LOCUS       sequence-44901-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mid2(SS/TM), containing only the signal sequence and 
            transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A 
            mutations)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS275
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(500..520)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtatccaccg ctttatcaat ctcttctgca ggttttcttc gccagcatga ctgcctctct
       61 ctcggcagca tctcggacat gctcagttcc atccaactga accccaataa agtactggac
      121 atctcccttc tgatctcgca taggctgcaa gtgaaagagg ttccagaact ttttaccact
      181 ctttgtataa ttaatcagct gaacagtgac ctctgtttgg ttatctatgg catctctaat
      241 ttttctcact gtcgcgcgat cagtttcagg accttgtaga aacctgcagt ttcttcccaa
      301 aatttcttca cggctatatt ctgtcaactg caagaaacta tcggacgcga atataatggg
      361 attatctggc aatcttgggt cagtaatgac aaagttcttc tcaatacgtt caagtgcagc
      421 agccaaccta ggacttccac ctgaacctcc agatccacct ttgtatagtt catccatgcc
      481 atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt
      541 gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta aaaggacagg
      601 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc ttccatcttc
      661 aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt ctgccatgat
      721 gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt ttccatcttc
      781 tttaaaatca
//
