LOCUS       sequence-449141-        3687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  multiple resistance marker with apramycin and kanamycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3687)
  TITLE     pSuiAb::Apr+KM
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSuiAb
FEATURES             Location/Qualifiers
     source          1..3687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(464..1264)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     primer_bind     complement(1436..1452)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1436..1452)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1511..1527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1511..1527)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1524..1546)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1535..1551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1559..1565,1566..1583,1584..1589))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1604..1625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1742..1759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1913..2501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1993..2012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2672..3487)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2672..3487)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3395..3414
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3488..3592)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3660..3678
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gcctcgaggg tcatgcatgc atatccatgg ttagcggtct
      121 gcttcttcca gccctcctgt ttgaagatgg caagttagtt acgcacaata aaaaaagacc
      181 taaaatatgt aaggggtgac gccaaagtat acactttgcc ctttacacat tttaggtctt
      241 gcctgcttta tcagtaacaa acccgcgcga tttacttttc gacctcattc tattagactc
      301 tcgtttggat tgcaactggt ctattttcct cttttgtttg atagaaaatc ataaaaggat
      361 ttgcagacta cgggcctaaa gaactaaaaa atctatctgt ttcttttcat tctctgtatt
      421 ttttatagtt tctgttgcat gggcataaag ttgcaagctt tcagccaatc gactggcgag
      481 cggcatcgca ttcttcgcat cccgcctctg gcggatgcag gaagatcaac ggatctcggc
      541 ccagttgacc cagggctgtc gccacaatgt cgcgggagcg gatcaaccga gcaaaggcat
      601 gaccgactgg accttccttc tgaaggctct tctccttgag ccacctgtcc gccaaggcaa
      661 agcgctcaca gcagtggtca ttctcgagat aatcgacgcg taccaacttg ccatcctgaa
      721 gaatggtgca gtgtctcggc accccatagg gaacctttgc catcaactcg gcaagatgca
      781 gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac ctgcccatcg agttcatgga
      841 cacgggcgac cgggcttgca ggcgagtgag gtggcagggg caatggatca gagatgatct
      901 gctctgcctg tggccccgct gccgcaaagg caaatggatg ggcgctgcgc tttacatttg
      961 gcaggcgcca gaatgtgtca gagacaactc caaggtccgg tgtaacgggc gacgtggcag
     1021 gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat gacgagcgtc cctcccggac
     1081 ccagcgcagc acgcagggcc tcgatcagtc caagtggccc atcttcgagg ggccggacgc
     1141 tacggaagga gctgtggacc agcagcacac cgccgggggt aaccccaagg ttgagaagct
     1201 gaccgatgag ctcggctttt cgccattcgt attgcacgac attgcactcc accgctgatg
     1261 acatcagtcg atcatagcac gatcaacggc actgttgcaa atagtcggtg gtgataaact
     1321 tatcatcccc ttttgctgat ggagctgcac atgaaccgga tccgtgaatt gacgcgtatt
     1381 gggatggtac cgggcccccc ctcgaggtcg acgatagaat tcctgcagcc cgggggatcc
     1441 actagttcta gagcggccgc caccgcggtg gagctcatcc caatggcgcg ccgagcttgg
     1501 ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2341 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2641 aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa
     2701 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     2761 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     2821 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2881 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     2941 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     3001 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     3061 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     3121 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     3181 cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     3241 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     3301 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     3361 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     3421 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     3481 ggctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3541 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3601 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3661 ataggcgtat cacgaggccc tttcgtc
//
