LOCUS       sequence-44903-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mid2(SS/TM), containing only the signal sequence and 
            transmembrane helix, fused to GFP and LOVpep(V529N mutation)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS277
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(488..508)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttatcaatc tcttctgcag ttttcttaat cagcatgttt gcctctctct cggcagcatc
       61 tcggacatgc tcagttccat ccaactgaac cccaataaag tactggacat ctcccttctg
      121 atctcgcata ggctgcaagt gaaagaggtt ccagaacttt ttaccactct ttgtataatt
      181 aatcagctga acagtgacct ctgtttggtt atctatggca tctctaattt ttctcactgt
      241 cgcgcgatca gtttcaggac cttgtagaaa cctgcagttt cttcccaaaa tttcttcacg
      301 gctatattct gtcaactgca agaaactatc ggacgcgaat ataatgggat tatctggcaa
      361 tcttgggtca gtaatgacaa agttcttctc aatacgttca agtgtagtag ccaacctagg
      421 acttccacct gaacctccag atccaccttt gtatagttca tccatgccat gtgtaatccc
      481 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      541 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
      601 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
      661 aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
      721 tgagttatag ttgtattcca atttgtgtcc
//
