LOCUS       sequence-449069-       11537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcription factor DNMT1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11537)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-DNMT1-Brd 376
FEATURES             Location/Qualifiers
     source          1..11537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(122..138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(131..153)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      280..735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     577..598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        761..865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(866..934,935..1726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1084..1103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1897..2485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2386..2405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2639..2656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2809..2814,2815..2832,2833..2839)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2847..2863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2852..2874
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2871..2887
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2871..2887
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2906..2926
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4026..4259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4444..4488
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4906..5312
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    10487..10890
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     LTR             11009..11242
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11314..11435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11351..11370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11405..11424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(11475..11537,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagctgg ctgcagtaac gccattttgc
     4921 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     4981 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     5041 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     5101 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     5161 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     5221 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     5281 gcgccagtcc tccgacagac tgagtcggcc ggtcgaatca agcttatcga taccgtcgac
     5341 ggatccgcta gcccgcggtt cgaaatgccg gcgcgtaccg ccccagcccg ggtgcccaca
     5401 ctggccgtcc cggccatctc gctgcccgac gatgtccgca ggcggctcaa agatttggaa
     5461 agagacagct taacagaaaa ggaatgtgtg aaggagaaat tgaatctctt gcacgaattt
     5521 ctgcaaacag aaataaagaa tcagttatgt gacttggaaa ccaaattacg taaagaagaa
     5581 ttatccgagg agggctacct ggctaaagtc aaatcccttt taaataaaga tttgtccttg
     5641 gagaacggtg ctcatgctta caaccgggaa gtgaatggac gtctagaaaa cgggaaccaa
     5701 gcaagaagtg aagcccgtag agtgggaatg gcagatgcca acagcccccc caaacccctt
     5761 tccaaacctc gcacgcccag gaggagcaag tccgatggag aggctaagcg ttcaagagac
     5821 cctcctgcct cagcctccca agtaactggg attagagctg aaccttcacc tagccccagg
     5881 attacaagga aaagcaccag gcaaaccacc atcacatctc attttgcaaa gggccctgcc
     5941 aaacggaaac ctcaggaaga gtctgaaaga gccaaatcgg atgagtccat caaggaagaa
     6001 gacaaagacc aggatgagaa gagacgtaga gttacatcca gagaacgagt tgctagaccg
     6061 cttcctgcag aagaacctga aagagcaaaa tcaggaacgc gcactgaaaa ggaagaagaa
     6121 agagatgaaa aagaagaaaa gagactccga agtcaaacca aagaaccaac acccaaacag
     6181 aaactgaagg aggagccgga cagagaagcc agggcaggcg tgcaggctga cgaggacgaa
     6241 gatggagacg agaaagatga gaagaagcac agaagtcaac ccaaagatct agctgccaaa
     6301 cggaggcccg aagaaaaaga acctgaaaaa gtaaatccac agatttctga tgaaaaagac
     6361 gaggatgaaa aggaggagaa gagacgcaaa acgaccccca aagaaccaac ggagaaaaaa
     6421 atggctcgcg ccaaaacagt catgaactcc aagacccacc ctcccaagtg cattcagtgc
     6481 gggcagtacc tggacgaccc tgacctcaaa tatgggcagc acccaccaga cgcggtggat
     6541 gagccacaga tgctgacaaa tgagaagctg tccatctttg atgccaacga gtctggcttt
     6601 gagagttatg aggcgcttcc ccagcacaaa ctgacctgct tcagtgtgta ctgtaagcac
     6661 ggtcacctgt gtcccatcga caccggcctc atcgagaaga atatcgaact cttcttttct
     6721 ggttcagcaa aaccaatcta tgatgatgac ccatctcttg aaggtggtgt taatggcaaa
     6781 aatcttggcc ccataaatga atggtggatc actggctttg atggaggtga aaaggccctc
     6841 atcggcttca gcacctcatt tgccgaatac attctgatgg atcccagtcc cgagtatgcg
     6901 cccatatttg ggctgatgca ggagaagatc tacatcagca agattgtggt ggagttcctg
     6961 cagagcaatt ccgactcgac ctatgaggac ctgatcaaca agatcgagac cacggttcct
     7021 ccttctggcc tcaacttgaa ccgcttcaca gaggactccc tcctgcgaca cgcgcagttt
     7081 gtggtggagc aggtggagag ttatgacgag gccggggaca gtgatgagca gcccatcttc
     7141 ctgacaccct gcatgcggga cctgatcaag ctggctgggg tcacgctggg acagaggcga
     7201 gcccaggcga ggcggcagac catcaggcat tctaccaggg agaaggacag gggacccacg
     7261 aaagccacca ccaccaagct ggtctaccag atcttcgata ctttcttcgc agagcaaatt
     7321 gaaaaggatg acagagaaga caaggagaac gcctttaagc gccggcgatg tggcgtctgt
     7381 gaggtgtgtc agcagcctga gtgtgggaaa tgtaaagcct gcaaggacat ggttaaattt
     7441 ggtggcagtg gacggagcaa gcaggcttgc caagagcgga ggtgtcccaa tatggccatg
     7501 aaggaggcag atgacgatga ggaagtcgat gataacatcc cagagatgcc gtcacccaaa
     7561 aaaatgcacc aggggaagaa gaagaaacag aacaagaatc gcatctcttg ggtcggagaa
     7621 gccgtcaaga ctgatgggaa gaagagttac tataagaagg tgtgcattga tgcggaaacc
     7681 ctggaagtgg gggactgtgt ctctgttatt ccagatgatt cctcaaaacc gctgtatcta
     7741 gcaagggtca cggcgctgtg ggaggacagc agcaacgggc agatgtttca cgcccactgg
     7801 ttctgcgctg ggacagacac agtcctcggg gccacgtcgg accctctgga gctgttcttg
     7861 gtggatgaat gtgaggacat gcagctttca tatatccaca gcaaagtgaa agtcatctac
     7921 aaagccccct ccgaaaactg ggccatggag ggaggcatgg atcccgagtc cctgctggag
     7981 ggggacgacg ggaagaccta cttctaccag ctgtggtatg atcaagacta cgcgagattc
     8041 gagtcccctc caaaaaccca gccaacagag gacaacaagt tcaaattctg tgtgagctgt
     8101 gcccgtctgg ctgagatgag gcaaaaagaa atccccaggg tcctggagca gctcgaggac
     8161 ctggatagcc gggtcctcta ctactcagcc accaagaacg gcatcctgta ccgagttggt
     8221 gatggtgtgt acctgccccc tgaggccttc acgttcaaca tcaagctgtc cagtcccgtg
     8281 aaacgcccac ggaaggagcc cgtggatgag gacctgtacc cagagcacta ccggaaatac
     8341 tccgactaca tcaaaggcag caacctggat gcccctgagc cctaccgaat tggccggatc
     8401 aaagagatct tctgtcccaa gaagagcaac ggcaggccca atgagactga catcaaaatc
     8461 cgggtcaaca agttctacag gcctgagaac acccacaagt ccactccagc gagctaccac
     8521 gcagacatca acctgctcta ctggagcgac gaggaggccg tggtggactt caaggctgtg
     8581 cagggccgct gcaccgtgga gtatggggag gacctgcccg agtgcgtcca ggtgtactcc
     8641 atgggcggcc ccaaccgctt ctacttcctc gaggcctata atgcaaagag caaaagcttt
     8701 gaagatcctc ccaaccatgc ccgtagccct ggaaacaaag ggaagggcaa gggaaaaggg
     8761 aagggcaagc ccaagtccca agcctgtgag ccgagcgagc cagagataga gatcaagctg
     8821 cccaagctgc ggaccctgga tgtgttttct ggctgcgggg ggttgtcgga gggattccac
     8881 caagcaggca tctctgacac gctgtgggcc atcgagatgt gggaccctgc ggcccaggcg
     8941 ttccggctga acaaccccgg ctccacagtg ttcacagagg actgcaacat cctgctgaag
     9001 ctggtcatgg ctggggagac caccaactcc cgcggccagc ggctgcccca gaagggagac
     9061 gtggagatgc tgtgcggcgg gccgccctgc cagggcttca gcggcatgaa ccgcttcaat
     9121 tcgcgcacct actccaagtt caaaaactct ctggtggttt ccttcctcag ctactgcgac
     9181 tactaccggc cccggttctt cctcctggag aatgtcagga actttgtctc cttcaagcgc
     9241 tccatggtcc tgaagctcac cctccgctgc ctggtccgca tgggctatca gtgcaccttc
     9301 ggcgtgctgc aggccggtca gtacggcgtg gcccagacta ggaggcgggc catcatcctg
     9361 gccgcggccc ctggagagaa gctccctctg ttcccggagc cactgcacgt gtttgctccc
     9421 cgggcctgcc agctgagcgt ggtggtggat gacaagaagt ttgtgagcaa cataaccagg
     9481 ttgagctcgg gtcctttccg gaccatcacg gtgcgagaca cgatgtccga cctgccggag
     9541 gtgcggaatg gagcctcggc actggagatc tcctacaacg gggagcctca gtcctggttc
     9601 cagaggcagc tccggggcgc acagtaccag cccatcctca gggaccacat ctgtaaggac
     9661 atgagtgcat tggtggctgc ccgcatgcgg cacatcccct tggccccagg gtcagactgg
     9721 cgcgatctgc ccaacatcga ggtgcggctc tcagacggca ccatggccag gaagctgcgg
     9781 tatacccacc atgacaggaa gaacggccgc agcagctctg gggccctccg tggggtctgc
     9841 tcctgcgtgg aagccggcaa agcctgcgac cccgcagcca ggcagttcaa caccctcatc
     9901 ccctggtgcc tgccccacac cgggaaccgg cacaaccact gggctggcct ctatggaagg
     9961 ctcgagtggg acggcttctt cagcacaacc gtcaccaacc ccgagcccat gggcaagcag
    10021 ggccgcgtgc tccacccaga gcagcaccgt gtggtgagcg tgcgggagtg tgcccgctcc
    10081 cagggcttcc ctgacaccta ccggctcttc ggcaacatcc tggacaagca ccggcaggtg
    10141 ggcaatgccg tgccaccgcc cctggccaaa gccattggct tggagatcaa gctttgtatg
    10201 ttggccaaag cccgagagag tgcctcagct aaaataaagg aggaggaagc tgctaaggac
    10261 tagaccggtc ggatcccccg ggctgcagga attcgagcat cttaccgcca tttattccca
    10321 tatttgttct gtttttcttg atttgggtat acatttaaat gttaataaaa caaaatggtg
    10381 gggcaatcat ttacattttt agggatatgt aattactagt tcaggtgtat tgccacaaga
    10441 caaacatgtt aagaaacttt cccgttattt acgctctgtt cctgttaatc aacctctgga
    10501 ttacaaaatt tgtgaaagat tgactgatat tcttaactat gttgctcctt ttacgctgtg
    10561 tggatatgct gctttaatgc ctctgtatca tgctattgct tcccgtacgg ctttcgtttt
    10621 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtccg
    10681 tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct ggggcattgc
    10741 caccacctgt caactccttt ctgggacttt cgctttcccc ctcccgatcg ccacggcaga
    10801 actcatcgcc gcctgccttg cccgctgctg gacaggggct aggttgctgg gcactgataa
    10861 ttccgtggtg ttgtcgggga agctgacgtc ctttgcaatt cgatatcaag ctgtaccttc
    10921 tagatgtaca ttacagaaag cgcttctaga ccaatgactt acaaggcagc tgtagatctt
    10981 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
    11041 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
    11101 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
    11161 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
    11221 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
    11281 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
    11341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    11401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
    11461 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    11521 gctgactaat ttttttt
//
