LOCUS       sequence-44898-e       13277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13277)
  AUTHORS   Zhang F, Maeder ML, Unger-Wallace E, Hoshaw JP, Reyon D, Christian 
            M, Li X, Pierick CJ, Dobbs D, Peterson T, Joung JK, Voytas DF
  TITLE     High frequency targeted mutagenesis in Arabidopsis thaliana using 
            zinc finger nucleases.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Jun 29. 107(26):12028-33.
  PUBMED    20508152
REFERENCE   2  (bases 1 to 13277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Jun 29. 107(26):12028-33."
COMMENT     Sequence Label: pFZ19
FEATURES             Location/Qualifiers
     source          1..13277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        197..243
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             272..523
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     CDS             539..772
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             782..1717
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             830..1723
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        2281..2460
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2313..2496
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      3611..3863
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4184..4230
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    4279..4403
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4428..4433,4434..4451,4452..4458)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4512..5192
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(4578..4597)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5512..5817
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5696..5715
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5858..5982)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    6499..6673
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6751..6775
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7200..7994
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8081..8669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8570..8589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8823..8840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8855..8995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8921..8940)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9081..9103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9339..9533
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9599..10672)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11101..11730)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13031..13055
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13243..13265
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13257..13274
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13258..13274
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcccaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag
       61 ctaagcttgc atgcctgcag gatatcgtgg atccaagctt gccacgtgcc gccacgtgcc
      121 gccacgtgcc gccacgtgcc tctagaggat ccatctccac tgacgtaagg gatgacgcac
      181 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      241 ggacacgctg ggatccccaa ttccgggcgg aatgaaagcg ttaacggcca ggcaacaaga
      301 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
      361 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
      421 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
      481 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgtcgagcgc
      541 ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat
      601 ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc
      661 gggtccggga tttacccccc acgactccgc cccctacggc gctctggata tggccgactt
      721 cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg gggatccgtc
      781 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
      841 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
      901 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
      961 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
     1021 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
     1081 ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagggaagct
     1141 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
     1201 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
     1261 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
     1321 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
     1381 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
     1441 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
     1501 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
     1561 gatgctggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
     1621 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
     1681 ttacatcacg ggggaggcag agggtttccc tgccacagtc tgagagctcc ctggcggaat
     1741 tcccagagat gttagctgaa atcatcacta atcagatacc aaaatattca aatggaaata
     1801 tcaaaaagct tctgtttcat caaaaatgac tcgacctaac tgagtaagct agcttgttcg
     1861 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact
     1921 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta
     1981 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt
     2041 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat
     2101 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca ctagatcccc
     2161 aaacaagctt gaaactgaag gcgggaaacg acaatctgat catgagcgga gaattaaggg
     2221 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca
     2281 gaaccgcaac gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca
     2341 catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca
     2401 aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta
     2461 gagtctcata ttcactctca atccaaataa tctgcaccgg atccgctaga ggatctcgac
     2521 ctgcaagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc
     2581 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     2641 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     2701 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     2761 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     2821 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     2881 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     2941 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     3001 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     3061 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     3121 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     3181 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     3241 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     3301 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     3361 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     3421 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     3481 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     3541 cgccccagca ctcgtccggg atcttggagg tgatgtaaca tgatcacaag ctgatccccc
     3601 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     3661 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     3721 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     3781 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     3841 ggtgtcatct atgttactag atcgggaatt gatcccccct cgacagcttg catgccgctt
     3901 gggctgcagg tcgaggctaa aaaactaatc gcattatcat cccctcgacg tactgtacat
     3961 ataaccactg gttttatata cagcagtact gtacatataa ccactggttt tatatacagc
     4021 agtcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4081 actggtttta tatacagcag tcgaggtaag attagatatg gatatgtata tggatatgta
     4141 tatggtggta atgccatgta atatgctcga ctctaggatc ttcgcaagac ccttcctcta
     4201 tataaggaag ttcatttcat ttggagagga cacgctgaag ctagtcgact ctagcctcga
     4261 ggcgcgccaa gctatcaaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
     4321 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     4381 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
     4441 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     4501 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     4561 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     4621 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     4681 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     4741 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     4801 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     4861 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     4921 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     4981 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     5041 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     5101 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     5161 cggggcgtaa tctagaggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     5221 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     5281 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     5341 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     5401 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     5461 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     5521 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     5581 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     5641 gataaagtcc cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     5701 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     5761 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     5821 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     5881 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     5941 atcattttac gtttctcgtt cagctttctt gtacaaagtg gttcgataat tcttaattaa
     6001 ctagtcgatc caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa
     6061 gttcaatgca tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg
     6121 cattggaaaa gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca
     6181 gtttttgttt tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt
     6241 ccttttgttc attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta
     6301 agtaagaaag gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct
     6361 ttcacctctc tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa
     6421 gtttactatg agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttt
     6481 ttgtgtcgag ggggtacctt tctccataat aatgtgtgag tagttcccag ataagggaat
     6541 tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     6601 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac
     6661 taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa
     6721 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     6781 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     6841 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     6901 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     6961 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt
     7021 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     7081 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     7141 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     7201 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     7261 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     7321 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     7381 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     7441 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     7501 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     7561 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     7621 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     7681 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     7741 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     7801 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     7861 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     7921 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     7981 atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8221 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8821 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     8881 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     8941 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     9001 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     9061 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     9121 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
     9181 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
     9241 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
     9301 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
     9361 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
     9421 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
     9481 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
     9541 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
     9601 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
     9661 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
     9721 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
     9781 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
     9841 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
     9901 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
     9961 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
    10021 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
    10081 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
    10141 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
    10201 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
    10261 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
    10321 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
    10381 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
    10441 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
    10501 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
    10561 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
    10621 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
    10681 ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc
    10741 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
    10801 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
    10861 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
    10921 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
    10981 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
    11041 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
    11101 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
    11161 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
    11221 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
    11281 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
    11341 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
    11401 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
    11461 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
    11521 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
    11581 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
    11641 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
    11701 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
    11761 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
    11821 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
    11881 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
    11941 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
    12001 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
    12061 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
    12121 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
    12181 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
    12241 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
    12301 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
    12361 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
    12421 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
    12481 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
    12541 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
    12601 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
    12661 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
    12721 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
    12781 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
    12841 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
    12901 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
    12961 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
    13021 tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc
    13081 gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca
    13141 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    13201 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    13261 aaacgacggc cagtgcc
//
