LOCUS       sequence-44914-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a high-affinity ePDZ variant fused to mCherry in yeast. 
            Illumination with 440 nm light induces binding to LOVpep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, 
            Sosnick TR, Weiss EL, Glotzer M
  TITLE     TULIPs: tunable, light-controlled interacting protein tags for cell 
            biology.
  JOURNAL   Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904.
  PUBMED    22388287
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904."
COMMENT     Sequence Label: pDS221
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(260..279)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     529..547
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      689..698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    706..758
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat ggtgtagtcc
       61 tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg cagctgcacg
      121 ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc gtccttcagc
      181 ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat ccgctcggag
      241 gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg gaagttggtg
      301 ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg
      361 gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag
      421 gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac
      481 atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt ggtcaccttc
      541 agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac
      601 tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc cttgatgatg
      661 gccatgttat cctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc
      721 ggtaccgtcg actgcagaat tcgaagcttg agctcgagtc tagaggtacg g
//
