LOCUS       sequence-44927-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Almeida MI, Nicoloso MS, Zeng L, Ivan C, Spizzo R, Gafa R, Xiao L, 
            Zhang X, Vannini I, Fanini F, Fabbri M, Lanza G, Reis RM, 
            Zweidler-McKay PA, Calin GA
  TITLE     Strand-Specific miR-28-5p and miR-28-3p Have Distinct Effects in 
            Colorectal Cancer Cells.
  JOURNAL   Gastroenterology. 2012 Jan 10.
  PUBMED    22240480
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Gastroenterology. 2012 Jan 10."
COMMENT     Sequence Label: pBABE-miR-28
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(516..536)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        521..850
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      701..836
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcggacggc cttcaggact ttctagtagt actcaggagt ttgacaaaga ggagctagaa
       61 gaggtttgtg aagtcagatg agtaatataa ttaggtttgt attttatgaa gggcaacatc
      121 taaatatggc ttgtctattc agcaagcact tattaagtgc cttttgcatg gtagacaaca
      181 tgcttgatgc tgaagataca agaaaaaatt taaaatggtc cttgccctca aggagctcac
      241 agtctattga gttacctttc tgactttccc actagattgt gagctcctgg agggcaggca
      301 ctttcgttca tctgaataag agcttaaatt tcagtgttaa tcctagatta caatcccgcc
      361 tctattattt taactttgtt cacatctgtt aactgctctg aaagtcagag ctaatactgc
      421 ctacctcatc agggaaagat taaacgagaa aagaatgaga tgtgattgac acagcaggag
      481 ctctgtgaat tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg
      541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      601 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
      661 caattagtca ccaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      721 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
      781 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      841 cttttgcaaa aagcttacca tgaccgagta caagcccggg c
//
