LOCUS       sequence-44922-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Berndsen CE, Wolberger C
  TITLE     A spectrophotometric assay for conjugation of ubiquitin and 
            ubiquitin-like proteins.
  JOURNAL   Anal Biochem. 2011 Nov 1;418(1):102-10. Epub 2011 Jul 2.
  PUBMED    21771579
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2011-11-1"; volume: "418(1)"; pages: "102-10. Epub
            2011 Jul 2"
COMMENT     Sequence Label: Ube1/PET21d
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgcggtgagc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atataccatg ggtcatcacc atcatcatca cggaggtatg
      181 tccagctcgc cgctgtccaa gaaacgtcgc gtgtccgggc ctgatccaaa gccgggttct
      241 aactgctccc ctgcccagtc cgtgttgtcc gaagtgccct cggtgccaac caacggaatg
      301 gccaagaacg gcagtgaagc agacatagac gagggccttt actcccggca gctgtatgtg
      361 ttgggccatg aggcaatgaa gcggctccag acatccagtg tcctggtatc aggcctgcgg
      421 ggcctgggcg tggagatcgc taagaacatc atccttggtg gggtcaaggc tgttacccta
      481 catgaccagg gcactgccca gtgggctgat ctttcctccc agttctacct gcgggaggag
      541 gacatcggta aaaaccgggc cgaggtatca cagccccgcc tcgctgagct caacagctat
      601 gtgcctgtca ctgcctacac tggacccctc gttgaggact tccttagtgg tttccaggtg
      661 gtggtgctca ccaacacccc cctggaggac cagctgcgag tgggtgagtt ctgtcacaac
      721 cgtggcatca agctggtggt ggcagacacg cggggcctgt ttgggcagct cttctgtgac
      781 tttggagagg aaatgatcct cacagattcc aatggggagc agccactcag tgctatggtt
      841 tctatggtta ccaaggacaa ccccggtgtg gttacctgcc tgga
//
