LOCUS       sequence-449145-        7912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing LGC-47 in AIB neuron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Kumar S, Sharma AK, Leifer AM
  TITLE     An inhibitory acetylcholine receptor gates context dependent 
            mechanosensory processing in C. elegans.
  JOURNAL   bioRxiv [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204.
  PUBMED    38585821
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Mar 27:2024.03.21.586204. doi: 
            10.1101/2024.03.21.586204."
COMMENT     Sequence Label: pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3...
FEATURES             Location/Qualifiers
     source          1..7912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5334..5356)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5456..5475
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5553..5571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5639..5743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5744..5812,5813..6604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5962..5981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6775..7363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7264..7283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7517..7534
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7651..7672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7687..7692,7693..7710,7711..7717)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7725..7741
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7730..7752
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7749..7765
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7749..7765
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cttaactggc accgtatatt gcacacttaa gaaaaaatat aaaaaaaact gaaatattat
       61 ataaaaatat catttttttt tcaatctgca tcgtgttcaa ttcctgaata atcagtaaat
      121 agcaagtata tgtatcggtt tttaattgac attttagttt ctgttcttaa gctgaacaag
      181 ttgtttttaa cgcattgatt aaaattttgt catgatccgt aataaagtcc aacgccattt
      241 aaaaggtttc tgaagaaatt gttatttttt cttctttttt ttcaattgat tttcaatatg
      301 atgagtacaa gtacgtgatg aattataaca ctcttattac aaaatacaag actttttact
      361 acttctcatt ctatccggcg cctaatggta catctagata tgtgccctcc ggcgactcac
      421 agaatccagt aaacacaact ctgacactga caattcctgt ttcgttcgca acacacgtca
      481 ggattgctat ttccgttaaa gtaacaaatg tcactttttc ttagatttct tccgtctatt
      541 gatatagatt ttagttttac tattagctca acaatttttt tagaaaaaaa agtactgaat
      601 taaaaaaaaa ccacaaaatt gaaagttcaa actttcgact tatgcatttc gttatgcatt
      661 tttgacactt ttttaccggc gggacaatgg agtttcacac ttttgcgagg aatgcataac
      721 ttgaataact aacaaggttt ctaaaaactt ggttcggaaa acggaaataa agaaactact
      781 ttacaagtcg catttttgat aaatctagaa cgctctattg tgctgtcttg atatgtgttt
      841 ttccaaattt aatgtacttt gtaggtttat tcgtctttat tgtaatttta gagctacttc
      901 ctgggaaatg tcgttgggaa atgtcatcat tgcctcttca gtaaatacac gttcacccat
      961 aggtgtataa ttgaacttat gtatatgttt tttcctattc gcaattgtta cttcccgctt
     1021 ttttccattt ctggatatct attacgatgc caaattggat cagagggtcc ctgacacata
     1081 cacaaacgtc ccggtgttgc tctttcttta agttcttctc ctatgcttct tcctttccta
     1141 ttctccacca tctggagcgt agtcacgtcg ggcatacagc aatactgact gcgtgcttaa
     1201 ttgaaacatc aaacagattc acatttgatt tgcgccccat ctaaaagaga tgcacactaa
     1261 tccaattttt tgtctatatc gatgtctttc tactgtgtga aggagcagtt ttgcgctccg
     1321 tttcgcgtaa aggaaaagga aaaagggagg cagtgctcaa tacacggcta ctataaaaac
     1381 aagtgaaaga ctaaagaggc ggcacacaac agtgtcgaaa aggggttgaa agaagaagga
     1441 tgggggtgaa gaagaagaac aattattttt gtgctgacat gagatttttg cgtattgact
     1501 gacttgacta ttttccgaca atatttaacc ttgggatatt ttgcgacatc tgggtctgca
     1561 ggctctgtgt ccgctggtcc atctcatcca attccatcaa attcgcagct cttcttccat
     1621 ttttctcact gaaaccatca tccatcgtat gcacattaaa ctcccccgcg tgatttcact
     1681 caatcacttt actcactcca tttcagcaaa tccatctcat tttgaaaaag tgaaatgaaa
     1741 agcgaatacg tcaatctttg aggaagaatt tcgtccctct catttttcaa tccttcttcg
     1801 tcccccaacg gacggtttct aagcgatatg gctcataact catcgggcca actcttacgt
     1861 tgggaaaaat aagatttatt ccctcaactc gaaatgtttt gttcctcctt ttctcaattc
     1921 tcattttctt agtcaccttg gttactttag ctttatgaat gtatttttta taatacaaat
     1981 atatcttaca gttcgctttc atatcttcta aattcttttg aaatcatcaa ctttttgaca
     2041 ggatccccgg gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat
     2101 agaacatttt caggaggacc cttggagggt accgagctca gaaaaaatgc tctccacatt
     2161 gtttctgttg ctctatgcca cccgggaaac ggtcaatgca atgactgccg agatcagccc
     2221 aactgtttct tatttattag atcgaggttg tgcaaatgat acggaaatta ttgatcatct
     2281 tcttatcgac cgtgctctat actacaataa acataagcta ccatctccac aagtagacgt
     2341 gaggattgag atgtgggtac aagaagtgac atcagtttcc gagctaacgc aagattttga
     2401 aatagattta tacatgaacg aatattggac agaccccggg ttagcctacg acattctcaa
     2461 tccttgtcag ggcaatctat catttgattg ggctgtcatg cagaatatct ggactccaaa
     2521 cacatgcttc atcaattcca aaaaggcaca acttcacagc tcgccattta ctaatgtttt
     2581 cttaatggtc tttccaaatg gctcagtgtg gtcaaattgg aggataaaga gtacgggccc
     2641 ttgtgtgatg gatctgacaa agtttccaat ggacagtatc gaatgtacac tgacttttga
     2701 atcattcaac tacaacaagg acgaagtttt catgcgatgg ggcgatcctc cattaacgat
     2761 cttcaaaccc atcgaactac ctgatttcac aatgacaaac tttagtactt caaataaatt
     2821 tcagctttat gctgccggct actggagtga actcaatgtg aactttctct tccgccgaag
     2881 gtctggatgg tatctcttgc aaggttacat tccaacctat atgacagtgt tcatttcttg
     2941 gattccattt tacttgggcc caaaagcaat acccgctcgg actatgatag gtgtcaatgc
     3001 cctactggcg cttaccttcc agtttggtaa tataatccga aacctaccac gagtgtcgta
     3061 tatcaaagcc atcgacgtct ggatgttgag tggcatatgc tttgtttttg catctttgtt
     3121 tgaacttgca accatcggtt ttttgatgcg aaatgaaggc aaaccggcga caaaatcaag
     3181 ccgttcaact aaaaattcta atcgttggca acaaccaagt gtttgccgag aagacactca
     3241 aagaaagtca caaaatatcg tattctgtga gcgattagac aacacgtcaa gattaatgtt
     3301 tccagtagtg ttcacattct acaacttcat atattggttt gtttacatga aacaagcatg
     3361 ggatcaggca ccataaggta ccgctgtctc atcctacttt cacctagtta actgcttgtc
     3421 ttaaaatcta tgcttctctt tagtatctaa aattttccta gaagcttaca agtatataaa
     3481 tggtctcttc tcaataaagg ttgtatattt attcatctta ttgaatctgc catttcctcg
     3541 tttttgcgag tttatatacc ttccaatttt ctttctattg tattttcaac ttctaatttt
     3601 aattcaggga aactgcttca acgcatcatg tccgaactca tcaaggagaa catgcacatg
     3661 aagctctaca tggagggaac cgtagacaac caccacttca agtgcacctc cgagggagag
     3721 ggaaagccat acgagggaac ccaaaccatg cgtatcaagg tcgtcgaggg aggaccactc
     3781 ccattcgcct tcgacatcct cgccacctcc ttcctctacg ggtccaaggt aagtttaaac
     3841 atatatatac taactaaccc tgattattta aattttcaga ccttcatcaa ccacacccaa
     3901 ggaatcccag acttcttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcacc
     3961 acctacgagg acggaggagt cctcaccgcc acccaagaca cctccctcca agacggatgc
     4021 ctcatctaca acgtcaagat ccgtggagtc aacttcacct ccaacggacc agtcatgcaa
     4081 aagaagaccc tcggatggga gaccttcacc gagaccctct acccagccga cggaggacta
     4141 gagggacgta acgacatggc cctcaagctc gtcggaggct cccacctcat cgccaacatc
     4201 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagaccacc
     4261 taccgttcca agaagccagc caagaacctc aagatgccag gagtctacta cgttgactac
     4321 cgtctagagc gtatcaagga ggccaacaac gagacctacg tcgagcaaca cgaggtcgcc
     4381 gtcgcccgtt actgcgacct cccatccaag ctcggacaca agctcaacta agcgccggtc
     4441 gctaccatta ccaacttgtc tggtgtcaaa aataataggg gccgctgtca tcagagtaag
     4501 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagcatc tcgcgcccgt
     4561 gcctctgact tctaagtcca attactcttc aacatcccta catgctcttt ctccctgtgc
     4621 tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg ttttttagct
     4681 tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa
     4741 taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc
     4801 tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt ttttgaaaca
     4861 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     4921 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     4981 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     5041 tgcttttgct ttttggggtt tcccctattg tttgtcaaga tttcgaggac ggcgtttttc
     5101 ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt
     5161 ttgaggctca gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg
     5221 ggtttttgcc ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact
     5281 agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     5341 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     5401 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     5461 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     5521 aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     5581 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     5641 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5701 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5761 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5821 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5881 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5941 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6001 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6061 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6121 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6181 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6241 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6301 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6361 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6421 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6481 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6541 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6601 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6661 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7261 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7321 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7381 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7441 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7501 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7561 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7621 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7681 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7741 atttcacaca ggaaacagct atgaccatga ttacgccaag ctgtaagttt aaacatgatc
     7801 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
     7861 aacgatggat acgctaacaa cttggaaatg aaataagctt gcatgcctgc ag
//
