LOCUS       sequence-449169-        3896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that 
            lacks all methionines fused with the full-length recoded NSP5 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1noMSA11-NSP5(io)
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(93..161,162..953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(311..330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1124..1712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1613..1632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1866..1883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1968..1987
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1968..1986
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2953..2971)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2967..3014
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(3082..3100)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3083..3100)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3390..3409)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3619..3638)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3738..3760
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3798..3816)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(3884..3896,1..92)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
       61 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
      961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     1921 ccgcgcgttg gccgattcat taatgcaggg ggatatcgat cccgggttaa tacgactcac
     1981 tatagggctt ttttttgaaa agtcttgtgt tagccaaggc tacttttaaa gaagcaagct
     2041 ttcattatcg tagattaact gctttaaatc ggagattaag caacattggt gcaaattcta
     2101 tttggaagcc agttcctgcc atgagtttaa gtatagatgt tacatcactt ccctcgatcc
     2161 cctcaacaat atataaaaat gaaagttcaa gtacaacaag tacactatcc ggaaaatcta
     2221 taggaagaag tgaacaatat ataagcccag atgcagaagc ttttaataaa tatatgctaa
     2281 gtaaatcacc tgaagatata gggccaagtg atagtgcatc aaacgatccc ctcacaagtt
     2341 tttcaatacg aagtaacgca gtaaaaacta atgccgatgc aggtgtaagc atggactcct
     2401 cagcacaatc aagaccttca tctaacgtag gatgtgatca agtcgatttc tcattaaaca
     2461 aaggattaaa agttaaagca aatttagatt catctataag tataagcaca gatacaaaaa
     2521 aagaaaaaag taaacaaaat cataaaagtc gaaaacacta tccaagaata gaagccgaaa
     2581 gtgatagcga tgattatgta ctagacgaca gcgattcaga tgatggaaaa tgtaaaaatt
     2641 gcaaatacaa aaaaaaatat tttgcactaa gaatgagaat gaaacaagtt gccatgcaat
     2701 taattgaaga tttataaaat tatgtcacta tctaattata cagtatttag ccatcacaag
     2761 accgtccaga ctagagtagc gcctagctgg caaaatactg tgaaccgggt cggcatggca
     2821 tctccacctc ctcgcggtcc gacctgggca tccgaaggag gacgtcgtcc actcggatgg
     2881 ctaagggaga gctcaaaaaa aaggatccgg ctgctaacaa agcccgaaag gaagctgagt
     2941 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3001 tgaggggttt tttgctgaaa ggaggaacta tatccggatc gagatcctct agagtcgacc
     3061 tgcaggcatg caagcttgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     3121 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     3181 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     3241 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     3301 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     3361 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     3421 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     3481 cgtggccggg ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc
     3541 aacggcctca acctactact gggctgcttc ttaatgcagg agtcgcataa gggagagcgt
     3601 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3661 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3721 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3781 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3841 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa ccccta
//
