LOCUS       sequence-44908-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS282
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(268..287)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     537..555
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      697..706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    714..766
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
       61 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
      121 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
      181 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
      241 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
      301 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
      361 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
      421 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
      481 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
      541 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
      601 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
      661 tgatgatggc catgttatcc tcctcgccct tgctcaccat ggtggcgacc ggtggatccc
      721 gggcccgcgg taccgtcgat tgcagaattc gaagcttgag ctcgagtcta
//
