LOCUS       sequence-448814-        2552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates GNB1L dTAG C terminal  knock-in cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, 
            Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J
  TITLE     FACS-based genome-wide CRISPR screens define key regulators of DNA 
            damage signaling pathways.
  JOURNAL   Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 
            10.1016/j.molcel.2023.07.004.
  PUBMED    37541219
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.07"; journalName: "Mol Cell"; date: 
            "2023-08-3- 3"; volume: "83"; issue: "15"; pages: "2810-2828.e6"
COMMENT     Sequence Label: pUC19_GNB1L_dTAG
FEATURES             Location/Qualifiers
     source          1..2552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             986..1306
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1316..1342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1316..1342
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1346..1372
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1376..1432
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1433..1831
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 acgtaggacc tgtctgtgtg gctccgggtc tcgtgcagtg ccctaccccc tctcagaacc
       61 cagccgggga cgttgccccc accaggcaag gtacactgtg tggctcctgc cgcctccagg
      121 gcgtctcccc cttgtccccc cctcatcctg ccaacccccc actgggtctt gctcttggca
      181 gcgctcccag ccctgtcgag cttgggggtt cacatacctc atggttttgc tgaaaatgga
      241 gtttgtattt ctcaccctcg tcagctttgg ggtggttgtg aggttcttct gggaggagtg
      301 ccagcattct gctgccatca agcccgggac gtggcctctc gctaggcact agggtgcagt
      361 ggcttccctg gggtgagggg tcccgggacc cagcccctct cctgcagctg ggacctccct
      421 gtctctgctg ctctctgact ggggaaggag tcggtcagag ctggtgcgag gagcagcagg
      481 gcagggccga agaccactct gaggggaggg ctggagtccc ccatcgtggc cttaggaaga
      541 gtgggaggtc agctgggtag gagcgtgtgg ccctcacatc ccgggcctgt gggcctgcgt
      601 ctctcctggt tccagctgca ggtggggcac caggggcagc actgccttgt gggcttaagg
      661 agtggcctaa cacctctccc aactctcaca ggtgcgtggg actcatgaac tcaccaatcc
      721 cgggatcgcc gaggtcacga tccggccaga tcgcaagatc ctggccaccg caggctggga
      781 ccaccgcatc cgcgtgttcc actggcggac gatgcagcca ctggccgtgc tggccttcca
      841 cagcgccgct gtccagtgcg tggccttcac cgccgatggc ttgctggccg cgggctccaa
      901 ggatcagcgg atcagtttat ggtcactcta cccacgcgca ggtggcggtg gctcgggcgg
      961 tggtgggtcg ggtggcggcg gatctggagt gcaggtggaa accatctccc caggagacgg
     1021 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
     1081 tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
     1141 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
     1201 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
     1261 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaggct cgggctaccc
     1321 ctacgacgtg cccgactacg ccggctatcc gtatgatgtc ccggactatg caggcgctac
     1381 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctatggccaa
     1441 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     1501 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     1561 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     1621 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     1681 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     1741 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     1801 gctgccctct ggttatgtgt gggagggctg actcacccac tcccttccat agagacgagg
     1861 agggcgggca gggaggtggg catcaggccc cagccttggc ctgaaacctc aagggcctct
     1921 gaggaccagc aagtcatggc ccaaggacca tggagtcccc gctgtcttca tgaggttggt
     1981 atttcctttt gtggagtgcc tcatcacagg acggtgactc tgggggccag ccagagccct
     2041 ggccgtccga ggcctgcagg agacgttggc ttgggcctgc tgtgttgcca gctgagggta
     2101 ttttataata aatttccatt gccaagttgc aggcttgagg tgggagctcc ctgaggaggc
     2161 cctggtcccc cacttagcat cagtgccctc agacccccac caggcaggat gctcagcccc
     2221 accctcacac ctgctgttga atgggccacc ccgagtgtgg gtttcctggg ccaggcctca
     2281 ggagatgata ggccccaacc atagctgcgt cgatgtccac atgacccctc accccgagga
     2341 ggccaggagc tcccaacaca caggttgagg ccaggatcag gatcaggaag taactgaact
     2401 ccctaaagag tgaagcagag aaaggtgcct gtgagggacg gcaagggggg cttgcagcgc
     2461 acagggccag gccagatgtg ggatccggga gggcacgcct gcccgcagag ggtgcagtgg
     2521 ggaaggcgca cagggtgcac ctccaggtga gt
//
