LOCUS       sequence-449052-       11672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor ZNF831 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11672)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-ZNF831-Brd 378
FEATURES             Location/Qualifiers
     source          1..11672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(122..139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(122..138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      280..735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     577..598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        761..865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(866..934,935..1726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1084..1103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1897..2485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2386..2405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2639..2656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2809..2814,2815..2832,2833..2839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2852..2874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2871..2887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2906..2926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4026..4259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4444..4488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4906..5312
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    10622..11025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             11144..11377
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11449..11570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11486..11505)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11540..11559
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(11610..11672,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagctgg ctgcagtaac gccattttgc
     4921 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     4981 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     5041 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     5101 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     5161 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     5221 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     5281 gcgccagtcc tccgacagac tgagtcggcc ggtcgaatca agcttatcga taccgtcgac
     5341 ggatccgcta gcccgcggtt cgaaatggag gttccagaac ccacctgccc tgcccctcct
     5401 gcgagggacc agccagctcc cactcctggc cctccagggg ccccaggtgg ccaggcctca
     5461 cctcacctga ccctgggccc tgtccttctg ccgccagagc agggcctggc cccccccact
     5521 gtgttcctga aggccctgcc catcccactg taccacacgg tgcctcccgg gggcctccag
     5581 ccccgcgccc cgctagtgac gggcagccta gatgggggca acgtgccctt catactcagc
     5641 cctgtgctgc agcctgaagg gcctggcccc acccaggtgg ggaagccggc ggcccctacg
     5701 ctgacggtga acatcgtggg cactctgcct gtcctgtcgc cgggcctggg ccccacgctg
     5761 ggcagcccag gcaaggtgcg gaatgcgggc aagtacctgt gtccgcactg tggtcgcgac
     5821 tgcctgaagc ccagtgttct agagaagcac atccggtccc acacgggtga gaggcccttc
     5881 ccgtgtgcca cctgcggcat cgcctttaag acccagagca atctctacaa gcacaggcgg
     5941 acgcagacgc acctcaacaa ctcccggctg tcctcagagt ccgagggcgc cgggggcggc
     6001 ctcctggagg aaggggacaa ggccggagag ccccccagac cagagggcag gggcgagagc
     6061 aggtgccagg ggatgcacga aggcgcctcg gagagacccc tttctccggg tgcccacgtg
     6121 cccctacttg ccaagaacct ggatgtgagg accgaagctg ctccctgtcc agggtccgca
     6181 tttgccgaca gagaggctcc ttgggactct gcccccatgg cgtcacctgg gctcccagcg
     6241 gccagcacac aaccctggcg taagttgcca gagcagaagt cgccgaccgc cgggaagccg
     6301 tgcgccctgc agcggcagca ggcgacggca gcggagaagc cctgggatgc caaggccccc
     6361 gagggccggc tgcggaagtg tgagagcacc gactcggggt acctgtcgcg ctccgacagc
     6421 gcggagcagc cgcatgcgcc ctgcagcccc ctgcacagcc tttcggagca cagcgccgag
     6481 tccgaggggg agggcggccc gggcccgggg ccaggggtcg caggggccga gcccggggcg
     6541 cgagaagccg gcctggagct ggagaagaag cggctggagg agcgcatcgc ccagctcatc
     6601 tcccacaacc aggcggtggt ggacgatgcc cagctggaca acgtgcggcc ccggaagacc
     6661 gggctgtcca aacagggcag catcgacctg cccacgccct acacctacaa ggactccttc
     6721 cactttgaca tccgcgcgct ggagccaggc cgtaggaggg ccccgggccc cgtgcgctcc
     6781 acctggacgc ccccagacaa gtctcggccc ctcttcttcc actccgtccc cactcagctc
     6841 tccaccaccg tggaatgtgt ccccgtcacc aggagcaact cgctgccctt cgtcgagggc
     6901 tccaggacgt ggctggagcc cagggagccc cgggacccct ggtccaggac gcagaagcct
     6961 ctgagcccca ggcccggccc agcccgcctg ggctgccgct cgggactaag ctcgactgac
     7021 gttcccagtg ggcatccccg ggccctggtc agacaggccg cggtggagga cctgccaggc
     7081 acccccattg gcgatgccct ggtgcccgca gaggacacag acgcaaagag aactgctgcg
     7141 cgggaggcca tggccggcaa gggcagagcg ggcggcagga agtgcggcca gagaaggctg
     7201 aagatgttct cccaggagaa gtggcaggtg tacggggatg agacgttcaa aaggatctac
     7261 cagaaaatga aagccagtcc ccatggaggc aagaaagcca gggaggtggg aatgggcagt
     7321 ggggcagaac tgggctttcc tctgcagaaa gaggcagcag ggagctcagg cacagtcccc
     7381 acccaagaca ggaggacccc tgtccatgag gacatatccg caggggcaac gccagagcct
     7441 tggggaaatc caccagccct ggaggcctcc ttggtgactg aacccactaa gcatggggag
     7501 acggtggcca ggagaggaga cagtgaccga cccagggtgg aagaggctgt gtcatcccct
     7561 gcactgggtg gcagagacag tccctgttca ggcagtagga gccccctggt ctctccaaat
     7621 gggaggctgg aactggggtg gcagatgccc ccagcacctg gccccctcaa agggggtgat
     7681 gtagaggctc ccaggccagt ttggccggac cccaagctgg aaggaggtgc ccgaggtgtg
     7741 ggggatgttc aggagacctg cctgtgggcc cagactgtcc tgagatggcc cagcaggggc
     7801 tcaggggagg acaagctccc ctcagagagg aagaagctga aagtggagga cctgcacagc
     7861 tggaagcaac cagagcctgt gagcgcagag accccaggtg ggcccacgca gcctgcctct
     7921 ttgtcatccc agaagcagga tgccgatccc ggggaggtgc cagggggctc aaaggagagt
     7981 gccaggcagg tgggcgagcc tctggagtcc tctggagcct ccttggctgc tgcttctgtt
     8041 gccctgaaga gggtggggcc aagggacaag gctaccccac tgcatcctgc agccccagcc
     8101 cccgcagagc acccctcgct ggccacccca cctcaggctc ctagagtgct ctctgccctg
     8161 gcagataatg ccttttcccc caagtacctc ctcaggttac ctcaggcaga gaccccctta
     8221 ccactgccca ttccctgggg accaaggcac agccaggact ctctctgcag cagtgggtgg
     8281 cctgaagaac gggcatcatt tgttgggtca ggactgggga cccctctttc tcccagccca
     8341 gcctcaggcc cctccccagg tgaggcggac agcatcctgg aggaccccag ctgttccagg
     8401 ccacaggatg ggagaaaagg ggcacagttg gggggggaca agggggacag gatggccact
     8461 agcaggccag cagccaggga gttgcccatc tcagcaccag gggctcccag ggaggctacc
     8521 tcctccccgc ccactccaac gtgtgaggca cacttagttc aggacatgga gggtgacagc
     8581 caccgtatcc atcgcctctg catgggcagc actttggcaa gggccaggct ctctggggat
     8641 gtcctgaatc cctgggtacc caactgggag ctgggggagc ctcctgggaa tgccccagaa
     8701 gatccttctt cagggcccct ggtgggcccc gacccgtgtt cccccctcca gcctggctcc
     8761 ttcctcactg ccctcactcg gcctcagggt gtgcccccag gctggccaga gctggccttg
     8821 tcttcccact cagggacgtc ccggagccac agcacccgca gtccccacag cacccaaaac
     8881 ccctttccct cactgaaggc tgagccgcgg ctcacgtggt gttgcctgag ccgcagtgtc
     8941 cctctgcccg cggagcagaa ggcaaaggcg gcatctgtgt acttggcggt gcactttcct
     9001 ggtagcagcc tccgagatga gggtcccaat ggccctcctg ggagcaatgg aggatggacc
     9061 tggacaagcc ctggagaagg agggccggcg cagatgtcca agttctccta cccaacagtc
     9121 ccaggggtga tgccccagca ccaggtgtct gagccagaat ggaagaaagg cctgccttgg
     9181 agggcaaaga tgtctcgtgg gaacagcaag cagagaaaac tgaagatcaa ccctaaaagg
     9241 tacaaaggga atttcttgca gagctgtgtt cagctgagag ccagtagact tcgcacacca
     9301 acctgggtgc gaagaagaag ccgccaccct cccgcacttg agggactgaa gccatgcagg
     9361 acccctgggc agacctcttc agaaatagca ggtctgaatc tgcaagagga gccatcttgt
     9421 gccacctcag aatcacctcc ttgttgtggg aaggaagaga agaaggaagg tgactgcaga
     9481 caaaccttag gaaccctctc tcttggtaca agttcaagaa ttgtcaggga aatggacaaa
     9541 cgaactgtga aggatatttc tccatctgct ggtgagcatg gtgactgtac tactcacagc
     9601 actgctgcca catcaggatt atctctgcaa tctgacacct gcctggcagt ggttaatgac
     9661 gtgcctctac cccctggcaa aggtcttgac cttgggttgc tggagactca gctgctggcc
     9721 tcccaggatt cagtctcaac agatcccaaa ccatacatct tctcagatgc tcaaaggcct
     9781 tcttcctttg ggtccaaagg aacttttccc caccatgaca ttgctacctc tgtggctgcc
     9841 gtttgtattt ctctgccagt gagaacagat cacatagccc aggaaattca cagtgctgaa
     9901 tcacgagacc acagccagac tgcagggagg actctgacat caagctcccc agacagcaaa
     9961 gtcacagaag agggcagagc acagaccctc ttgccaggga gaccttcatc tggacaaaga
    10021 atttcagatt cggttccact ggagtcaact gaaaaaactc atcttgaaat accagcttca
    10081 ggaccaagtt cagctagttc acaccacaag gaagggagac acaagacgtt ttttccttcc
    10141 agaggccagt atgggtgtgg ggaaatgact gtcccctgcc cctctttagg aagtgacggt
    10201 aggaaacgtc aggtatctgg attaatcact cggaaagatt ctgtggttcc ttctaagcca
    10261 gagcagccca tagaaattcc tgaagcccct tctaaatccc tcaagaagag gagtctggaa
    10321 ggaatgagaa agcaaactcg agtagagttc agtgacacca gcagcgacga tgaagaccga
    10381 ttagttatag aaatatagac cggtcggatc ccccgggctg caggaattcg agcatcttac
    10441 cgccatttat tcccatattt gttctgtttt tcttgatttg ggtatacatt taaatgttaa
    10501 taaaacaaaa tggtggggca atcatttaca tttttaggga tatgtaatta ctagttcagg
    10561 tgtattgcca caagacaaac atgttaagaa actttcccgt tatttacgct ctgttcctgt
    10621 taatcaacct ctggattaca aaatttgtga aagattgact gatattctta actatgttgc
    10681 tccttttacg ctgtgtggat atgctgcttt aatgcctctg tatcatgcta ttgcttcccg
    10741 tacggctttc gttttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    10801 gtggcccgtt gtccgtcaac gtggcgtggt gtgctctgtg tttgctgacg caacccccac
    10861 tggctggggc attgccacca cctgtcaact cctttctggg actttcgctt tccccctccc
    10921 gatcgccacg gcagaactca tcgccgcctg ccttgcccgc tgctggacag gggctaggtt
    10981 gctgggcact gataattccg tggtgttgtc ggggaagctg acgtcctttg caattcgata
    11041 tcaagctgta ccttctagat gtacatccca ataagcgctt ctagaccaat gacttacaag
    11101 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
    11161 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
    11221 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    11281 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11341 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
    11401 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
    11461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    11581 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    11641 attctccgcc ccatggctga ctaatttttt tt
//
