LOCUS       sequence-449213-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mKate2, for MuSIC barcode construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, 
            Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR
  TITLE     Genetically Encoded Fluorescence Barcodes Allow for Single-Cell 
            Analysis via Spectral Flow Cytometry.
  JOURNAL   ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 
            10.1021/acssynbio.4c00807. Epub 2025 May 6.
  PUBMED    40326708
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00807"; journalName: "ACS Synth Biol"; date: 
            "2025-05-16- 16"; volume: "14"; issue: "5"; pages: "1533-1548"
COMMENT     Sequence Label: pR-mKate2
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..696
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
ORIGIN
        1 atggtgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
       61 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
      121 accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga catcctggct
      181 accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc
      241 tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata cgaagatggg
      301 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
      361 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
      421 tgggaggcct ccaccgagac actgtacccc gctgacggcg gcctggaagg cagagccgac
      481 atggccctga agctcgtggg cgggggccac ctgatctgca acttaaagac cacatacaga
      541 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaaggctg
      601 gaaagaatca aggaggccga caaagagact tacgtcgagc agcacgaggt ggctgtggcc
      661 agatactgcg acctccctag caaactgggg cacaga
//
