LOCUS       sequence-44877-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Bhakta MS, Segal DJ
  TITLE     The generation of zinc finger proteins by modular assembly.
  JOURNAL   Methods Mol Biol. 2010;649:3-30.
  PUBMED    20680825
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2010"; volume: "649"; pages: "3-30"
COMMENT     Sequence Label: pSSA-1-3
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(137..155)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggcattccg gtactgttgg
       61 taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct
      121 ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      181 tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt
      241 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      301 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      361 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      421 tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt
      481 gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga
      541 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
      601 tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa
      661 ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt
      721 gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
      781 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
      841 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct
      901 tcaggattac aagattcaaa gtgcgctgct
//
