LOCUS       sequence-44883-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bhakta MS, Segal DJ
  TITLE     The generation of zinc finger proteins by modular assembly.
  JOURNAL   Methods Mol Biol. 2010;649:3-30.
  PUBMED    20680825
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2010"; volume: "649"; pages: "3-30"
COMMENT     Sequence Label: pPGK-GZF1-RR
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..71
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             99..119
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccactcccag gtccaactgc acctcggttc tatcgattga catgtaccca tacgatgtcc
       61 cagactacgc gaattccccg gggatcccag gcatggggcc caaaaagaaa cgcaaagttg
      121 ggcgcctcga gcccggggag aagccctatg cttgtccgga atgtggtaag tccttcagca
      181 ccagtggcca cttggttaga caccagcgta cccacacggg tgaaaaaccg tataaatgcc
      241 cagagtgcgg caaatctttt agcaccagcg gcaacctggt gcgccatcaa cgcactcata
      301 ctggcgagaa gccatacaaa tgtccagaat gtggcaagtc tttcagccag tccagcaacc
      361 tggtgcgcca ccaacgtact cacaccggtg cggccgcccg cgccctagtc aaaagtgaac
      421 tggaggagaa gaaatctgaa cttcgtcata aattgaaata tgtgcctcat gaatatattg
      481 aattaattga aattgccaga aattccactc aggatagaat tcttgaaatg aaggtaatgg
      541 aattttttat gaaagtttat ggatatagag gtaaacattt gggtggatca aggaaaccgg
      601 acggagcaat ttatactgtc ggatctccta ttgattacgg tgtgatcgtg gatactaaag
      661 cttatagcgg aggttataat ctgccaattg gccaagcacg agaaatgcaa cgatatgtcg
      721 aagaaaatca aacacgaaac aaacatatca accctaatga atggtggaaa gtctatccat
      781 cttctgtaac ggaatttaag tttttatttg tgagtggtca ctttaaagga aactacaaag
      841 ctcagcttac acgattaaat catatcacta attgtaatga
//
