LOCUS       sequence-448729-       11219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Agrobacterium-mediated transformation. Peroxisome-localized 
            mCherry-UnaG..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Ishikawa K, Kodama Y
  TITLE     Bilirubin Distribution in Plants at the Subcellular and Tissue 
            Levels.
  JOURNAL   Plant Cell Physiol. 2024 Feb 27:pcae017. doi: 10.1093/pcp/pcae017.
  PUBMED    38466577
REFERENCE   2  (bases 1 to 11219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2024 Feb 27:pcae017. doi: 10.1093/pcp/pcae017."
COMMENT     Sequence Label: pGWB602-poUnaG
FEATURES             Location/Qualifiers
     source          1..11219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             248..664
                     /codon_start=1
                     /product="bilirubin-binding green fluorescent protein from 
                     Anguilla japonica (Kumagai et al., 2013)"
                     /label=UnaG
                     /translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
                     KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
                     GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
     protein_bind    complement(687..711)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      743..995
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1028..1044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1028..1044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1041..1063)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1052..1068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1076..1082,1083..1100,1101..1106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1121..1142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(1178..1430)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1590..2141)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2193..2372)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2878..2902
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3423..4214
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4460..5048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4949..4968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5202..5219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5234..5374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5300..5319)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5460..5482
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5718..5912
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5978..7051)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7480..8109)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9410..9434
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     9622..9644
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9636..9653
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9637..9653
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gap             10139..10238
                     /estimated_length=100
     primer_bind     10402..10422
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        10411..10457
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    10554..10578
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     11015..11034
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
       61 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
      121 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
      181 ccgccactcc accggcggca tggacgagct gtacaagggt ggttctggtg gatccggtgg
      241 ttctggtatg gtcgagaaat ttgttggcac ctggaagatc gcagacagcc ataattttgg
      301 tgaatacctg aaagctatcg gagccccaaa ggaattaagc gatggtgggg atgccacgac
      361 gccgacattg tacatctccc agaaggacgg agacaaaatg acagtgaaaa tagagaatgg
      421 acctcctacg ttccttgaca ctcaagtaaa gttcaaatta ggggaggagt tcgacgaatt
      481 tccttctgat cgaagaaaag gcgtaaaatc tgtcgtgaac ttggtgggag agaagctggt
      541 gtacgtacaa aagtgggacg gcaaggagac gacgtatgtc cgagagataa aggacggtaa
      601 actggtcgtg acacttacga tgggagacgt cgtggctgtg cgcagctacc ggagggcgac
      661 ggaatccaag ttgtgaggat atctagaccc agctttcttg tacaaagtgg ttgataacag
      721 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
      781 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
      841 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
      901 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
      961 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg
     1021 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1081 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1141 taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat agatgacacc
     1201 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
     1261 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
     1321 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
     1381 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
     1441 gagctgggga cgtccccagc tcggtacccc ctcctgggca cggtcgtacg cgatccgccg
     1501 cggttccacc tcaccattgc gggtatgcca gttgagttcg atgagttccc gtaccagctc
     1561 caactcgggc gatgccacgc gcactccgtt cagatctcgg tgacgggcag gaccggacgg
     1621 ggcggtaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc
     1681 ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc
     1741 acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc
     1801 agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg
     1861 gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc
     1921 gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc
     1981 cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg
     2041 accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg
     2101 gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tatcttattg ccccccggga
     2161 tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga
     2221 acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact
     2281 tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc
     2341 gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt
     2401 cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat
     2461 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     2521 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     2581 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     2641 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     2701 tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg
     2761 cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta attcggcgtt
     2821 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     2881 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     2941 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt
     3001 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct
     3061 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag
     3121 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tgttcggttc
     3181 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     3241 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     3301 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     3361 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     3421 tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     3481 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     3541 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     3601 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     3661 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     3721 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     3781 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     3841 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     3901 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     3961 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     4021 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     4081 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     4141 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     4201 tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg
     4261 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca
     4321 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat
     4381 catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4441 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4501 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4561 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4621 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4681 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4741 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4801 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4861 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4921 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4981 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5041 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5101 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5161 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5221 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5281 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     5341 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     5401 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5461 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc
     5521 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     5581 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     5641 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     5701 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     5761 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     5821 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     5881 gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc
     5941 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     6001 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     6061 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     6121 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     6181 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     6241 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     6301 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     6361 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     6421 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     6481 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     6541 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     6601 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     6661 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     6721 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     6781 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     6841 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
     6901 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     6961 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     7021 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     7081 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     7141 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     7201 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     7261 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     7321 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     7381 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     7441 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     7501 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     7561 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     7621 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     7681 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     7741 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
     7801 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
     7861 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
     7921 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
     7981 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
     8041 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
     8101 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
     8161 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
     8221 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
     8281 aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc
     8341 gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg
     8401 tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct
     8461 cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct
     8521 cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct
     8581 ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg
     8641 tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc
     8701 gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca
     8761 ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg
     8821 cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc
     8881 ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca
     8941 gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg
     9001 ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat
     9061 agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc
     9121 aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg
     9181 cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca
     9241 ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat
     9301 cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc
     9361 acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc
     9421 aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca atctgatcca
     9481 agctcaagct gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     9541 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     9601 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     9661 ttgcgtattg gctagagcag cttgccaaca tggtggagca cgacactctc gtctactcca
     9721 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
     9781 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
     9841 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
     9901 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     9961 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg
    10021 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg
    10081 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ggaaaggcta tcgttcaaga
    10261 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
    10321 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
    10381 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
    10441 atttcatttg gagaggacac gctgaaatca ccagtgtatt tttacaacaa ttaccaacaa
    10501 caacaaacaa caaacaacat tacaattact atttacaatt tctagagtta tcaacaagtt
    10561 tgtacaaaaa agcaggcttt aaaggaacca attcagtcga catggtgagc aagggcgagg
    10621 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
    10681 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
    10741 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
    10801 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
    10861 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
    10921 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
    10981 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
    11041 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
    11101 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
    11161 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatc
//
