LOCUS       sequence-449171-        3896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that 
            lacks all methionines fused with the full-length SA11 NSP5 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  TITLE     Rotavirus Nonstructural Protein (NSP) plasmids
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-35aaNSP1noMSA11-NSP5(wt)
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     155..172
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     257..276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        257..275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(436..447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1242..1260)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1256..1303
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(1371..1389)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1372..1389)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1679..1698)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1908..1927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2027..2049
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2087..2105)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2173..2277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2278..2346,2347..3138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2496..2515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3309..3896,1)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3798..3817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagggg
      241 gatatcgatc ccgggttaat acgactcact atagggcttt tttttgaaaa gtcttgtgtt
      301 agccaaggct acttttaaag aagcaagctt tcattatcgt agattaactg ctttaaatcg
      361 gagattaagc aacattggtg caaattctat ttggaagcca gttcctgcca tgtctctcag
      421 tattgacgtg acgagtcttc cttctattcc ttcaactata tataagaatg aatcgtcttc
      481 aacaacgtca actctttctg gaaaatctat tggtaggagt gaacagtaca tttcaccaga
      541 tgcagaagca ttcaataaat acatgctgtc gaagtctcca gaggatattg gaccatctga
      601 ttctgcttca aacgatccac tcaccagttt ttcgattaga tcgaatgcag ttaagacaaa
      661 cgcagacgct ggcgtgtcta tggattcatc agcacaatca cgaccttcaa gtaatgtcgg
      721 atgcgatcaa gtggatttct ccttaaataa aggcttaaaa gtaaaagcta atttggactc
      781 atcaatatca atatctacgg atactaaaaa ggagaaatca aaacaaaacc ataaaagtag
      841 gaagcactac ccaagaattg aagcagagtc tgattcagat gattatgtac tggatgattc
      901 agatagtgat gatggtaaat gtaagaactg taaatataag aagaaatact tcgcattaag
      961 aatgagaatg aaacaagtcg caatgcaatt gattgaagat ttgtaaaatt atgtcactat
     1021 ctaattatac agtatttagc catcacaaga ccgtccagac tagagtagcg cctagctggc
     1081 aaaatactgt gaaccgggtc ggcatggcat ctccacctcc tcgcggtccg acctgggcat
     1141 ccgaaggagg acgtcgtcca ctcggatggc taagggagag ctcaaaaaaa aggatccggc
     1201 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     1261 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
     1321 atccggatcg agatcctcta gagtcgacct gcaggcatgc aagcttgtat tctatagtgt
     1381 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     1441 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     1501 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     1561 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     1621 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     1681 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     1741 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     1801 ttgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttct
     1861 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     1921 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1981 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2041 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2101 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2161 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2221 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2281 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2341 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2401 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2461 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2521 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2581 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2641 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2701 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2761 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2821 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2881 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2941 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3001 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3061 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3121 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3181 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3241 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3361 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3421 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3481 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3541 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3601 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3661 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3721 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3781 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3841 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaa
//
