LOCUS       sequence-449210-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mPapaya, for MuSIC barcode construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, 
            Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR
  TITLE     Genetically Encoded Fluorescence Barcodes Allow for Single-Cell 
            Analysis via Spectral Flow Cytometry.
  JOURNAL   ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 
            10.1021/acssynbio.4c00807. Epub 2025 May 6.
  PUBMED    40326708
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00807"; journalName: "ACS Synth Biol"; date: 
            "2025-05-16- 16"; volume: "14"; issue: "5"; pages: "1533-1548"
COMMENT     Sequence Label: pR-mPapaya
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 atggtgagca agggcgaggg gcaatcgaag catggactaa aggaggagat gacggtgaag
       61 taccacatgg aaggttgcgt taatggtcac aaatttgtca ttactggaga gggcattgga
      121 aaccctttta agggtaagca aaccgcaaat ttgtgtgtga tagaaggagg cccgctgccg
      181 ttctcggagg acattttaag tccgggtttt aaatatggtg accggatttt cacagagtac
      241 ccacaggata ttgtagatta cttcaagaac tcatgtccag cgggctatac gtgggaaagg
      301 agctacctct ttgaggacgg agcggtctgt cgatgcaacg tggacataac agtctctgaa
      361 aaggagaact gcatctatca caaaagtatc ttcagagggg tgaattttcc cgccgacggc
      421 cccgtaatga agaaaatgac cactaattgg gaagctagta ccgagaaaat tgtgcctgtt
      481 ccaaagcaag ggatattaaa gggaaaggtc aaaatgtgcc tgttgctgaa ggatggcggt
      541 cgttatcatt gccagtttga tacggtatat aaagctaaat cagtgccctc caagatgcca
      601 gaatggcatt ttatacagca taagctcctt agggaggacc gctctgatgc taaaaaccag
      661 aaatggcaat taacagaaca tgcaatcgca ggcatggacg agctgtacaa g
//
