LOCUS       sequence-449230-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mRuby3 and extra insert, for MuSIC barcode 
            construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye O, Biswas T, 
            Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR
  TITLE     Genetically Encoded Fluorescence Barcodes Allow for Single-Cell 
            Analysis via Spectral Flow Cytometry.
  JOURNAL   ACS Synth Biol. 2025 May 16;14(5):1533-1548. doi: 
            10.1021/acssynbio.4c00807. Epub 2025 May 6.
  PUBMED    40326708
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00807"; journalName: "ACS Synth Biol"; date: 
            "2025-05-16- 16"; volume: "14"; issue: "5"; pages: "1533-1548"
COMMENT     Sequence Label: pM-mRuby3
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
ORIGIN
        1 atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt ggtcatggaa
       61 ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcag accgtacgag
      121 ggagtgcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac
      181 attcttgcca cgtcgttcat gtatggcagc cgtaccttta tcaagtaccc ggccgacatc
      241 cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt tacgagatac
      301 gaagatggtg gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg cgagctcgtc
      361 tacaacgtca aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaaa
      421 accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg tctgagagga
      481 tacactgaca tcgcactgaa agttgatggt ggtggccatc tgcactgcaa cttcgtgaca
      541 acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtgtcca tgccgttgat
      601 caccgcctgg aaaggatcga ggagagtgac aatgaaacct acgtagtgca aagagaagtg
      661 gcagttgcca aatacagcaa ccttggtggt ggcatggacg agctgtacaa g
//
