LOCUS       sequence-44913-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a low-affinity ePDZ variant fused to mCherry in yeast. 
            Illumination with 440 nm light induces binding to LOVpep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS220
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    118..170
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggtggc gaccggtgga
      121 tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag tctagaggta
      181 cggtagttaa tcgagattgg gctagagtag taatggtagt tgtaatgtgc gtatacagtg
      241 atggtatagt cgacacccgg tttcaggccg ctgatggtag cagtagactt ggaaccaggt
      301 acagtgaatt cctgaaccgg ggagttacca ccggtttcac cgtacgtgat acggtaataa
      361 gaaacatgag agtcgtagta cgcatcccag ctgatcagca ggctagtcgg ggtcgcagca
      421 acaacttcca gattggtcgg aacagaagaa acgcctccag aaccaccgtc tttttcaacc
      481 ctcactcgaa tctcttgttt tgcaccgtta ccaacttctc gtacaatgat gagttcaact
      541 gtattctgga aagtttttag caaggacact gcttgtccat gttcaatatt tataaaactg
      601 tagccattag cctgaataat tttatcacct ggctgcagta attttgatgc tggtccttca
      661 ggttgtaccc ttgttacaaa tataccatca tcatcaggtc tgaatgggtt tcctctaccc
      721 ccgacaccac ctgatatgct aaatccaagt tctggcattg tcgacggtat cgataagctt
//
