LOCUS       sequence-448815-        2206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates TELO2 dTAG N terminal knock-in cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, 
            Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J
  TITLE     FACS-based genome-wide CRISPR screens define key regulators of DNA 
            damage signaling pathways.
  JOURNAL   Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 
            10.1016/j.molcel.2023.07.004.
  PUBMED    37541219
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.07"; journalName: "Mol Cell"; date: 
            "2023-08-3- 3"; volume: "83"; issue: "15"; pages: "2810-2828.e6"
COMMENT     Sequence Label: pUC19_TELO2_dTAG
FEATURES             Location/Qualifiers
     source          1..2206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             495..890
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             900..956
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             957..983
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             957..983
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             987..1013
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1023..1343
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ccgtaagctg ttggtttcta aggggcggag gaatggcgac tggagccacc tctcaccgct
       61 cagggagccg ggagggccgg cagctcccgc gggccgagtt tcccgccttg ggtgcgagga
      121 cgcgtggggc cgcgctgtgc ccggggaaca ctcaccagca tctctagcct ccacctccct
      181 ctcgcgccag tggcactccc ggttgagaca actgagaatg ttccctgggc acagtggccc
      241 gggttgagaa gccctgcagt ggctggtagc gttggggtcc gagcggagga gcgaactctg
      301 ggtggaaacc ggcaggcact ggaggggaag gaggcgggac agggttgggt gggaccaggg
      361 ttgaggggtg tggggtctcg gggcgctcac cgaggggctt cctgagcttg tgtggattat
      421 ttccgtgccc caagctgagc cctgtgtcca tgtcacaggt cgtcttcccg tgacgcccag
      481 atctgtgcta ctggatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
      541 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
      601 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
      661 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
      721 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
      781 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
      841 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ggaagcggag
      901 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggaccttacc
      961 cctacgacgt gcccgactac gccggctatc cgtatgatgt cccggactat gcaggaagcg
     1021 gaggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc
     1081 agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt gattcctccc
     1141 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg
     1201 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt
     1261 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg
     1321 atgtggagct tctaaaactg gaaggtggcg gtggctcggg cggtggtggg tcgggtggcg
     1381 gcggatctat ggagccagca ccctcagagg ttcgactcgc cgtccgggaa gccattcatg
     1441 ccctctcgtc ttcggaggat ggcggccaca tcttctgcac cctggagtcc ctgaagcggt
     1501 atctcggtga gatggagcct ccagcgctcc cgagggagaa ggaggagttt gcctcggccc
     1561 acttctcgcc tgtcctcaga tgtcttgcca gcaggctgag cccagcctgg ctggagctgc
     1621 tgccccatgg ccgcctggag gagctgtggg ccagcttctt cctggagggc ccggcggacc
     1681 aagccttcct ggtgttgatg gagaccatcg agggtgctgc ggggtgagtg ggctgggccc
     1741 atcctggggt tgccggtagc ctcagaagtg atgagagtgg cttgaaggac tggaccaaga
     1801 gcctctctag tccctgtgag gggctagaga gagagcctgc tcctggctga acccctgaac
     1861 agaagaagcg gtctgtgtct gtctcctttg cgacgggagg cacctgctgt gtctcacaaa
     1921 gtcccccact tgctccccgt ccggctgtgt cagagaggga tggggtggga gtgttcacat
     1981 cccaggcggc agaggcagcc cgtcagctgg ggacgttcca ggttttccag ggaagcacag
     2041 ctgtcatcac tgacacgtgt cccatgtgga ctcccagccc cagccggctc ccaggctcag
     2101 cctcagatca cactgcagtc cttgccggga cactgcatct tgagaagctg ggttttcaga
     2161 ggttgctgtg atcaaagcaa gcactgagtt atccgcgtca gcagca
//
