LOCUS       sequence-449192-        8167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Promoterless bicistronic luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM
  TITLE     The integrated stress response drives MET oncogene overexpression in
            cancers.
  JOURNAL   EMBO J. 2025 Jan 7. doi: 10.1038/s44318-024-00338-4.
  PUBMED    39774381
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2025 Jan 7. doi: 10.1038/s44318-024-00338-4."
COMMENT     Sequence Label: RLuc_IRES_FLuc_Pless
FEATURES             Location/Qualifiers
     source          1..8167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..941
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1753..3405
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1819..1837)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(3488..3506)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3489..3506)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3526..3543)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3532..3756
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3802..4230
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3889..3908)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4099..4120
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4239..4259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4244..4573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4424..4559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4486..4505
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4640..5434
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4694..4713)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5304..5323
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5608..5729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5645..5664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5699..5718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5778..5794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5778..5794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5791..5813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5802..5818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5826..5832,5833..5850,5851..5856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5871..5892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6009..6026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6180..6768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6260..6279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6939..7730,7731..7799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7562..7581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7800..7904)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7979..7998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccaccatgac ttcgaaagtt tatgatccag aacaaaggaa acggatgata actggtccgc
       61 agtggtgggc cagatgtaaa caaatgaatg ttcttgattc atttattaat tattatgatt
      121 cagaaaaaca tgcagaaaat gctgttattt ttttacatgg taacgcggcc tcttcttatt
      181 tatggcgaca tgttgtgcca catattgagc cagtagcgcg gtgtattata ccagacctta
      241 ttggtatggg caaatcaggc aaatctggta atggttctta taggttactt gatcattaca
      301 aatatcttac tgcatggttt gaacttctta atttaccaaa gaagatcatt tttgtcggcc
      361 atgattgggg tgcttgtttg gcatttcatt atagctatga gcatcaagat aagatcaaag
      421 caatagttca cgctgaaagt gtagtagatg tgattgaatc atgggatgaa tggcctgata
      481 ttgaagaaga tattgcgttg atcaaatctg aagaaggaga aaaaatggtt ttggagaata
      541 acttcttcgt ggaaaccatg ttgccatcaa aaatcatgag aaagttagaa ccagaagaat
      601 ttgcagcata tcttgaacca ttcaaagaga aaggtgaagt tcgtcgtcca acattatcat
      661 ggcctcgtga aatcccgtta gtaaaaggtg gtaaacctga cgttgtacaa attgttagga
      721 attataatgc ttatctacgt gcaagtgatg atttaccaaa aatgtttatt gaatcggacc
      781 caggattctt ttccaatgct attgttgaag gtgccaagaa gtttcctaat actgaatttg
      841 tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc tgatgaaatg ggaaaatata
      901 tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata attctagagc ggcccccaag
      961 cttgggctgc aggtcttaaa acagctctgg ggttgtaccc accccagagg cccacgtggc
     1021 ggctagtact ccggtattgc ggtacctttg tacgcctgtt ttatactccc ttcccccgta
     1081 acttagaagc acaatgtcca agttcaatag gagggggtac aaaccagtac caccacgaac
     1141 aagcacttct gttcccccgg tgaggctgta taggctgttt ccacggctaa aagcggctga
     1201 tccgttatcc gctcatgtac ttcgagaagc ctagtatcac cttggaatct tcgatgcgtt
     1261 gcgctcaaca ctcaacccca gagtgtagct taggtcgatg agtctggacg ttcctcaccg
     1321 gcgacggtgg tccaggctgc gttggcggcc tacctgtggc ccaaagccac aggacgctag
     1381 ttgtgaacaa ggtgtgaaga gcctattgag ctacctgaga gtcctccggc ccctgaatgc
     1441 ggctaatcct aaccacggag caggcagtgg caatccagcg accagcctgt cgtaacgcgc
     1501 aagttcgtgg cggaaccgac tactttgggt gtccgtgttt ccttttattt ttacaatggc
     1561 tgcttatggt gacaatcatt gattgttatc ataaagcaaa ttggattggc catccggtga
     1621 gaatttgatt attaaattac tctcttgttg ggattgctcc tttgaaatct tgtgcactca
     1681 cacctattgg aattacctca ttgttaagat atccatcaca ctggcggccg catctagagg
     1741 gcccggatcc aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatcct
     1801 ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     1861 cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac
     1921 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     1981 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     2041 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     2101 agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt
     2161 ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg
     2221 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     2281 aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg
     2341 aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc
     2401 gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     2461 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     2521 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc
     2581 cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc
     2641 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc
     2701 gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata
     2761 cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     2821 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     2881 gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg
     2941 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     3001 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catagttgac
     3061 cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg
     3121 atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc cgacgatgac
     3181 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     3241 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     3301 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     3361 gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaaatgt aactgtattc
     3421 agcgatgacg aaattcttag ctattgtaat cctccgaggc ctcgagcatg catctagagg
     3481 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     3541 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3601 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3661 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3721 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3781 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3841 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3901 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3961 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4021 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4081 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4141 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4201 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4261 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4321 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4381 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4441 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4501 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4561 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4621 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4681 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4741 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4801 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4861 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4921 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4981 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5041 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5101 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     5161 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     5221 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     5281 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     5341 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     5401 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     5461 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5521 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5581 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5641 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5701 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5761 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5821 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5881 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5941 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6001 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6061 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6121 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6181 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6241 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6301 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6361 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6421 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6481 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6541 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6601 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6661 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6721 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6781 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6841 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7921 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     7981 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     8041 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     8101 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     8161 atacgcg
//
