LOCUS       sequence-449142-        7397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  promoter activity study.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Qin H, Sun Y, Benveniste EN
  TITLE     The transcription factors Sp1, Sp3, and AP-2 are required for 
            constitutive matrix metalloproteinase-2 gene expression in 
            astroglioma cells.
  JOURNAL   J Biol Chem. 1999 Oct 8;274(41):29130-7.
  PUBMED    10506168
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1999-10-8"; volume: "274(41)"; pages: "29130-7"
COMMENT     Sequence Label: human MMP-2 promoter
FEATURES             Location/Qualifiers
     source          1..7397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    88..209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     RBS             1830..1841
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2109..3761
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2175..2193)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          4002..4067
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4197..4217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4642..4776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4918..4935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5089..5677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5169..5188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5848..6639,6640..6708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6471..6490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6709..6813)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6840..7295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6927..6946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7137..7158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtacgtgg aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
       61 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      181 ggtttgtcca aactcatcaa tgtatcttat ggtactgtaa ctgagctaac ataacccggg
      241 aggtaccgag ctcttacgcg tgctagctcg agcggccgcc agtgtgatgg atatctgcag
      301 aattcggctt cacacccacc agacaagcct gaacttgtct gaagcccact gagacccaag
      361 ccgcagagac ttttctagct gtgatgatca agacataatc atgacctcca atgcccccca
      421 caagtatatt gctcctgatt ctttcagccc ctgaccttac ttctcaaact gttccctgct
      481 gacccccagt cctatctgcc cccttcctag gctggtcctt actgacccct ccagctccat
      541 cccctcaccc tgtgccccac ctttttcaga tagaaaaaac tttcttctcc agtgcctctt
      601 gctgtttttc atctctgggc cattgtcaat gttccctaaa acattcccca tattccccac
      661 ccagcactct acctctttag ctcttcaggt ctcagctcag aagtcacttc ttccaggaag
      721 ccttccttga ttgtctttac tagtttaggg gctgaagtca ggcgttccca acagcctgct
      781 ggagttcccc atcacagctt atctctcaac tgtctttcct gagagaggga gaagacattc
      841 ctcagagacg gttgtcacag ggagaacttc aaaattggga ttcgacctga gaggccacat
      901 ggattcttgg cttggcgcag gaaaggattc aagagtgagt ggggaattcg tggaactgag
      961 ggctcctccc ctttttagac catatagggt aaacctcccc acattgccat ggcatttata
     1021 aactgccatg gcactggtgg gtgcttcctt taacatgcta atgcattata attagcgtaa
     1081 aatgagcagt gaggatgacc agaggtcgct ttctttgcca tcttggtttt ggctggcttc
     1141 ttcactgcat actgttttat cagtggggtc tttgtgacct ctatcgtatt aaaccagtct
     1201 tgcccaattt ctatctcatc ctgtgaccga gaatgcggac cctcctggga gtgcagccca
     1261 gcaggtctca gcctcatttt acccagcccc ctgttcaaga tggagtcgct ctggttccaa
     1321 cgtctctaac gcggggcccc tgactgctct atttcccaag gtgtatctag catctcgcgc
     1381 tatacgaggc caagttaagg cttacacatt tgcagaagga aagaggtaag gaagcaacct
     1441 gggaccttcc actgtctctg tttccatctc tctctttcca tctctgttca tcccagaatc
     1501 tctctgtccc tatccctaaa tatcgaaaat ttctgtctct gaccatctat cattgtggct
     1561 gatcatctgt ttctgaccat tccttcccgt tcctgacccc agggagtgca gggtgtccta
     1621 gccaagccgg cgtccctcct agtagtaccg ctgctctcta acctcaggac gtcaagggcc
     1681 tagagcgaca gatgtttccc agcagggggt tctgaggctg tgcgcccaga tcgcgagaga
     1741 ggcaagtggg gtgacgaggt cgtgcactga gggtggacgt agaggccagg agtagcaggc
     1801 ggccggggaa aagaggtgga gaaaggaaaa aagaggagaa aagtggagga gggcgagtag
     1861 gggggtgggg cagagagggg cgggcccgag tgcgcccccc gcccccagcc ccgctctgcc
     1921 agctccctcc cagcccagcc ggctacatct ggcggctgcc ctcccttgtt tccgctgcat
     1981 ccagacttcc tcaggcggtg gctggaggct gcgcatctgg ggcttaagcc gaattccagc
     2041 acactggcgg ccgttactag tggatccgag ctcggtacca agcttggcat tccggtactg
     2101 ttggtaaaat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
     2161 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     2221 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
     2281 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     2341 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     2401 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     2461 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
     2521 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
     2581 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     2641 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
     2701 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
     2761 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     2821 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     2881 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
     2941 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
     3001 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
     3061 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     3121 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     3181 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     3241 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     3301 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     3361 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct
     3421 tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat
     3481 tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg
     3541 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     3601 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     3661 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     3721 tcctcataaa ggccaagaag ggcggaaagt ccaaattgta aaatgtaact gtattcagcg
     3781 atgacgaaat tcttagctat tgtaatactg cgatgagtgg cagggcgggg cgtaattttt
     3841 ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa
     3901 gcggatgaat ggcagaaatt cgccggatct ttgtgaagga accttacttc tgtggtgtga
     3961 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     4021 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     4081 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     4141 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     4201 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     4261 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     4321 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     4381 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     4441 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     4501 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     4561 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     4621 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     4681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4741 tccaaactca tcaatgtatc ttatcatgtc tggatccgtc gaccgatgcc cttgagagcc
     4801 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     4861 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
     4921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6841 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6901 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6961 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7021 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7081 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7141 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7201 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7261 cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa
     7321 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg
     7381 ataagtaagt aatatta
//
