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     3961 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     4021 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     4081 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     4141 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     4201 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     4261 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
     4321 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
     4381 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
     4441 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
     4501 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
     4561 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
     4621 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
     4681 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc
     4741 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
     4801 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
     4861 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
     4921 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
     4981 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
     5041 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
     5101 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
     5161 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
     5221 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
     5281 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
     5341 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
     5401 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
     5461 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
     5521 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
     5581 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
     5641 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
     5701 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
     5761 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
     5821 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
     5881 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgtctg
     5941 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
     6001 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
     6061 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
     6121 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
     6181 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
     6241 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
     6301 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
     6361 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
     6421 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
     6481 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
     6541 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
     6601 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
     6661 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
     6721 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
     6781 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
     6841 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
     6901 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
     6961 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
     7021 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
     7081 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
     7141 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
     7201 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
     7261 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
     7321 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
     7381 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
     7441 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
     7501 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     7561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     7621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     7681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     7741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     7801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     8461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8641 attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa
     8701 ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc
     8761 taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga
     8821 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     8881 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     8941 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     9001 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     9061 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     9121 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     9181 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     9241 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     9301 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     9361 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     9421 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     9481 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     9541 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     9601 accgcttccc ccatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     9661 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     9721 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     9781 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     9841 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc ccgaattgat
     9901 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
     9961 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    10021 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    10081 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    10141 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
    10201 tttaatgtac tgaattaacg ccgaattagg ctgaatttat ttctattaat ctcgacggat
    10261 cagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt
    10321 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
    10381 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
    10441 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
    10501 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
    10561 tctgatggcg caggggatca agatcatgag cggagaatta agggagtcac gttatgaccc
    10621 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa
    10681 ggagccactc agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca
    10741 ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa
    10801 aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct catattcact
    10861 ctcaatccag atctgatccc ggggggcaat aagatatgag cccagaacga cgcccggccg
    10921 acatccgccg tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca
    10981 tcgagacaag cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg
    11041 acctcgtccg tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg
    11101 ccggcatcgc ctacgcgggc ccctggaagg cacgcaacgc ctacgactgg acggccgagt
    11161 cgaccgtgta cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc
    11221 acctgctgaa gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc
    11281 ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc
    11341 gggcggccgg cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca
    11401 gcctgccggt accgccccgt ccggtcctgc ccgtcaccga gatctgaacg gagtgcgcgt
    11461 ggcatcgccc gagttggagc tggtacggga actcatcgaa ctcaactggc atacccgcaa
    11521 tggtgaggtg gaaccgcggc ggatcgcgta cgaccgtgcc caggaggggg taccgagctg
    11581 gggacgtccc c
//
