LOCUS       sequence-449225-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode 
            construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, 
            Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR
  TITLE     Genetically Encoded Fluorescence Barcodes Allow for Single-Cell 
            Analysis via Spectral Flow Cytometry.
  JOURNAL   ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 
            10.1021/acssynbio.4c00807. Epub 2025 May 6.
  PUBMED    40326708
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00807"; journalName: "ACS Synth Biol"; date: 
            "2025-05-16- 16"; volume: "14"; issue: "5"; pages: "1533-1548"
COMMENT     Sequence Label: pM-CyOFP1
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..702
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgatcaag gagaacatga gaagcaagct gtacctggaa
       61 ggcagcgtga acggccacca gttcaagtgc acccacgaag gggagggcaa gccctacgag
      121 ggcaagcaga ccaacaggat caaggtggtg gagggaggcc ccctgccgtt cgcattcgac
      181 atcctggcca cccactttat gtacgggagc aaggtgttca tcaagtaccc cgccgacctc
      241 cccgattatt ttaagcagtc cttccctgag ggcttcacat gggagagagt catggtgttc
      301 gaggacgggg gcgtgctgac cgccacccag gacaccagcc tccaggacgg cgagctcatc
      361 tacaacgtca aggtcagagg ggtgaacttc ccagccaacg gccccgtgat gcagaagaaa
      421 acactgggct gggaaccaag caccgaaacc atgtacccag ctgacggagg tctggaagga
      481 agatgcgaca aggccctgaa gctcgtggga ggtggacact tacacgtcaa cttcaagacc
      541 acatacaagt ccaagaaacc cgtgaagatg cccggcgtcc actacgtgga ccgcagactg
      601 gaaagaatca aggaggccga caacgagact tacgtcgagc agtacgagca cgctgtggcc
      661 agatactcca acctgggtgg aggcatggac gagctgtaca ag
//
