LOCUS       sequence-44910-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mid2(SS/TM), containing only the signal sequence and 
            transmembrane helix, fused to GFP and LOVpep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS257
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(498..518)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tatccaccgc tttatcaatc tcttctgcag ttttcttaat cagcatgact gcctctctct
       61 cggcagcatc tcggacatgc tcagttccat ccaactgaac cccaataaag tactggacat
      121 ctcccttctg atctcgcata ggctgcaagt gaaagaggtt ccagaacttt ttaccactct
      181 ttgtataatt aatcagctga acagtgacct ctgtttggtt atctatggca tctctaattt
      241 ttctcactgt cgcgcgatca gtttcaggac cttgtagaaa cctgcagttt cttcccaaaa
      301 tttcttcacg gctatattct gtcaactgca agaaactatc ggacgcgaat ataatgggat
      361 tatctggcaa tcttgggtca gtaatgacaa agttcttctc aatacgttca agtgtagtag
      421 ccaacctagg acttccacct gaacctccag atccaccttt gtatagttca tccatgccat
      481 gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg
      541 gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc
      601 catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa
      661 tgttgtgtct aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt
      721 atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt
      781 ta
//
