LOCUS       sequence-448728-       11220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium-mediated transformation. Plastid(stroma)-localized
            mCherry-UnaG..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Ishikawa K, Kodama Y
  TITLE     Bilirubin Distribution in Plants at the Subcellular and Tissue 
            Levels.
  JOURNAL   Plant Cell Physiol. 2024 Feb 27:pcae017. doi: 10.1093/pcp/pcae017.
  PUBMED    38466577
REFERENCE   2  (bases 1 to 11220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2024 Feb 27:pcae017. doi: 10.1093/pcp/pcae017."
COMMENT     Sequence Label: pGWB602-ptUnaG
FEATURES             Location/Qualifiers
     source          1..11220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(161..178)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(161..177)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(170..192)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    380..404
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1705..2334
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2763..3836
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3902..4096
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4332..4354)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4440..4580
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4495..4514
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4595..4612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4766..5354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4846..4865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5600..6391)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6912..6936
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        7442..7621
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7673..8224
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      8384..8636
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8672..8693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8708..8713,8714..8731,8732..8738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8746..8762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8751..8773
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8770..8786
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8770..8786
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(8819..9071)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9103..9127
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9138..9557)
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
                     KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
                     GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     CDS             complement(9588..10295)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9863..9882)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(10556..10580)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(10675..11020)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10712..10732)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
       61 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
      121 ccatgttggc aagctgctct agccaatacg caagcttggc actggccgtc gttttacaac
      181 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      241 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      301 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc
      361 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
      421 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
      481 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
      541 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
      601 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
      661 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
      721 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
      781 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
      841 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
      901 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
      961 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     1021 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     1081 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     1141 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     1201 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     1261 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     1321 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     1381 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     1441 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     1501 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     1561 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     1621 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     1681 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     1741 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     1801 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     1861 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     1921 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     1981 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     2041 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     2101 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     2161 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     2221 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     2281 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     2341 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     2401 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     2461 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     2521 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     2581 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     2641 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     2701 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     2761 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     2821 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     2881 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     2941 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     3001 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     3061 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     3121 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     3181 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     3241 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     3301 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     3361 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     3421 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     3481 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     3541 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     3601 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     3661 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     3721 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     3781 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     3841 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     3901 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     3961 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     4021 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     4081 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     4141 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     4201 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     4261 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     4321 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4381 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4441 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     4501 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     4561 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4621 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4681 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4741 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4801 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4861 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4921 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4981 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5041 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5101 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5161 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5221 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5281 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5341 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5401 aaaactcacg ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt
     5461 attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat
     5521 tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga
     5581 acgaatttct agctagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
     5641 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
     5701 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
     5761 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
     5821 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
     5881 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
     5941 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
     6001 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
     6061 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
     6121 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
     6181 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
     6241 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
     6301 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     6361 tcgatacttc ggcgatcacc gcttccccca tgatgtttaa ctttgtttta gggcgactgc
     6421 cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     6481 cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaacagtca taacaagcca
     6541 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     6601 gtgagcgcat acgctacttg cattacagct tacgaaccga acaggcttat gtccactggg
     6661 ttcgtgcccg aattgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     6721 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     6781 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     6841 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     6901 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
     6961 aacacattgc ggacgttttt aatgtactga attaacgccg aattaggctg aatttatttc
     7021 tattaatctc gacggatcag cttcacgctg ccgcaagcac tcagggcgca agggctgcta
     7081 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
     7141 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
     7201 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
     7261 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
     7321 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tcatgagcgg agaattaagg
     7381 gagtcacgtt atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac
     7441 agaaccgcaa cgttgaagga gccactcagc cgcgggtttc tggagtttaa tgagctaagc
     7501 acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc
     7561 aaatatttct tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt
     7621 agagtctcat attcactctc aatccagatc tgatcccggg gggcaataag atatgagccc
     7681 agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac
     7741 catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc
     7801 gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga
     7861 ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta
     7921 cgactggacg gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg
     7981 ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt
     8041 cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc
     8101 cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt
     8161 ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat
     8221 ctgaacggag tgcgcgtggc atcgcccgag ttggagctgg tacgggaact catcgaactc
     8281 aactggcata cccgcaatgg tgaggtggaa ccgcggcgga tcgcgtacga ccgtgcccag
     8341 gagggggtac cgagctgggg acgtccccag ctcgaatttc cccgatcgtt caaacatttg
     8401 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     8461 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     8521 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     8581 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     8641 attaatagaa ataggcgcgc ctaattcagc ctaatgtgag ttagctcact cattaggcac
     8701 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8761 aatttcacac aggaaacagc tatgacatga ttacgaattg gttccggaac caattcccga
     8821 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     8881 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     8941 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     9001 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     9061 tttgaacgat cggggaaatt cgagctctaa gcgctgttat caaccacttt gtacaagaaa
     9121 gctgggtcta gatatcctca ttccgtcgcc ctccggtagc tgcgcacagc cacgacgtct
     9181 cccatcgtaa gtgtcacgac cagtttaccg tcctttatct ctcggacata cgtcgtctcc
     9241 ttgccgtccc acttttgtac gtacaccagc ttctctccca ccaagttcac gacagatttt
     9301 acgccttttc ttcgatcaga aggaaattcg tcgaactcct cccctaattt gaactttact
     9361 tgagtgtcaa ggaacgtagg aggtccattc tctattttca ctgtcatttt gtctccgtcc
     9421 ttctgggaga tgtacaatgt cggcgtcgtg gcatccccac catcgcttaa ttcctttggg
     9481 gctccgatag ctttcaggta ttcaccaaaa ttatggctgt ctgcgatctt ccaggtgcca
     9541 acaaatttct cgaccatacc agaaccaccg gatccaccag aaccaccctt gtacagctcg
     9601 tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag
     9661 tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc
     9721 acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc
     9781 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
     9841 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
     9901 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
     9961 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
    10021 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
    10081 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc
    10141 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
    10201 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
    10261 atggccatgt tatcctcctc gcccttgctc accatggaat cggtaaggtc aggaaggtaa
    10321 gagagagtct caaacttctt ctttccaatc ggaggccaca cctgcatgca gttaactctt
    10381 ccgccgttgc ttgtgatgga agtaatgtcg ttgttagcct tgcgggtggc tgggaaggca
    10441 gcggaggact taagtccgtt gaaaggagcg accatagtgg cctgagccgg agaggcaacc
    10501 atagtagcgg aagagagcat agaggaagcc atgtcgactg aattggttcc tttaaagcct
    10561 gcttttttgt acaaacttgt tgataactct agaaattgta aatagtaatt gtaatgttgt
    10621 ttgttgtttg ttgttgttgg taattgttgt aaaaatacac tggtgatttc agcgtgtcct
    10681 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
    10741 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
    10801 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    10861 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
    10921 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
    10981 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    11041 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
    11101 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
    11161 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
//
